Expression of a Rice Thaumatin-Like Protein (PR-5) Gene in a Baculovirus Expression System

R. Velazhahan, K. C. Zen, S. Muthukrishnan

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We previously isolated a full-length thaumatin-like protein (TLP) cDNA clone (D34) from a rice cDNA library prepared from RNA isolated from R. solani-infected rice plants. Furthermore, it has been demonstrated that transgenic rice plants that are constitutively expressing the TLP (D34) show enhanced resistance to R. solani. In order to purify and characterize this TLP. we expressed it (D34) in Spodoptera frugiperda (sf21) insect cells using a baculovirus expression system. Western blot analysis indicated that a 23-kDa protein cross-reacting with a bean TLP antibody was produced abundantly in the insect cells. However, this protein was not secreted into the culture medium. The N-terminal sequence of the protein (19 residues) showed high sequence similarity to thaumalin and TLPs of oat, barley, maize, grapes and tomato. The cleavage site for the TLP was determined as immediately upstream of the sequence ATFAITN.

Original languageEnglish
Pages (from-to)311-316
Number of pages6
JournalActa Phytopathologica et Entomologica Hungarica
Volume36
Issue number3-4
DOIs
Publication statusPublished - 2001

Fingerprint

Baculoviridae
rice
genes
proteins
insects
Spodoptera frugiperda
cDNA libraries
oats
grapes
beans
Western blotting
amino acid sequences
culture media
barley
genetically modified organisms
tomatoes
RNA
cells
clones
antibodies

Keywords

  • N-terminal sequence
  • Oryza sativa
  • Pathogenesis-related protein

ASJC Scopus subject areas

  • Plant Science
  • Insect Science

Cite this

Expression of a Rice Thaumatin-Like Protein (PR-5) Gene in a Baculovirus Expression System. / Velazhahan, R.; Zen, K. C.; Muthukrishnan, S.

In: Acta Phytopathologica et Entomologica Hungarica, Vol. 36, No. 3-4, 2001, p. 311-316.

Research output: Contribution to journalArticle

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