TY - JOUR
T1 - GABA, 5-HT and amino acids in the rotifers Brachionus plicatilis and Brachionus rotundiformis
AU - Gallardo, Wenresti G.
AU - Hagiwara, Atsushi
AU - Hara, Kenji
AU - Soyano, Kiyoshi
AU - Snell, Terry W.
N1 - Funding Information:
Amino acid analysis by HPLC was done at Waters’ laboratory facilities in Osaka, Japan. This study was supported by Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan (No. 10660187) and Integrated Research Program for Effects of Endocrine Disrupters on Agriculture, Forestry, and Fisheries and their Action Mechanisms on Domestic Animals and Fishes (ED-99-II-3-1).
PY - 2000
Y1 - 2000
N2 - γ-Aminobutyric acid (GABA) and 5-hydroxytryptamine (5-HT) have been shown to increase the reproduction of the Brachionus plicatilis (NH3L strain). In the present study, the endogenous presence of GABA and 5-HT in the rotifers B. plicatilis (NH3L and Kamiura strains) and Brachionus rotundiformis (Langkawi strain) were confirmed by dot blot immunoassay and high-performance liquid chromatography (HPLC). HPLC showed that GABA and 5-HT concentrations in the three rotifer strains range from 71 to 188 pmol/mg and from 12 to 64 pmol/mg, respectively. A total of 33 amino acids were also detected in B. plicatilis and B. rotundiformis, with glutamic acid, serine, glycine, taurine, threonine, alanine, arginine, proline, valine and isoleucine in high concentrations relative to other amino acids. (C) 2000 Elsevier Science Inc.
AB - γ-Aminobutyric acid (GABA) and 5-hydroxytryptamine (5-HT) have been shown to increase the reproduction of the Brachionus plicatilis (NH3L strain). In the present study, the endogenous presence of GABA and 5-HT in the rotifers B. plicatilis (NH3L and Kamiura strains) and Brachionus rotundiformis (Langkawi strain) were confirmed by dot blot immunoassay and high-performance liquid chromatography (HPLC). HPLC showed that GABA and 5-HT concentrations in the three rotifer strains range from 71 to 188 pmol/mg and from 12 to 64 pmol/mg, respectively. A total of 33 amino acids were also detected in B. plicatilis and B. rotundiformis, with glutamic acid, serine, glycine, taurine, threonine, alanine, arginine, proline, valine and isoleucine in high concentrations relative to other amino acids. (C) 2000 Elsevier Science Inc.
KW - 5-HT
KW - Amino acids
KW - Brachionus
KW - Dot blot immunoassay
KW - GABA
KW - HPLC
KW - Neurotransmitters
KW - Rotifera
KW - Serotonin
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U2 - 10.1016/S1095-6433(00)00266-X
DO - 10.1016/S1095-6433(00)00266-X
M3 - Article
C2 - 11118940
AN - SCOPUS:0033638094
VL - 127
SP - 301
EP - 307
JO - Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology
JF - Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology
SN - 1095-6433
IS - 3
ER -