Expression profiling of pathogenesis-related Protein-1 (PR-1) genes from Solanum tuberosum reveals its critical role in phytophthora infestans infection

Madiha Zaynab, Jiaofeng Peng, Yasir Sharif, Rashid Al-Yahyai, Atka Jamil, Athar Hussain, Khalid Ali Khan, Saqer S. Alotaibi, Shuangfei Li*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Pathogen-related (PR) proteins are an integral part of plants' defense mechanisms against various types of biotic and abiotic stresses. A little is known about the importance of these PR proteins in potato defense mechanisms. In the current study, a total of 22 pathogenesis-related 1 genes were identified in the potato genome. All identified proteins possessed the CAP superfamily domain with some other motifs. The cis-acting elements analysis identified several stress-responsive elements, including MYB, ABRE, and MeJRE. The gene duplication events demonstrated purifying and positive selection pressure. Expression profiling showed high transcripts level in root compared to other tissues; however, some genes have tissue-specific expression. Furthermore, the PR-1-5 gene is transcriptionally induced under Phytophthora infestans stress and hormonal (ABA and IAA) treatments. The Real-Time qPCR analysis also validated the RNA-seq data results of genes with maximum expression in roots compared to leaves and stems. The current study results provided basic data for functional characterization and can also use as a reference study for other important crops.

Original languageEnglish
Article number105290
JournalMicrobial Pathogenesis
Volume161
DOIs
Publication statusPublished - Dec 2021

Keywords

  • Defense
  • Expression
  • Immune system
  • Pathogen
  • RT-qPCR

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

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