Galanin modulates vagally induced contractions in the mouse oesophagus

A. Boudaka, J. Wörl, T. Shiina, Y. Shimizu, T. Takewaki, W. L. Neuhuber

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Nitrergic myenteric neurons co-innervating motor endplates were previously shown to inhibit vagally induced contractions of striated muscle in the rodent oesophagus. Immunohistochemical demonstration of putative co-transmitters, e.g. galanin, in enteric neurons prompted us to study a possible role of galanin in modulating vagally mediated contractions in an in vitro vagus nerve-oesophagus preparation of the mouse. Galanin (1-16) (1-100 nmol L-1), in the presence of the peptidase inhibitor, phenanthroline monohydrate, inhibited vagally induced contractions in a concentration-dependent manner (control: 100%; galanin 1 nmol L-1: 95.6 ± 1.6%; galanin 10 nmol L -1: 57.3 ± 6.5%; galanin 100 nmol L-1: 31.2 ± 8.1%, n = 5). The non-selective galanin receptor antagonist, galantide (100 nmol L-1), blocked the inhibitory effect of galanin (10 nmol L-1) while the selective non-galanin receptor 1 and galanin receptor 3 antagonists, M871 (1 μmol L-1) and SNAP37889 (100 nmol L -1), respectively, and the nitric oxide synthase inhibitor, NG-nitro-l-arginine methyl ester (l-NAME) (200 μmol L-1), failed to affect this galanin-induced response. Simultaneous application of galantide (100 nmol L-1) and l-NAME (200 μmol L-1) significantly reduced the inhibitory effect of capsaicin (30 μmol L-1) on vagally induced contractions when compared with its effect in the presence of l-NAME alone or in combination with the selective galanin receptor 2 or 3 antagonists. An inhibitory effect of piperine on vagally induced contractions was reduced neither by galantide nor by l-NAME. Immunohistochemistry revealed galanin immunoreactive myenteric neurons and nerve fibres intermingling with cholinergic vagal terminals at motor endplates. These data suggest that galanin from co-innervating enteric neurons co-operates with nitric oxide in modulating vagally induced contractions in the mouse oesophagus.

Original languageEnglish
Pages (from-to)180-188
Number of pages9
JournalNeurogastroenterology and Motility
Volume21
Issue number2
DOIs
Publication statusPublished - Feb 2009

Fingerprint

Galanin
Esophagus
Receptor, Galanin, Type 3
Motor Endplate
piperine
Neurons
Receptor, Galanin, Type 2
Nitrergic Neurons
Galanin Receptors
Vagus Nerve
Phenanthrolines
Striated Muscle
Capsaicin
Protease Inhibitors
Nerve Fibers
Nitric Oxide Synthase
Cholinergic Agents
Rodentia
Nitric Oxide
Immunohistochemistry

Keywords

  • Capsaicin
  • Co-innervation
  • Enteric neurons
  • Galanin
  • Galantide
  • Motor endplate

ASJC Scopus subject areas

  • Endocrine and Autonomic Systems
  • Gastroenterology
  • Physiology

Cite this

Galanin modulates vagally induced contractions in the mouse oesophagus. / Boudaka, A.; Wörl, J.; Shiina, T.; Shimizu, Y.; Takewaki, T.; Neuhuber, W. L.

In: Neurogastroenterology and Motility, Vol. 21, No. 2, 02.2009, p. 180-188.

Research output: Contribution to journalArticle

Boudaka, A. ; Wörl, J. ; Shiina, T. ; Shimizu, Y. ; Takewaki, T. ; Neuhuber, W. L. / Galanin modulates vagally induced contractions in the mouse oesophagus. In: Neurogastroenterology and Motility. 2009 ; Vol. 21, No. 2. pp. 180-188.
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