Functional, histological and biomechanical characterization of wheat water-mutant leaves

Agata Rascio, Nicoletta Rascio, Michele Rinaldi, Massimiliano Valentini

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A wheat (Triticum turgidum subsp. durum) mutant, generated with sodium azide from wild-type (WT) cv. 'Trinakria', differs in its water affinity of dry leaves, and was designated as a water-mutant. Compared with the WT, water-mutant leaves have lower rates of water uptake, while stomatal and cuticular transpiration do not differ. The nuclear magnetic resonance proton signals used for image reconstruction of leaf cross sections showed differences between these genotypes for the T1 proton spin-density and the T2 proton spin-spin relaxation time. Structural and histochemical analyses at midrib level showed that the water-mutant has thinner leaves, with more and smaller cells per unit area of mesophyll and sclerenchyma, and has altered staining patterns of lignin and pectin-like substances. Stress-strain curves to examine the rheological properties of the leaves showed a biphasic trend, which reveals that the tensile strength at break load and the elastic modulus of the second phase of the water-mutant are significantly higher than for the WT. These data support the proposal of interrelationships among local biophysical properties of the leaf, the microscopic water structure, the rheological properties and the water flux rate across the leaf. This water-mutant can be used for analysis of the genetic basis of these differences, and for identification of gene(s) that govern these traits.

Original languageEnglish
Pages (from-to)210-222
Number of pages13
JournalPhysiologia Plantarum
Volume154
Issue number2
DOIs
Publication statusPublished - Jun 1 2015

Fingerprint

Triticum
mutants
wheat
Water
leaves
water
protons
Protons
rheological properties
sclerenchyma
sodium azide
Triticum turgidum subsp. durum
Sodium Azide
Computer-Assisted Image Processing
tensile strength
Tensile Strength
Lignin
Elastic Modulus
modulus of elasticity
mesophyll

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Functional, histological and biomechanical characterization of wheat water-mutant leaves. / Rascio, Agata; Rascio, Nicoletta; Rinaldi, Michele; Valentini, Massimiliano.

In: Physiologia Plantarum, Vol. 154, No. 2, 01.06.2015, p. 210-222.

Research output: Contribution to journalArticle

Rascio, Agata ; Rascio, Nicoletta ; Rinaldi, Michele ; Valentini, Massimiliano. / Functional, histological and biomechanical characterization of wheat water-mutant leaves. In: Physiologia Plantarum. 2015 ; Vol. 154, No. 2. pp. 210-222.
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