Existence of thaumatin-like proteins (TLPs) in seeds of cereals

R. Radhajeyalakshmi, K. Yamunarani, K. Seetharaman, R. Velazhahan

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Seed extracts of pearl millet, sorghum, Japanese barnyard millet, foxtail millet, samai and proso millet were evaluated in vitro for their ability to inhibit the growth of Rhizoctonia solani, Macrophomina phaseolina and Fusarium oxysporum. Among them, seed extracts of pearl millet and sorghum were highly effective in inhibiting the growth of all three examined phytopathogenic fungi. The seed extracts were tested for the presence of thaumatin-like proteins (TLPs) by Western blot analysis using bean TLP antiserum. Results of Western blot analysis indicated the presence of a 23-kDa TLP in seeds of pearl millet, sorghum and Japanese barnyard millet. The 23-kDa TLP was more abundant in the seeds of pearl millet. The distribution of TLP in various parts of pearl millet was analyzed by Western blotting. The results indicated that the 23 kDa TLP was predominantly expressed in seeds and inflorescence of pearl millet.

Original languageEnglish
Pages (from-to)251-257
Number of pages7
JournalActa Phytopathologica et Entomologica Hungarica
Volume38
Issue number3-4
DOIs
Publication statusPublished - 2003

Fingerprint

Pennisetum glaucum
seed extracts
seeds
Echinochloa frumentacea
Sorghum (Poaceae)
Western blotting
proteins
Panicum miliaceum subsp. miliaceum
Setaria italica
Macrophomina phaseolina
plant pathogenic fungi
Thanatephorus cucumeris
Fusarium oxysporum
antiserum
beans
inflorescences

Keywords

  • Antifungal protein
  • Pathogenesis-related protein
  • Pearl millet
  • Pennisetum glaucum

ASJC Scopus subject areas

  • Plant Science
  • Insect Science

Cite this

Existence of thaumatin-like proteins (TLPs) in seeds of cereals. / Radhajeyalakshmi, R.; Yamunarani, K.; Seetharaman, K.; Velazhahan, R.

In: Acta Phytopathologica et Entomologica Hungarica, Vol. 38, No. 3-4, 2003, p. 251-257.

Research output: Contribution to journalArticle

Radhajeyalakshmi, R. ; Yamunarani, K. ; Seetharaman, K. ; Velazhahan, R. / Existence of thaumatin-like proteins (TLPs) in seeds of cereals. In: Acta Phytopathologica et Entomologica Hungarica. 2003 ; Vol. 38, No. 3-4. pp. 251-257.
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