Foliar application of Burkholderia sp. strain TNAU-1 leads to activation of defense responses in chilli (Capsicum annuum L.)

Srinivasan Madhavan, Vaikuntavasan Paranidharan, Rethinasamy Velazhahan*

*Corresponding author for this work

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Induction of phenolics, peroxidase (PO), polyphenol oxidase (PPO), chitinase and thaumatin-like proteins (TLP) in leaves of chilli (Capsicum annuum L.) in response to foliar application with a biocontrol agent Burkholderia sp. strain TNAU-1 was studied. Chilli plants, when sprayed with Burkholderia sp. strain TNAU-1 showed increase in phenolic content one day after application and the maximum accumulation was observed seven days after treatment. Three new peroxidase isozymes (PO-1, PO-2 and PO-3) were induced in chilli leaves upon treatment with Burkholderia sp. strain TNAU-1. The activity of all the peroxidases was at the maximum level three days after treatment and subsequently decreased. Protein extracts obtained from Burkholderia sp. strain TNAU-1 treated plants exhibited a major polyphenol oxidase (PPO-1) one day after treatment. The activity of PPO-1 subsequently decreased, but continuously present throughout the experimental period of 15 days. Western blot analysis of protein extracts from Burkholderia sp. strain TNAU-1 treated chilli leaves revealed that two TLPs with sizes of 23 and 25 kDa and a chitinase with an apparent molecular weight of 28 kDa were induced three days after treatment.

Original languageEnglish
Pages (from-to)261-266
Number of pages6
JournalBrazilian Journal of Plant Physiology
Volume23
Issue number4
Publication statusPublished - 2011

Keywords

  • Capsicum annuum
  • Chitinase
  • Induced resistance
  • Peroxidase
  • Phenolics
  • Polyphenol oxidase
  • Thaumatin-like protein

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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