GABA, 5-HT and amino acids in the rotifers Brachionus plicatilis and Brachionus rotundiformis

Wenresti G. Gallardo, Atsushi Hagiwara, Kenji Hara, Kiyoshi Soyano, Terry W. Snell

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

γ-Aminobutyric acid (GABA) and 5-hydroxytryptamine (5-HT) have been shown to increase the reproduction of the Brachionus plicatilis (NH3L strain). In the present study, the endogenous presence of GABA and 5-HT in the rotifers B. plicatilis (NH3L and Kamiura strains) and Brachionus rotundiformis (Langkawi strain) were confirmed by dot blot immunoassay and high-performance liquid chromatography (HPLC). HPLC showed that GABA and 5-HT concentrations in the three rotifer strains range from 71 to 188 pmol/mg and from 12 to 64 pmol/mg, respectively. A total of 33 amino acids were also detected in B. plicatilis and B. rotundiformis, with glutamic acid, serine, glycine, taurine, threonine, alanine, arginine, proline, valine and isoleucine in high concentrations relative to other amino acids. (C) 2000 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)301-307
Number of pages7
JournalComparative Biochemistry and Physiology - A Molecular and Integrative Physiology
Volume127
Issue number3
DOIs
Publication statusPublished - 2000

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gamma-Aminobutyric Acid
Serotonin
Amino Acids
High performance liquid chromatography
High Pressure Liquid Chromatography
Aminobutyrates
Isoleucine
Taurine
Valine
Threonine
Immunoassay
Proline
Alanine
Glycine
Serine
Reproduction
Arginine
Glutamic Acid

Keywords

  • 5-HT
  • Amino acids
  • Brachionus
  • Dot blot immunoassay
  • GABA
  • HPLC
  • Neurotransmitters
  • Rotifera
  • Serotonin

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Physiology

Cite this

GABA, 5-HT and amino acids in the rotifers Brachionus plicatilis and Brachionus rotundiformis. / Gallardo, Wenresti G.; Hagiwara, Atsushi; Hara, Kenji; Soyano, Kiyoshi; Snell, Terry W.

In: Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology, Vol. 127, No. 3, 2000, p. 301-307.

Research output: Contribution to journalArticle

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