Functional characterization of a soybean growth stimulator Bradyrhizobium sp. strain SR-6 showing acylhomoserine lactone production

Amanat Ali, Ayesha, Sohail Hameed, Asma Imran, Mazhar Iqbal, Javed Iqbal, Ivan J. Oresnik

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A soybean nodule endophytic bacterium Bradyrhizobium sp. strain SR-6 was characterized for production of acyl homoserine lactones (AHLs) as quorum sensing molecules. Mass spectrometry analysis of AHLs revealed the presence of C6-HSL, 3OH-C6-HSL, C8-HSL, C10-HSL, 3oxoC10-HSL, 3oxo-C12-HSL and 3OH-C12-HSL which are significantly different from those reported earlier in soybean symbionts. Purified AHL extracts significantly improved wheat and soybean seedling growth and root hair development along with increased soybean nodulation under axenic conditions. A positive correlation was observed among in vivo nitrogenase and catalase enzyme activities of the strain SR-6. Transmission electron microscopic analysis showed the cytochemical localization of catalase activity within the bacteroids, specifically attached to the peribacteroidal membrane. Root and nodule colonization proved rhizosphere competence of SR-6. The inoculation of SR-6 resulted in increased shoot length (13%), plant dry matter (50%), grain weight (16%), seed yield (20%) and N-uptake (14%) as compared to non-inoculated soybean plants. The symbiotic bacterium SR-6 has potential to improve soybean growth and yield in sub-humid climate of Azad Jammu and Kashmir region of Pakistan. The production and mass spectrometric profiling of AHLs as well as in vivo cytochemical localization of catalase enzyme activity in soybean Bradyrhizobium sp. have never been reported earlier elsewhere before our these investigations.

Original languageEnglish
Article numberfiw115
JournalFEMS Microbiology Ecology
Volume92
Issue number9
DOIs
Publication statusPublished - Sep 1 2016

Fingerprint

Bradyrhizobium
Lactones
Soybeans
soybean
Acyl-Butyrolactones
Growth
Catalase
enzyme activity
Bacteria
Nitrogenase
Quorum Sensing
Rhizosphere
bacterium
nodulation
Pakistan
Enzymes
symbiont
Climate
Seedlings
hair

Keywords

  • Acyl homoserine lactones
  • Bradyrhizobium
  • Catalase enzyme
  • Nitrogenase activity
  • Soybean

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Functional characterization of a soybean growth stimulator Bradyrhizobium sp. strain SR-6 showing acylhomoserine lactone production. / Ali, Amanat; Ayesha; Hameed, Sohail; Imran, Asma; Iqbal, Mazhar; Iqbal, Javed; Oresnik, Ivan J.

In: FEMS Microbiology Ecology, Vol. 92, No. 9, fiw115, 01.09.2016.

Research output: Contribution to journalArticle

Ali, Amanat ; Ayesha ; Hameed, Sohail ; Imran, Asma ; Iqbal, Mazhar ; Iqbal, Javed ; Oresnik, Ivan J. / Functional characterization of a soybean growth stimulator Bradyrhizobium sp. strain SR-6 showing acylhomoserine lactone production. In: FEMS Microbiology Ecology. 2016 ; Vol. 92, No. 9.
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