Abstract
Induction of phenolics, peroxidase (PO), polyphenol oxidase (PPO), chitinase and thaumatin-like proteins (TLP) in leaves of chilli (Capsicum annuum L.) in response to foliar application with a biocontrol agent Burkholderia sp. strain TNAU-1 was studied. Chilli plants, when sprayed with Burkholderia sp. strain TNAU-1 showed increase in phenolic content one day after application and the maximum accumulation was observed seven days after treatment. Three new peroxidase isozymes (PO-1, PO-2 and PO-3) were induced in chilli leaves upon treatment with Burkholderia sp. strain TNAU-1. The activity of all the peroxidases was at the maximum level three days after treatment and subsequently decreased. Protein extracts obtained from Burkholderia sp. strain TNAU-1 treated plants exhibited a major polyphenol oxidase (PPO-1) one day after treatment. The activity of PPO-1 subsequently decreased, but continuously present throughout the experimental period of 15 days. Western blot analysis of protein extracts from Burkholderia sp. strain TNAU-1 treated chilli leaves revealed that two TLPs with sizes of 23 and 25 kDa and a chitinase with an apparent molecular weight of 28 kDa were induced three days after treatment.
Original language | English |
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Pages (from-to) | 261-266 |
Number of pages | 6 |
Journal | Brazilian Journal of Plant Physiology |
Volume | 23 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2011 |
Externally published | Yes |
Keywords
- Capsicum annuum
- Chitinase
- Induced resistance
- Peroxidase
- Phenolics
- Polyphenol oxidase
- Thaumatin-like protein
ASJC Scopus subject areas
- Agronomy and Crop Science
- Plant Science