Freezing tolerance in Norway spruce, the potential role of pathogenesis-related proteins

Lars Sandved Dalen, Øystein Johnsen, Anders Lönneborg, Mahmoud W. Yaish

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cold-tolerant plants may endure subzero temperatures partially by inhibiting the development of ice crystals in the intercellular spaces and the xylem through the accumulation of antifreeze proteins (AFP) and the extra production of carbohydrates. Certain proteins associated with pathogen resistance in plants have the ability to bind and alter the growth of ice crystals. In this study, the accumulation of pathogenesis-related (PR) proteins and the development of freezing tolerance in seedlings of two latitudinal distinct Norway spruce (Picea abies L. Karst.) ecotypes were investigated. Despite freezing tolerance difference, timing of growth cessation and bud set variations, our results showed that there is no significant difference in the concentration of soluble carbohydrates between the two ecotypes. Immunoblots showed the presence of several β-1,3-glucanase and thaumatin PR proteins in the apoplastic fluid and the enzymatic assay showed an extra accumulation of several isoforms of PR chitinases in cold-treated Norway spruce needles. In addition to PR proteins, a presence of de novo protein in cold-treated needles was noticed. In contrary to mature plants, total proteins isolated from freezing-tolerant Norway spruce seedling did not show antifreeze activity. Our results suggest that the activity of the PR proteins and the accumulation of soluble carbohydrates that increased during cold acclimation may have an indirect impact on the freezing tolerance in Norway spruce, however, deciphering the direct mechanism behind freezing tolerance in Norway spruce seedlings growing under controlled environmental conditions require further investigation.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalActa Physiologiae Plantarum
Volume37
Issue number1
DOIs
Publication statusPublished - Nov 19 2014

Fingerprint

pathogenesis-related proteins
Norway
cold tolerance
Freezing
Picea abies
Seedlings
Ecotype
Proteins
Ice
ecotypes
Needles
crystals
seedlings
ice
Antifreeze Proteins
Carbohydrates
Abies
Picea
carbohydrates
Xylem

Keywords

  • Antifreeze proteins
  • Carbohydrates
  • Chitinases
  • Norway spruce

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Agronomy and Crop Science

Cite this

Freezing tolerance in Norway spruce, the potential role of pathogenesis-related proteins. / Dalen, Lars Sandved; Johnsen, Øystein; Lönneborg, Anders; Yaish, Mahmoud W.

In: Acta Physiologiae Plantarum, Vol. 37, No. 1, 19.11.2014, p. 1-9.

Research output: Contribution to journalArticle

Dalen, Lars Sandved ; Johnsen, Øystein ; Lönneborg, Anders ; Yaish, Mahmoud W. / Freezing tolerance in Norway spruce, the potential role of pathogenesis-related proteins. In: Acta Physiologiae Plantarum. 2014 ; Vol. 37, No. 1. pp. 1-9.
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