Expression of the genes coding for the xylanase Xys1 and the cellulase Cel1 from the straw-decomposing Streptomyces halstedii JM8 cloned into the amino-acid producer Brevibacterium lactofermentum ATCC13869

Sirin A. Adham; Pilar Honrubia; Margarita Díaz; José M. Fernández-Abalos; Ramón I. Santamaría; José A. Gil

doi:10.1007/s00203-001-0365-3

Expression of the genes coding for the xylanase Xys1 and the cellulase Cel1 from the straw-decomposing Streptomyces halstedii JM8 cloned into the amino-acid producer Brevibacterium lactofermentum ATCC13869

Sirin A. Adham, Pilar Honrubia, Margarita Díaz, José M. Fernández-Abalos, Ramón I. Santamaría, José A. Gil^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

The xylanase (xysA) and the cellulase (celA1) genes from Streptomyces halstedii JM8 were cloned into Escherichia coli/Brevibacterium lactofermentum shuttle vectors and successfully expressed in both hosts when placed downstream from the kanamycin resistance promoter (Pkan) from Tn5 but not when under the control of their own promoters. Xylanase was secreted into the culture media of B. lactofermentum by removal of the same leader peptide as is removed in S. halstedii. The main difference between the production of xylanase by Streptomyces and corynebacteria was the low level of processing of the mature extracellular xylanase by B. lactofermentum, probably due to the lack of protease activity in this microorganism.

Original language	English
Pages (from-to)	91-97
Number of pages	7
Journal	Archives of Microbiology
Volume	177
Issue number	1
DOIs	https://doi.org/10.1007/s00203-001-0365-3
Publication status	Published - Dec 2001
Externally published	Yes

Keywords

Cellulase
Corynebacteria
Leader peptide
Proteolytic processing
Secretion
Xylanase

ASJC Scopus subject areas

Microbiology
Biochemistry
Molecular Biology
Genetics

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Adham, S. A., Honrubia, P., Díaz, M., Fernández-Abalos, J. M., Santamaría, R. I., & Gil, J. A. (2001). Expression of the genes coding for the xylanase Xys1 and the cellulase Cel1 from the straw-decomposing Streptomyces halstedii JM8 cloned into the amino-acid producer Brevibacterium lactofermentum ATCC13869. Archives of Microbiology, 177(1), 91-97. https://doi.org/10.1007/s00203-001-0365-3

Expression of the genes coding for the xylanase Xys1 and the cellulase Cel1 from the straw-decomposing Streptomyces halstedii JM8 cloned into the amino-acid producer Brevibacterium lactofermentum ATCC13869. / Adham, Sirin A.; Honrubia, Pilar; Díaz, Margarita et al.
In: Archives of Microbiology, Vol. 177, No. 1, 12.2001, p. 91-97.

Research output: Contribution to journal › Article › peer-review

Adham, SA, Honrubia, P, Díaz, M, Fernández-Abalos, JM, Santamaría, RI & Gil, JA 2001, 'Expression of the genes coding for the xylanase Xys1 and the cellulase Cel1 from the straw-decomposing Streptomyces halstedii JM8 cloned into the amino-acid producer Brevibacterium lactofermentum ATCC13869', Archives of Microbiology, vol. 177, no. 1, pp. 91-97. https://doi.org/10.1007/s00203-001-0365-3

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title = "Expression of the genes coding for the xylanase Xys1 and the cellulase Cel1 from the straw-decomposing Streptomyces halstedii JM8 cloned into the amino-acid producer Brevibacterium lactofermentum ATCC13869",

abstract = "The xylanase (xysA) and the cellulase (celA1) genes from Streptomyces halstedii JM8 were cloned into Escherichia coli/Brevibacterium lactofermentum shuttle vectors and successfully expressed in both hosts when placed downstream from the kanamycin resistance promoter (Pkan) from Tn5 but not when under the control of their own promoters. Xylanase was secreted into the culture media of B. lactofermentum by removal of the same leader peptide as is removed in S. halstedii. The main difference between the production of xylanase by Streptomyces and corynebacteria was the low level of processing of the mature extracellular xylanase by B. lactofermentum, probably due to the lack of protease activity in this microorganism.",

keywords = "Cellulase, Corynebacteria, Leader peptide, Proteolytic processing, Secretion, Xylanase",

author = "Adham, {Sirin A.} and Pilar Honrubia and Margarita D{\'i}az and Fern{\'a}ndez-Abalos, {Jos{\'e} M.} and Santamar{\'i}a, {Ram{\'o}n I.} and Gil, {Jos{\'e} A.}",

note = "Funding Information: Acknowledgements This work was funded by a grant from the European Community-Ministerio de Ciencia y Tecnolog{\'i}a (1-FD1997-1134C-03). S. A. Adham was recipient of a fellowship from the Agencia Espa{\~n}ola de Cooperaci{\'o}n Internacional (AECI). We thank Dr. Ana B. Campelo (University of Le{\'o}n, Spain) for her help in some parts of this work. The experiments described in this work comply with current Spanish legislation.",

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AU - Adham, Sirin A.

AU - Honrubia, Pilar

AU - Díaz, Margarita

AU - Fernández-Abalos, José M.

AU - Santamaría, Ramón I.

AU - Gil, José A.

N1 - Funding Information: Acknowledgements This work was funded by a grant from the European Community-Ministerio de Ciencia y Tecnología (1-FD1997-1134C-03). S. A. Adham was recipient of a fellowship from the Agencia Española de Cooperación Internacional (AECI). We thank Dr. Ana B. Campelo (University of León, Spain) for her help in some parts of this work. The experiments described in this work comply with current Spanish legislation.

PY - 2001/12

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N2 - The xylanase (xysA) and the cellulase (celA1) genes from Streptomyces halstedii JM8 were cloned into Escherichia coli/Brevibacterium lactofermentum shuttle vectors and successfully expressed in both hosts when placed downstream from the kanamycin resistance promoter (Pkan) from Tn5 but not when under the control of their own promoters. Xylanase was secreted into the culture media of B. lactofermentum by removal of the same leader peptide as is removed in S. halstedii. The main difference between the production of xylanase by Streptomyces and corynebacteria was the low level of processing of the mature extracellular xylanase by B. lactofermentum, probably due to the lack of protease activity in this microorganism.

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Expression of the genes coding for the xylanase Xys1 and the cellulase Cel1 from the straw-decomposing Streptomyces halstedii JM8 cloned into the amino-acid producer Brevibacterium lactofermentum ATCC13869

Abstract

Keywords

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