GABA, 5-HT and amino acids in the rotifers Brachionus plicatilis and Brachionus rotundiformis

Wenresti G. Gallardo; Atsushi Hagiwara; Kenji Hara; Kiyoshi Soyano; Terry W. Snell

doi:10.1016/S1095-6433(00)00266-X

GABA, 5-HT and amino acids in the rotifers Brachionus plicatilis and Brachionus rotundiformis

Wenresti G. Gallardo, Atsushi Hagiwara, Kenji Hara, Kiyoshi Soyano, Terry W. Snell

Marine Science and Fisheries

Research output: Contribution to journal › Article

17 Citations (Scopus)

Abstract

γ-Aminobutyric acid (GABA) and 5-hydroxytryptamine (5-HT) have been shown to increase the reproduction of the Brachionus plicatilis (NH3L strain). In the present study, the endogenous presence of GABA and 5-HT in the rotifers B. plicatilis (NH3L and Kamiura strains) and Brachionus rotundiformis (Langkawi strain) were confirmed by dot blot immunoassay and high-performance liquid chromatography (HPLC). HPLC showed that GABA and 5-HT concentrations in the three rotifer strains range from 71 to 188 pmol/mg and from 12 to 64 pmol/mg, respectively. A total of 33 amino acids were also detected in B. plicatilis and B. rotundiformis, with glutamic acid, serine, glycine, taurine, threonine, alanine, arginine, proline, valine and isoleucine in high concentrations relative to other amino acids. (C) 2000 Elsevier Science Inc.

Original language	English
Pages (from-to)	301-307
Number of pages	7
Journal	Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology
Volume	127
Issue number	3
DOIs	https://doi.org/10.1016/S1095-6433(00)00266-X
Publication status	Published - 2000

Keywords

5-HT
Amino acids
Brachionus
Dot blot immunoassay
GABA
HPLC
Neurotransmitters
Rotifera
Serotonin

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Physiology

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10.1016/S1095-6433(00)00266-X

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Gallardo, W. G., Hagiwara, A., Hara, K., Soyano, K., & Snell, T. W. (2000). GABA, 5-HT and amino acids in the rotifers Brachionus plicatilis and Brachionus rotundiformis. Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology, 127(3), 301-307. https://doi.org/10.1016/S1095-6433(00)00266-X

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abstract = "γ-Aminobutyric acid (GABA) and 5-hydroxytryptamine (5-HT) have been shown to increase the reproduction of the Brachionus plicatilis (NH3L strain). In the present study, the endogenous presence of GABA and 5-HT in the rotifers B. plicatilis (NH3L and Kamiura strains) and Brachionus rotundiformis (Langkawi strain) were confirmed by dot blot immunoassay and high-performance liquid chromatography (HPLC). HPLC showed that GABA and 5-HT concentrations in the three rotifer strains range from 71 to 188 pmol/mg and from 12 to 64 pmol/mg, respectively. A total of 33 amino acids were also detected in B. plicatilis and B. rotundiformis, with glutamic acid, serine, glycine, taurine, threonine, alanine, arginine, proline, valine and isoleucine in high concentrations relative to other amino acids. (C) 2000 Elsevier Science Inc.",

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AU - Soyano, Kiyoshi

AU - Snell, Terry W.

PY - 2000

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N2 - γ-Aminobutyric acid (GABA) and 5-hydroxytryptamine (5-HT) have been shown to increase the reproduction of the Brachionus plicatilis (NH3L strain). In the present study, the endogenous presence of GABA and 5-HT in the rotifers B. plicatilis (NH3L and Kamiura strains) and Brachionus rotundiformis (Langkawi strain) were confirmed by dot blot immunoassay and high-performance liquid chromatography (HPLC). HPLC showed that GABA and 5-HT concentrations in the three rotifer strains range from 71 to 188 pmol/mg and from 12 to 64 pmol/mg, respectively. A total of 33 amino acids were also detected in B. plicatilis and B. rotundiformis, with glutamic acid, serine, glycine, taurine, threonine, alanine, arginine, proline, valine and isoleucine in high concentrations relative to other amino acids. (C) 2000 Elsevier Science Inc.

AB - γ-Aminobutyric acid (GABA) and 5-hydroxytryptamine (5-HT) have been shown to increase the reproduction of the Brachionus plicatilis (NH3L strain). In the present study, the endogenous presence of GABA and 5-HT in the rotifers B. plicatilis (NH3L and Kamiura strains) and Brachionus rotundiformis (Langkawi strain) were confirmed by dot blot immunoassay and high-performance liquid chromatography (HPLC). HPLC showed that GABA and 5-HT concentrations in the three rotifer strains range from 71 to 188 pmol/mg and from 12 to 64 pmol/mg, respectively. A total of 33 amino acids were also detected in B. plicatilis and B. rotundiformis, with glutamic acid, serine, glycine, taurine, threonine, alanine, arginine, proline, valine and isoleucine in high concentrations relative to other amino acids. (C) 2000 Elsevier Science Inc.

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KW - Neurotransmitters

KW - Rotifera

KW - Serotonin

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