@article{562c6f089f444594b4a186c5d4719885,
title = "Functional T cell subsets contribute differentially to HIV peptide-specific responses within infected individuals: Correlation of these functional T cell subsets with markers of disease progression",
abstract = "Using a dual color ELISPOT assay able to detect HIV-specific IFN-γ, IL-2 and dual IFN-γ/IL-2 secreting lymphocytes we screened for HIV peptide-specific responses directed against the entire HIV proteome in two groups of untreated HIV-infected individuals, slow progressors (SP) and progressors. We found that the three functional lymphocyte subsets contributed differentially to individual HIV peptide-specific responses within a study subject. Among the identified stimulatory peptides, a higher proportion induced dual IFN-γ/IL-2 secretion in SP than progressors. While the magnitude of single IFN-γ secreting lymphocytes is similar between groups, the magnitude of peptide-specific dual IFN-γ/IL-2 secreting lymphocytes is significantly more intense in SP. Neither single nor total IFN-γ secreting cell magnitude and breadth measurements correlated with CD4 cell count or viral load whereas both parameters of dual IFN-γ/IL-2 secreting responses correlated positively with CD4 counts and negatively with viremia.",
keywords = "AIDS, Cellular immunology, Disease progression, Dual color ELISPOT, Functional subsets, HIV, Long term non progressors, T cells",
author = "Yoav Peretz and Ndongala, {Michel L.} and Salix Boulet and Boulassel, {Mohamed R.} and Danielle Rouleau and Pierre C{\^o}t{\'e} and Dani{\`e}le Longpr{\'e} and Routy, {Jean Pierre} and Julian Falutz and C{\'e}cile Tremblay and Tsoukas, {Christos M.} and S{\'e}kaly, {Rafick P.} and Bernard, {Nicole F.}",
note = "Funding Information: The authors wish to thank the study participants at the Immuno-Deficiency Treatment Center and the Montreal LTNP and Primary Infection (PI) cohorts. We wish to acknowledge Drs. Howard Turner, Jason Szabo, R{\'e}jean Thomas, Jean-Guy Baril, Vim-Kim Nguyen, Sylvie V{\'e}zina and Jean-Pierre Maziade for study participant recruitment, Ms. Nancy Simic and Marie-Pierre Boisvert for expert technical assistance, Ms. Louise Gilbert for clinical data management, Ms Chantal Grignon for nursing support for the leukapheresis infrastructure and Mr. Mario Legault and Ms. Linda Racicot, coordinators of the PI and LTNP cohorts. We also wish to thank Mr. Tameem Ansari at Cellular Technology Ltd. for help with troubleshooting the reading of dual color ELISPOTs. This work was funded by the R{\'e}seau du Sida et Maladies Infectieuses du Fonds de la Recherche en Sant{\'e} du Qu{\'e}bec (FRSQ) and by a grant from the Canadian Institutes for Health Research #MOP79515.",
year = "2007",
month = jul,
doi = "10.1016/j.clim.2007.04.004",
language = "English",
volume = "124",
pages = "57--68",
journal = "Clinical Immunology",
issn = "1521-6616",
publisher = "Academic Press Inc.",
number = "1",
}