Exploitation of potential target tissues to develop polyploids in citrus

Muhammad Usman, Bilquees Fatima, Kazim Ali Gillani, Muhammad Sarwar Khan, Muhammad Mumtaz Khan

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

One of the rich sources of germplasm development to improve citrus crop is the ploidy management through in vivo and in vitro techniques. In vitro and in vivo applications of colchicine change the chromosome number by interfering with chromosomal segregation at anaphase, restricting cell wall formation and yielding polyploid cells. Further, endosperm culture under aseptic conditions also allows regeneration of plants with variable genetic make up. We report here the development of polyploids through in vitro culturing of endosperm and application of colchicine. Somatic embryos were developed from 'Kinnow' mandarin (Citrus reticulata L.) and 'Succari' sweet orange (Citrus sinensis L. Osbeck) on basal medium modified with higher concentrations of BA and NAA (10mgL -1 and 2mgL -1, respectively) from calli induced on Murashige and Tucker (MT) medium supplemented with BA, 2,4-D, KIN, ME and CH. Recovery of polyploids from endosperm culture was significantly low (32%) compared to colchicine induced ploids (53.67%). Bud sprouting percentage and shoot length was found inversely proportional while leaf size was directly proportional to colchicine application. The stomatal studies of the regenerated plants revealed them as polyploids. These strategies are promising techniques for production of citrus polyploids, however; further genetic characterization of the ploids is suggested for future breeding and biotechnology applications. Abbreviations: BA 6-benzaylaminopurine, CH Casein hydrolysate, KIN Kinetin, ME Malt Extract; NAA 1-naphthaleneacetic acid, 2,4-D 2,4-Dichlorophenoxyacetic acid.

Original languageEnglish
Pages (from-to)1755-1766
Number of pages12
JournalPakistan Journal of Botany
Volume40
Issue number4 SPEC. ISS.
Publication statusPublished - Aug 2008

Fingerprint

polyploidy
colchicine
Citrus
naphthaleneacetic acid
2,4-D
endosperm
Citrus reticulata
casein hydrolysates
malt extract
aseptic conditions
anaphase
mandarins
Citrus sinensis
sprouting
kinetin
somatic embryos
ploidy
biotechnology
chromosome number
callus

ASJC Scopus subject areas

  • Plant Science

Cite this

Usman, M., Fatima, B., Gillani, K. A., Khan, M. S., & Khan, M. M. (2008). Exploitation of potential target tissues to develop polyploids in citrus. Pakistan Journal of Botany, 40(4 SPEC. ISS.), 1755-1766.

Exploitation of potential target tissues to develop polyploids in citrus. / Usman, Muhammad; Fatima, Bilquees; Gillani, Kazim Ali; Khan, Muhammad Sarwar; Khan, Muhammad Mumtaz.

In: Pakistan Journal of Botany, Vol. 40, No. 4 SPEC. ISS., 08.2008, p. 1755-1766.

Research output: Contribution to journalArticle

Usman, M, Fatima, B, Gillani, KA, Khan, MS & Khan, MM 2008, 'Exploitation of potential target tissues to develop polyploids in citrus', Pakistan Journal of Botany, vol. 40, no. 4 SPEC. ISS., pp. 1755-1766.
Usman M, Fatima B, Gillani KA, Khan MS, Khan MM. Exploitation of potential target tissues to develop polyploids in citrus. Pakistan Journal of Botany. 2008 Aug;40(4 SPEC. ISS.):1755-1766.
Usman, Muhammad ; Fatima, Bilquees ; Gillani, Kazim Ali ; Khan, Muhammad Sarwar ; Khan, Muhammad Mumtaz. / Exploitation of potential target tissues to develop polyploids in citrus. In: Pakistan Journal of Botany. 2008 ; Vol. 40, No. 4 SPEC. ISS. pp. 1755-1766.
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