Abstract
Using a dual color ELISPOT assay able to detect HIV-specific IFN-γ, IL-2 and dual IFN-γ/IL-2 secreting lymphocytes we screened for HIV peptide-specific responses directed against the entire HIV proteome in two groups of untreated HIV-infected individuals, slow progressors (SP) and progressors. We found that the three functional lymphocyte subsets contributed differentially to individual HIV peptide-specific responses within a study subject. Among the identified stimulatory peptides, a higher proportion induced dual IFN-γ/IL-2 secretion in SP than progressors. While the magnitude of single IFN-γ secreting lymphocytes is similar between groups, the magnitude of peptide-specific dual IFN-γ/IL-2 secreting lymphocytes is significantly more intense in SP. Neither single nor total IFN-γ secreting cell magnitude and breadth measurements correlated with CD4 cell count or viral load whereas both parameters of dual IFN-γ/IL-2 secreting responses correlated positively with CD4 counts and negatively with viremia.
Original language | English |
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Pages (from-to) | 57-68 |
Number of pages | 12 |
Journal | Clinical Immunology |
Volume | 124 |
Issue number | 1 |
DOIs | |
Publication status | Published - Jul 2007 |
Keywords
- AIDS
- Cellular immunology
- Disease progression
- Dual color ELISPOT
- Functional subsets
- HIV
- Long term non progressors
- T cells
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology