Fractured carbonate reservoirs sweep efficiency improvement using microbial biomass

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7 Citations (Scopus)

Abstract

Selective plugging by microbial biomass is one of the proposed mechanisms for improving reservoir sweep efficiency in fractured reservoirs. In this study, the potential of Bacillus licheniformis strains isolated from oil contaminated soil from the Sultanate of Oman was tested for their ability to grow in induced fractures in carbonate rocks and to divert subsequent injection water to the unswept matrix zones.Three B. licheniformis strains were tested with name codes; B29, B17 and W16. Their growth behavior using different nitrogen sources - yeast extract, peptone and urea - was investigated. Glucose, sucrose and date molasses were tested as carbon sources. Carbon/nitrogen ratios were optimized where it was found that sucrose was the carbon source that maximized bacterial growth at 2% concentration and yeast extract was the selected nitrogen source with concentration of 0.1%. The combination of B. licheniformis strain W16 in a minimal medium containing sucrose was the optimum condition for maximum cell growth within 10-12. h of incubation. Standard Indiana limestone core plugs were used for coreflooding experiments where a fracture was simulated by slicing the cores vertically into two sections using a thin blade. The bacterial cells were injected into the cores and the ability of the microbes to grow and plug the fracture was examined. Scanning electron microscopy was used to prove the growth of the microbial cells in the fracture after the experiment.Coreflooding experiments showed promising results where enhancement of oil recovery was observed after bacterial injection. A total of 27-30% of the residual oil was produced after 11. h of incubation. This shows the high potential of using microbial biomass for selective plugging in fractured reservoirs.

Original languageEnglish
Pages (from-to)178-184
Number of pages7
JournalJournal of Petroleum Science and Engineering
Volume112
DOIs
Publication statusPublished - Dec 2013

Fingerprint

Carbonates
Sugar (sucrose)
Biomass
sucrose
carbonate
biomass
Nitrogen
Yeast
Carbon
yeast
oil
nitrogen
carbon
incubation
Molasses
Water injection
experiment
Experiments
Cell growth
Bacilli

Keywords

  • Bacillus licheniformis
  • Core flooding
  • Enhanced oil recovery
  • Selective plugging

ASJC Scopus subject areas

  • Geotechnical Engineering and Engineering Geology
  • Fuel Technology

Cite this

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title = "Fractured carbonate reservoirs sweep efficiency improvement using microbial biomass",
abstract = "Selective plugging by microbial biomass is one of the proposed mechanisms for improving reservoir sweep efficiency in fractured reservoirs. In this study, the potential of Bacillus licheniformis strains isolated from oil contaminated soil from the Sultanate of Oman was tested for their ability to grow in induced fractures in carbonate rocks and to divert subsequent injection water to the unswept matrix zones.Three B. licheniformis strains were tested with name codes; B29, B17 and W16. Their growth behavior using different nitrogen sources - yeast extract, peptone and urea - was investigated. Glucose, sucrose and date molasses were tested as carbon sources. Carbon/nitrogen ratios were optimized where it was found that sucrose was the carbon source that maximized bacterial growth at 2{\%} concentration and yeast extract was the selected nitrogen source with concentration of 0.1{\%}. The combination of B. licheniformis strain W16 in a minimal medium containing sucrose was the optimum condition for maximum cell growth within 10-12. h of incubation. Standard Indiana limestone core plugs were used for coreflooding experiments where a fracture was simulated by slicing the cores vertically into two sections using a thin blade. The bacterial cells were injected into the cores and the ability of the microbes to grow and plug the fracture was examined. Scanning electron microscopy was used to prove the growth of the microbial cells in the fracture after the experiment.Coreflooding experiments showed promising results where enhancement of oil recovery was observed after bacterial injection. A total of 27-30{\%} of the residual oil was produced after 11. h of incubation. This shows the high potential of using microbial biomass for selective plugging in fractured reservoirs.",
keywords = "Bacillus licheniformis, Core flooding, Enhanced oil recovery, Selective plugging",
author = "Rayah Al-Hattali and Hanaa Al-Sulaimani and Yahya Al-Wahaibi and Saif Al-Bahry and Abdulkader Elshafie and Ali Al-Bemani and Joshi, {Sanket J.}",
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AU - Al-Hattali, Rayah

AU - Al-Sulaimani, Hanaa

AU - Al-Wahaibi, Yahya

AU - Al-Bahry, Saif

AU - Elshafie, Abdulkader

AU - Al-Bemani, Ali

AU - Joshi, Sanket J.

PY - 2013/12

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N2 - Selective plugging by microbial biomass is one of the proposed mechanisms for improving reservoir sweep efficiency in fractured reservoirs. In this study, the potential of Bacillus licheniformis strains isolated from oil contaminated soil from the Sultanate of Oman was tested for their ability to grow in induced fractures in carbonate rocks and to divert subsequent injection water to the unswept matrix zones.Three B. licheniformis strains were tested with name codes; B29, B17 and W16. Their growth behavior using different nitrogen sources - yeast extract, peptone and urea - was investigated. Glucose, sucrose and date molasses were tested as carbon sources. Carbon/nitrogen ratios were optimized where it was found that sucrose was the carbon source that maximized bacterial growth at 2% concentration and yeast extract was the selected nitrogen source with concentration of 0.1%. The combination of B. licheniformis strain W16 in a minimal medium containing sucrose was the optimum condition for maximum cell growth within 10-12. h of incubation. Standard Indiana limestone core plugs were used for coreflooding experiments where a fracture was simulated by slicing the cores vertically into two sections using a thin blade. The bacterial cells were injected into the cores and the ability of the microbes to grow and plug the fracture was examined. Scanning electron microscopy was used to prove the growth of the microbial cells in the fracture after the experiment.Coreflooding experiments showed promising results where enhancement of oil recovery was observed after bacterial injection. A total of 27-30% of the residual oil was produced after 11. h of incubation. This shows the high potential of using microbial biomass for selective plugging in fractured reservoirs.

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