Abstract
Using a dual color ELISPOT assay able to detect HIV-specific IFN-γ, IL-2 and dual IFN-γ/IL-2 secreting lymphocytes we screened for HIV peptide-specific responses directed against the entire HIV proteome in two groups of untreated HIV-infected individuals, slow progressors (SP) and progressors. We found that the three functional lymphocyte subsets contributed differentially to individual HIV peptide-specific responses within a study subject. Among the identified stimulatory peptides, a higher proportion induced dual IFN-γ/IL-2 secretion in SP than progressors. While the magnitude of single IFN-γ secreting lymphocytes is similar between groups, the magnitude of peptide-specific dual IFN-γ/IL-2 secreting lymphocytes is significantly more intense in SP. Neither single nor total IFN-γ secreting cell magnitude and breadth measurements correlated with CD4 cell count or viral load whereas both parameters of dual IFN-γ/IL-2 secreting responses correlated positively with CD4 counts and negatively with viremia.
| Original language | English |
|---|---|
| Pages (from-to) | 57-68 |
| Number of pages | 12 |
| Journal | Clinical Immunology |
| Volume | 124 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jul 2007 |
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Keywords
- AIDS
- Cellular immunology
- Disease progression
- Dual color ELISPOT
- Functional subsets
- HIV
- Long term non progressors
- T cells
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
Cite this
Functional T cell subsets contribute differentially to HIV peptide-specific responses within infected individuals : Correlation of these functional T cell subsets with markers of disease progression. / Peretz, Yoav; Ndongala, Michel L.; Boulet, Salix; Boulassel, Mohamed R.; Rouleau, Danielle; Côté, Pierre; Longpré, Danièle; Routy, Jean Pierre; Falutz, Julian; Tremblay, Cécile; Tsoukas, Christos M.; Sékaly, Rafick P.; Bernard, Nicole F.
In: Clinical Immunology, Vol. 124, No. 1, 07.2007, p. 57-68.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Functional T cell subsets contribute differentially to HIV peptide-specific responses within infected individuals
T2 - Correlation of these functional T cell subsets with markers of disease progression
AU - Peretz, Yoav
AU - Ndongala, Michel L.
AU - Boulet, Salix
AU - Boulassel, Mohamed R.
AU - Rouleau, Danielle
AU - Côté, Pierre
AU - Longpré, Danièle
AU - Routy, Jean Pierre
AU - Falutz, Julian
AU - Tremblay, Cécile
AU - Tsoukas, Christos M.
AU - Sékaly, Rafick P.
AU - Bernard, Nicole F.
PY - 2007/7
Y1 - 2007/7
N2 - Using a dual color ELISPOT assay able to detect HIV-specific IFN-γ, IL-2 and dual IFN-γ/IL-2 secreting lymphocytes we screened for HIV peptide-specific responses directed against the entire HIV proteome in two groups of untreated HIV-infected individuals, slow progressors (SP) and progressors. We found that the three functional lymphocyte subsets contributed differentially to individual HIV peptide-specific responses within a study subject. Among the identified stimulatory peptides, a higher proportion induced dual IFN-γ/IL-2 secretion in SP than progressors. While the magnitude of single IFN-γ secreting lymphocytes is similar between groups, the magnitude of peptide-specific dual IFN-γ/IL-2 secreting lymphocytes is significantly more intense in SP. Neither single nor total IFN-γ secreting cell magnitude and breadth measurements correlated with CD4 cell count or viral load whereas both parameters of dual IFN-γ/IL-2 secreting responses correlated positively with CD4 counts and negatively with viremia.
AB - Using a dual color ELISPOT assay able to detect HIV-specific IFN-γ, IL-2 and dual IFN-γ/IL-2 secreting lymphocytes we screened for HIV peptide-specific responses directed against the entire HIV proteome in two groups of untreated HIV-infected individuals, slow progressors (SP) and progressors. We found that the three functional lymphocyte subsets contributed differentially to individual HIV peptide-specific responses within a study subject. Among the identified stimulatory peptides, a higher proportion induced dual IFN-γ/IL-2 secretion in SP than progressors. While the magnitude of single IFN-γ secreting lymphocytes is similar between groups, the magnitude of peptide-specific dual IFN-γ/IL-2 secreting lymphocytes is significantly more intense in SP. Neither single nor total IFN-γ secreting cell magnitude and breadth measurements correlated with CD4 cell count or viral load whereas both parameters of dual IFN-γ/IL-2 secreting responses correlated positively with CD4 counts and negatively with viremia.
KW - AIDS
KW - Cellular immunology
KW - Disease progression
KW - Dual color ELISPOT
KW - Functional subsets
KW - HIV
KW - Long term non progressors
KW - T cells
UR - http://www.scopus.com/inward/record.url?scp=34250652387&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34250652387&partnerID=8YFLogxK
U2 - 10.1016/j.clim.2007.04.004
DO - 10.1016/j.clim.2007.04.004
M3 - Article
VL - 124
SP - 57
EP - 68
JO - Clinical Immunology
JF - Clinical Immunology
SN - 1521-6616
IS - 1
ER -