Evolutionarily assembled cis-regulatory module at a human ciliopathy locus

Jeong Ho Lee; Jennifer L. Silhavy; Ji Eun Lee; Lihadh Al-Gazali; Sophie Thomas; Erica E. Davis; Stephanie L. Bielas; Kiley J. Hill; Miriam Iannicelli; Francesco Brancati; Stacey B. Gabriel; Carsten Russ; Clare V. Logan; Saghira Malik Sharif; Christopher P. Bennett; Masumi Abe; Friedhelm Hildebrandt; Bill H. Diplas; Tania Attié-Bitach; Nicholas Katsanis; Anna Rajab; Roshan Koul; Laszlo Sztriha; Elizabeth R. Waters; Susan Ferro-Novick; C. Geoffrey Woods; Colin A. Johnson; Enza Maria Valente; Maha S. Zaki; Joseph G. Gleeson

doi:10.1126/science.1213506

Evolutionarily assembled cis-regulatory module at a human ciliopathy locus

Jeong Ho Lee, Jennifer L. Silhavy, Ji Eun Lee, Lihadh Al-Gazali, Sophie Thomas, Erica E. Davis, Stephanie L. Bielas, Kiley J. Hill, Miriam Iannicelli, Francesco Brancati, Stacey B. Gabriel, Carsten Russ, Clare V. Logan, Saghira Malik Sharif, Christopher P. Bennett, Masumi Abe, Friedhelm Hildebrandt, Bill H. Diplas, Tania Attié-Bitach, Nicholas KatsanisAnna Rajab, Roshan Koul, Laszlo Sztriha, Elizabeth R. Waters, Susan Ferro-Novick, C. Geoffrey Woods, Colin A. Johnson, Enza Maria Valente, Maha S. Zaki, Joseph G. Gleeson^*

^*Corresponding author for this work

Child Health

Research output: Contribution to journal › Article › peer-review

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Abstract

Neighboring genes are often coordinately expressed within cis-regulatory modules, but evidence that nonparalogous genes share functions in mammals is lacking. Here, we report that mutation of either TMEM138 or TMEM216 causes a phenotypically indistinguishable human ciliopathy, Joubert syndrome. Despite a lack of sequence homology, the genes are aligned in a head-to-tail configuration and joined by chromosomal rearrangement at the amphibian-to-reptile evolutionary transition. Expression of the two genes is mediated by a conserved regulatory element in the noncoding intergenic region. Coordinated expression is important for their interdependent cellular role in vesicular transport to primary cilia. Hence, during vertebrate evolution of genes involved in ciliogenesis, nonparalogous genes were arranged to a functional gene cluster with shared regulatory elements.

Original language	English
Pages (from-to)	966-969
Number of pages	4
Journal	Science
Volume	335
Issue number	6071
DOIs	https://doi.org/10.1126/science.1213506
Publication status	Published - Feb 24 2012

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Lee, J. H., Silhavy, J. L., Lee, J. E., Al-Gazali, L., Thomas, S., Davis, E. E., Bielas, S. L., Hill, K. J., Iannicelli, M., Brancati, F., Gabriel, S. B., Russ, C., Logan, C. V., Sharif, S. M., Bennett, C. P., Abe, M., Hildebrandt, F., Diplas, B. H., Attié-Bitach, T., ... Gleeson, J. G. (2012). Evolutionarily assembled cis-regulatory module at a human ciliopathy locus. Science, 335(6071), 966-969. https://doi.org/10.1126/science.1213506

Lee, JH, Silhavy, JL, Lee, JE, Al-Gazali, L, Thomas, S, Davis, EE, Bielas, SL, Hill, KJ, Iannicelli, M, Brancati, F, Gabriel, SB, Russ, C, Logan, CV, Sharif, SM, Bennett, CP, Abe, M, Hildebrandt, F, Diplas, BH, Attié-Bitach, T, Katsanis, N, Rajab, A, Koul, R, Sztriha, L, Waters, ER, Ferro-Novick, S, Woods, CG, Johnson, CA, Valente, EM, Zaki, MS & Gleeson, JG 2012, 'Evolutionarily assembled cis-regulatory module at a human ciliopathy locus', Science, vol. 335, no. 6071, pp. 966-969. https://doi.org/10.1126/science.1213506

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title = "Evolutionarily assembled cis-regulatory module at a human ciliopathy locus",

abstract = "Neighboring genes are often coordinately expressed within cis-regulatory modules, but evidence that nonparalogous genes share functions in mammals is lacking. Here, we report that mutation of either TMEM138 or TMEM216 causes a phenotypically indistinguishable human ciliopathy, Joubert syndrome. Despite a lack of sequence homology, the genes are aligned in a head-to-tail configuration and joined by chromosomal rearrangement at the amphibian-to-reptile evolutionary transition. Expression of the two genes is mediated by a conserved regulatory element in the noncoding intergenic region. Coordinated expression is important for their interdependent cellular role in vesicular transport to primary cilia. Hence, during vertebrate evolution of genes involved in ciliogenesis, nonparalogous genes were arranged to a functional gene cluster with shared regulatory elements.",

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AU - Hildebrandt, Friedhelm

AU - Diplas, Bill H.

AU - Attié-Bitach, Tania

AU - Katsanis, Nicholas

AU - Rajab, Anna

AU - Koul, Roshan

AU - Sztriha, Laszlo

AU - Waters, Elizabeth R.

AU - Ferro-Novick, Susan

AU - Woods, C. Geoffrey

AU - Johnson, Colin A.

AU - Valente, Enza Maria

AU - Zaki, Maha S.

AU - Gleeson, Joseph G.

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N2 - Neighboring genes are often coordinately expressed within cis-regulatory modules, but evidence that nonparalogous genes share functions in mammals is lacking. Here, we report that mutation of either TMEM138 or TMEM216 causes a phenotypically indistinguishable human ciliopathy, Joubert syndrome. Despite a lack of sequence homology, the genes are aligned in a head-to-tail configuration and joined by chromosomal rearrangement at the amphibian-to-reptile evolutionary transition. Expression of the two genes is mediated by a conserved regulatory element in the noncoding intergenic region. Coordinated expression is important for their interdependent cellular role in vesicular transport to primary cilia. Hence, during vertebrate evolution of genes involved in ciliogenesis, nonparalogous genes were arranged to a functional gene cluster with shared regulatory elements.

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Evolutionarily assembled cis-regulatory module at a human ciliopathy locus

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