Fast HPLC analysis of adenine in human plasma using a new generation C 28 column and different extraction methods

Mohammed Al Za'Abi, Badreldin H. Ali, Afzal Hussain, Imran Ali

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A fast, simple, inexpensive, selective, rugged and reproducible HPLC method based on the use of a new generation Sunniest RP-Aqua C28 column is described for the analysis of adenine in plasma. Three methods for the extraction of adenine from human plasma i.e. by acetone, acetonitrile and solid phase cartridges are reported. The separation was performed via isocratic elution of adenine using water-acetonitrile (90:10, v/v) as mobile phase at a flow rate of 1.0 mL min-1 and with detection at 260 nm. The values of retention factor in standard and plasma samples were 2.53 and 2.60, respectively. The method was validated and found to be linear at a concentration range of 5-1000 μg mL-1. The limits of detection and quantification were lower than 0.3 and 0.91 μg mL-1, respectively. The intra- and inter-day precisions (% RSD) were less than 9%. The accuracy values were between 99.8 and 107.4%. The extraction recoveries ranged from 95-80%.

Original languageEnglish
Pages (from-to)1487-1493
Number of pages7
JournalAnalytical Methods
Volume5
Issue number6
DOIs
Publication statusPublished - Mar 21 2013

Fingerprint

Plasma (human)
Adenine
Acetonitrile
Plasmas
Acetone
Flow rate
Recovery
Water
acetonitrile

ASJC Scopus subject areas

  • Analytical Chemistry
  • Engineering(all)
  • Chemical Engineering(all)

Cite this

Fast HPLC analysis of adenine in human plasma using a new generation C 28 column and different extraction methods. / Al Za'Abi, Mohammed; Ali, Badreldin H.; Hussain, Afzal; Ali, Imran.

In: Analytical Methods, Vol. 5, No. 6, 21.03.2013, p. 1487-1493.

Research output: Contribution to journalArticle

@article{0afb5a237e2c4aa7958e7a8639947a5b,
title = "Fast HPLC analysis of adenine in human plasma using a new generation C 28 column and different extraction methods",
abstract = "A fast, simple, inexpensive, selective, rugged and reproducible HPLC method based on the use of a new generation Sunniest RP-Aqua C28 column is described for the analysis of adenine in plasma. Three methods for the extraction of adenine from human plasma i.e. by acetone, acetonitrile and solid phase cartridges are reported. The separation was performed via isocratic elution of adenine using water-acetonitrile (90:10, v/v) as mobile phase at a flow rate of 1.0 mL min-1 and with detection at 260 nm. The values of retention factor in standard and plasma samples were 2.53 and 2.60, respectively. The method was validated and found to be linear at a concentration range of 5-1000 μg mL-1. The limits of detection and quantification were lower than 0.3 and 0.91 μg mL-1, respectively. The intra- and inter-day precisions ({\%} RSD) were less than 9{\%}. The accuracy values were between 99.8 and 107.4{\%}. The extraction recoveries ranged from 95-80{\%}.",
author = "{Al Za'Abi}, Mohammed and Ali, {Badreldin H.} and Afzal Hussain and Imran Ali",
year = "2013",
month = "3",
day = "21",
doi = "10.1039/c2ay26363g",
language = "English",
volume = "5",
pages = "1487--1493",
journal = "Analytical Methods",
issn = "1759-9660",
publisher = "Royal Society of Chemistry",
number = "6",

}

TY - JOUR

T1 - Fast HPLC analysis of adenine in human plasma using a new generation C 28 column and different extraction methods

AU - Al Za'Abi, Mohammed

AU - Ali, Badreldin H.

AU - Hussain, Afzal

AU - Ali, Imran

PY - 2013/3/21

Y1 - 2013/3/21

N2 - A fast, simple, inexpensive, selective, rugged and reproducible HPLC method based on the use of a new generation Sunniest RP-Aqua C28 column is described for the analysis of adenine in plasma. Three methods for the extraction of adenine from human plasma i.e. by acetone, acetonitrile and solid phase cartridges are reported. The separation was performed via isocratic elution of adenine using water-acetonitrile (90:10, v/v) as mobile phase at a flow rate of 1.0 mL min-1 and with detection at 260 nm. The values of retention factor in standard and plasma samples were 2.53 and 2.60, respectively. The method was validated and found to be linear at a concentration range of 5-1000 μg mL-1. The limits of detection and quantification were lower than 0.3 and 0.91 μg mL-1, respectively. The intra- and inter-day precisions (% RSD) were less than 9%. The accuracy values were between 99.8 and 107.4%. The extraction recoveries ranged from 95-80%.

AB - A fast, simple, inexpensive, selective, rugged and reproducible HPLC method based on the use of a new generation Sunniest RP-Aqua C28 column is described for the analysis of adenine in plasma. Three methods for the extraction of adenine from human plasma i.e. by acetone, acetonitrile and solid phase cartridges are reported. The separation was performed via isocratic elution of adenine using water-acetonitrile (90:10, v/v) as mobile phase at a flow rate of 1.0 mL min-1 and with detection at 260 nm. The values of retention factor in standard and plasma samples were 2.53 and 2.60, respectively. The method was validated and found to be linear at a concentration range of 5-1000 μg mL-1. The limits of detection and quantification were lower than 0.3 and 0.91 μg mL-1, respectively. The intra- and inter-day precisions (% RSD) were less than 9%. The accuracy values were between 99.8 and 107.4%. The extraction recoveries ranged from 95-80%.

UR - http://www.scopus.com/inward/record.url?scp=84874640574&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84874640574&partnerID=8YFLogxK

U2 - 10.1039/c2ay26363g

DO - 10.1039/c2ay26363g

M3 - Article

AN - SCOPUS:84874640574

VL - 5

SP - 1487

EP - 1493

JO - Analytical Methods

JF - Analytical Methods

SN - 1759-9660

IS - 6

ER -