Fluorescence characterization of water-driven self-assembled lipids and their temperature-induced phase transitions

N. Idayu Zahid, Osama K. Abou-Zied, Rauzah Hashim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Water-driven self-assembly of lipids displays a variety of liquid crystalline phases that are crucial for membrane functions. In this work, we characterized the temperature-induced phase transitions in aqueous self-assembly systems using steady-state and time-resolved fluorescence measurements. The polar head group region was investigated using tryptophan (Trp) and two of its ester derivatives, and the hydrophobic tail region was probed using pyrene. The spectral changes in tryptophan and pyrene were used as a benchmark to estimate the polarity of the head group region and the tail region, respectively. A basic medium was detected and estimated for the polar region in the inverse cubic phase of a Guerbet glycolipid and was attributed to the structural effect of the narrow nanochannels. All the studied lipid compositions show completely reversible temperature-induced phase transitions, reflecting the thermodynamic equilibrium structures of their mesophases. The results reveal a large degree of heterogeneity and flexibility of the lipid self-assembly which may be crucial for carrying out different biological functions.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
PublisherSPIE
Volume9339
ISBN (Print)9781628414295
DOIs
Publication statusPublished - 2015
EventReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VII - San Francisco, United States
Duration: Feb 8 2015Feb 10 2015

Other

OtherReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VII
CountryUnited States
CitySan Francisco
Period2/8/152/10/15

Fingerprint

Phase Transition
Self assembly
Lipids
lipids
self assembly
Fluorescence
Phase transitions
tryptophan
Pyrene
pyrenes
Tryptophan
fluorescence
Temperature
Water
water
Cold Climate
Benchmarking
Glycolipids
thermodynamic equilibrium
Thermodynamics

Keywords

  • fluorescence
  • Glycolipid
  • heterogeneity
  • lifetime
  • lyotropic
  • nanochannels
  • pyrene
  • tryptophan

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Zahid, N. I., Abou-Zied, O. K., & Hashim, R. (2015). Fluorescence characterization of water-driven self-assembled lipids and their temperature-induced phase transitions. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 9339). [93390L] SPIE. https://doi.org/10.1117/12.2083677

Fluorescence characterization of water-driven self-assembled lipids and their temperature-induced phase transitions. / Zahid, N. Idayu; Abou-Zied, Osama K.; Hashim, Rauzah.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 9339 SPIE, 2015. 93390L.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Zahid, NI, Abou-Zied, OK & Hashim, R 2015, Fluorescence characterization of water-driven self-assembled lipids and their temperature-induced phase transitions. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 9339, 93390L, SPIE, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VII, San Francisco, United States, 2/8/15. https://doi.org/10.1117/12.2083677
Zahid NI, Abou-Zied OK, Hashim R. Fluorescence characterization of water-driven self-assembled lipids and their temperature-induced phase transitions. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 9339. SPIE. 2015. 93390L https://doi.org/10.1117/12.2083677
Zahid, N. Idayu ; Abou-Zied, Osama K. ; Hashim, Rauzah. / Fluorescence characterization of water-driven self-assembled lipids and their temperature-induced phase transitions. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 9339 SPIE, 2015.
@inproceedings{7f70dff9b2d443529a0b015ae4aaa7d1,
title = "Fluorescence characterization of water-driven self-assembled lipids and their temperature-induced phase transitions",
abstract = "Water-driven self-assembly of lipids displays a variety of liquid crystalline phases that are crucial for membrane functions. In this work, we characterized the temperature-induced phase transitions in aqueous self-assembly systems using steady-state and time-resolved fluorescence measurements. The polar head group region was investigated using tryptophan (Trp) and two of its ester derivatives, and the hydrophobic tail region was probed using pyrene. The spectral changes in tryptophan and pyrene were used as a benchmark to estimate the polarity of the head group region and the tail region, respectively. A basic medium was detected and estimated for the polar region in the inverse cubic phase of a Guerbet glycolipid and was attributed to the structural effect of the narrow nanochannels. All the studied lipid compositions show completely reversible temperature-induced phase transitions, reflecting the thermodynamic equilibrium structures of their mesophases. The results reveal a large degree of heterogeneity and flexibility of the lipid self-assembly which may be crucial for carrying out different biological functions.",
keywords = "fluorescence, Glycolipid, heterogeneity, lifetime, lyotropic, nanochannels, pyrene, tryptophan",
author = "Zahid, {N. Idayu} and Abou-Zied, {Osama K.} and Rauzah Hashim",
year = "2015",
doi = "10.1117/12.2083677",
language = "English",
isbn = "9781628414295",
volume = "9339",
booktitle = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",

}

TY - GEN

T1 - Fluorescence characterization of water-driven self-assembled lipids and their temperature-induced phase transitions

AU - Zahid, N. Idayu

AU - Abou-Zied, Osama K.

AU - Hashim, Rauzah

PY - 2015

Y1 - 2015

N2 - Water-driven self-assembly of lipids displays a variety of liquid crystalline phases that are crucial for membrane functions. In this work, we characterized the temperature-induced phase transitions in aqueous self-assembly systems using steady-state and time-resolved fluorescence measurements. The polar head group region was investigated using tryptophan (Trp) and two of its ester derivatives, and the hydrophobic tail region was probed using pyrene. The spectral changes in tryptophan and pyrene were used as a benchmark to estimate the polarity of the head group region and the tail region, respectively. A basic medium was detected and estimated for the polar region in the inverse cubic phase of a Guerbet glycolipid and was attributed to the structural effect of the narrow nanochannels. All the studied lipid compositions show completely reversible temperature-induced phase transitions, reflecting the thermodynamic equilibrium structures of their mesophases. The results reveal a large degree of heterogeneity and flexibility of the lipid self-assembly which may be crucial for carrying out different biological functions.

AB - Water-driven self-assembly of lipids displays a variety of liquid crystalline phases that are crucial for membrane functions. In this work, we characterized the temperature-induced phase transitions in aqueous self-assembly systems using steady-state and time-resolved fluorescence measurements. The polar head group region was investigated using tryptophan (Trp) and two of its ester derivatives, and the hydrophobic tail region was probed using pyrene. The spectral changes in tryptophan and pyrene were used as a benchmark to estimate the polarity of the head group region and the tail region, respectively. A basic medium was detected and estimated for the polar region in the inverse cubic phase of a Guerbet glycolipid and was attributed to the structural effect of the narrow nanochannels. All the studied lipid compositions show completely reversible temperature-induced phase transitions, reflecting the thermodynamic equilibrium structures of their mesophases. The results reveal a large degree of heterogeneity and flexibility of the lipid self-assembly which may be crucial for carrying out different biological functions.

KW - fluorescence

KW - Glycolipid

KW - heterogeneity

KW - lifetime

KW - lyotropic

KW - nanochannels

KW - pyrene

KW - tryptophan

UR - http://www.scopus.com/inward/record.url?scp=84931843510&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84931843510&partnerID=8YFLogxK

U2 - 10.1117/12.2083677

DO - 10.1117/12.2083677

M3 - Conference contribution

SN - 9781628414295

VL - 9339

BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

PB - SPIE

ER -