Fluorescence characterization of water-driven self-assembled lipids and their temperature-induced phase transitions

N. Idayu Zahid, Osama K. Abou-Zied, Rauzah Hashim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Water-driven self-assembly of lipids displays a variety of liquid crystalline phases that are crucial for membrane functions. In this work, we characterized the temperature-induced phase transitions in aqueous self-assembly systems using steady-state and time-resolved fluorescence measurements. The polar head group region was investigated using tryptophan (Trp) and two of its ester derivatives, and the hydrophobic tail region was probed using pyrene. The spectral changes in tryptophan and pyrene were used as a benchmark to estimate the polarity of the head group region and the tail region, respectively. A basic medium was detected and estimated for the polar region in the inverse cubic phase of a Guerbet glycolipid and was attributed to the structural effect of the narrow nanochannels. All the studied lipid compositions show completely reversible temperature-induced phase transitions, reflecting the thermodynamic equilibrium structures of their mesophases. The results reveal a large degree of heterogeneity and flexibility of the lipid self-assembly which may be crucial for carrying out different biological functions.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
PublisherSPIE
Volume9339
ISBN (Print)9781628414295
DOIs
Publication statusPublished - 2015
EventReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VII - San Francisco, United States
Duration: Feb 8 2015Feb 10 2015

Other

OtherReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VII
CountryUnited States
CitySan Francisco
Period2/8/152/10/15

Keywords

  • fluorescence
  • Glycolipid
  • heterogeneity
  • lifetime
  • lyotropic
  • nanochannels
  • pyrene
  • tryptophan

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Zahid, N. I., Abou-Zied, O. K., & Hashim, R. (2015). Fluorescence characterization of water-driven self-assembled lipids and their temperature-induced phase transitions. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 9339). [93390L] SPIE. https://doi.org/10.1117/12.2083677