First report of sweet potato leaf spot caused by Neopestalotiopsis ellipsospora in Guizhou Province, China

S. S N Maharachchikumbura, S. P. Wu, K. D. Hyde, A. M. Al-Sadi, Z. Y. Liu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

During May to September 2015, sweet potato [Ipomoea batatas (L.) Lam.] plants with leaf spot symptoms were observed in Guizhou Province, China, at 25°12’06.8”N 107°57’25.3”E with about 15% incidence. The disease first appeared as small, round, yellow specks on leaves. Successively, the affected areas became necrotic and gradually enlarged to 3-9 mm in diameter. The fungal species present on the sample was isolated onto PDA using a single spore culture technique as described in Chomnunti et al. (2014). Colonies on PDA attained 30-40 mm in diameter after 7 days at 25°C, producing a dense aerial mycelium on the surface. Conidia were ellipsoid, 4-septate, 20.0-26.0 × 5.0-7.5 μm. Apical and basal cells were hyaline, whereas the three median cells were versicolored. Each apical cell had 3 tubular apical appendages, 7-24 μm long. The conidial dimensions of these isolates correspond to Pestalotiopsis ellipsospora Maharachchikumbura & K.D. Hyde (Maharachchikumbura et al., 2012). Sequence data for the rDNA internal transcribed spacer (ITS), Beta tubulin (TUB) and partial translation elongation factor 1-alpha (TEF) of three N. ellipsospora isolates were deposited in GenBank under the accession Nos. KU500017 to KU500019 (ITS), KU500010 to KU500012 (TUB) and KU500013 to KU500015 (TEF). A BLAST search showed 100% similarity with N. ellipsospora isolate (CBS 115113) sequences deposited in GenBank. The disease was reproduced on sweet potato healthy leaves inoculated using either 3-day-old mycelial discs or inoculum (1× 106 conidia/ml) prepared by scraping conidia from 10-day-old cultures on PDA plates. To our knowledge this is the first report of Neopestalotiopsis causing leaf spot on sweet potatoes in the world.

Original languageEnglish
Pages (from-to)686
Number of pages1
JournalJournal of Plant Pathology
Volume98
Issue number3
Publication statusPublished - 2016

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leaf spot
sweet potatoes
conidia
tubulin
translation (genetics)
internal transcribed spacers
China
Pestalotiopsis
Ipomoea batatas
cells
appendages
signs and symptoms (plants)
mycelium
leaves
inoculum
spores
incidence
Neopestalotiopsis
sampling
methodology

ASJC Scopus subject areas

  • Plant Science

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First report of sweet potato leaf spot caused by Neopestalotiopsis ellipsospora in Guizhou Province, China. / Maharachchikumbura, S. S N; Wu, S. P.; Hyde, K. D.; Al-Sadi, A. M.; Liu, Z. Y.

In: Journal of Plant Pathology, Vol. 98, No. 3, 2016, p. 686.

Research output: Contribution to journalArticle

Maharachchikumbura, S. S N ; Wu, S. P. ; Hyde, K. D. ; Al-Sadi, A. M. ; Liu, Z. Y. / First report of sweet potato leaf spot caused by Neopestalotiopsis ellipsospora in Guizhou Province, China. In: Journal of Plant Pathology. 2016 ; Vol. 98, No. 3. pp. 686.
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