Evolution of rabbit haemorrhagic disease virus (RHDV) in the European rabbit (Oryctolagus cuniculus) from the Iberian Peninsula

A. Muller, J. Freitas, E. Silva, G. Le Gall-Reculé, F. Zwingelstein, J. Abrantes, P. J. Esteves, P. C. Alves, W. van der Loo, J. Kolodziejek, N. Nowotny, G. Thompson

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

To date information on rabbit haemorrhagic disease virus (RHDV) in Spain and Portugal has been scarce, although the disease is endemic and continues to have a considerable impact on species conservation and hunting industry. We analysed RHDVs obtained between 1994 and 2007 at different geographic locations in Portugal (40 samples), Spain (3 samples) and France (4 samples) from wild European rabbits (Oryctolagus cuniculus) that succumbed to the disease. Phylogenetic analyses based on partial VP60 gene sequences allowed a grouping of these RHDVs into three groups, termed "Iberian" Groups IB1, IB2 and IB3. Interestingly, these three Iberian groups clustered separately, though not far from earlier RHDVs of Genogroup 1 (containing e.g., strain "AST89"), but clearly distinct from globally described RHDV strains of Genogroups 2-6. This result, supported by a bootstrap value of 76%, gives rise to the hypothesis that the virus evolved independently since its introduction to wild rabbit populations on the Iberian Peninsula, with the Pyrenees acting as a natural barrier to rabbit and hence to virus dispersal. No differences were observed in RHDV sequences obtained from geographic regions where the rabbit subspecies O. c. algirus prevails compared with those obtained from O. c. cuniculus.

Original languageEnglish
Pages (from-to)368-373
Number of pages6
JournalVeterinary Microbiology
Volume135
Issue number3-4
DOIs
Publication statusPublished - Mar 30 2009

Fingerprint

Rabbit Haemorrhagic Disease Virus
Rabbit hemorrhagic disease virus
Oryctolagus cuniculus
Iberian Peninsula
rabbits
Rabbits
Portugal
Spain
viruses
Genotype
Viruses
Endemic Diseases
Geographic Locations
sampling
France
industry
Industry
nucleotide sequences
phylogeny
Population

Keywords

  • Iberian Peninsula
  • Oryctolagus cuniculus
  • Phylogeny
  • RHDV
  • Wild rabbit

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

Muller, A., Freitas, J., Silva, E., Le Gall-Reculé, G., Zwingelstein, F., Abrantes, J., ... Thompson, G. (2009). Evolution of rabbit haemorrhagic disease virus (RHDV) in the European rabbit (Oryctolagus cuniculus) from the Iberian Peninsula. Veterinary Microbiology, 135(3-4), 368-373. https://doi.org/10.1016/j.vetmic.2008.09.057

Evolution of rabbit haemorrhagic disease virus (RHDV) in the European rabbit (Oryctolagus cuniculus) from the Iberian Peninsula. / Muller, A.; Freitas, J.; Silva, E.; Le Gall-Reculé, G.; Zwingelstein, F.; Abrantes, J.; Esteves, P. J.; Alves, P. C.; van der Loo, W.; Kolodziejek, J.; Nowotny, N.; Thompson, G.

In: Veterinary Microbiology, Vol. 135, No. 3-4, 30.03.2009, p. 368-373.

Research output: Contribution to journalArticle

Muller, A, Freitas, J, Silva, E, Le Gall-Reculé, G, Zwingelstein, F, Abrantes, J, Esteves, PJ, Alves, PC, van der Loo, W, Kolodziejek, J, Nowotny, N & Thompson, G 2009, 'Evolution of rabbit haemorrhagic disease virus (RHDV) in the European rabbit (Oryctolagus cuniculus) from the Iberian Peninsula', Veterinary Microbiology, vol. 135, no. 3-4, pp. 368-373. https://doi.org/10.1016/j.vetmic.2008.09.057
Muller, A. ; Freitas, J. ; Silva, E. ; Le Gall-Reculé, G. ; Zwingelstein, F. ; Abrantes, J. ; Esteves, P. J. ; Alves, P. C. ; van der Loo, W. ; Kolodziejek, J. ; Nowotny, N. ; Thompson, G. / Evolution of rabbit haemorrhagic disease virus (RHDV) in the European rabbit (Oryctolagus cuniculus) from the Iberian Peninsula. In: Veterinary Microbiology. 2009 ; Vol. 135, No. 3-4. pp. 368-373.
@article{56f6a9ea14c044eb9361e0d73d811c2e,
title = "Evolution of rabbit haemorrhagic disease virus (RHDV) in the European rabbit (Oryctolagus cuniculus) from the Iberian Peninsula",
abstract = "To date information on rabbit haemorrhagic disease virus (RHDV) in Spain and Portugal has been scarce, although the disease is endemic and continues to have a considerable impact on species conservation and hunting industry. We analysed RHDVs obtained between 1994 and 2007 at different geographic locations in Portugal (40 samples), Spain (3 samples) and France (4 samples) from wild European rabbits (Oryctolagus cuniculus) that succumbed to the disease. Phylogenetic analyses based on partial VP60 gene sequences allowed a grouping of these RHDVs into three groups, termed {"}Iberian{"} Groups IB1, IB2 and IB3. Interestingly, these three Iberian groups clustered separately, though not far from earlier RHDVs of Genogroup 1 (containing e.g., strain {"}AST89{"}), but clearly distinct from globally described RHDV strains of Genogroups 2-6. This result, supported by a bootstrap value of 76{\%}, gives rise to the hypothesis that the virus evolved independently since its introduction to wild rabbit populations on the Iberian Peninsula, with the Pyrenees acting as a natural barrier to rabbit and hence to virus dispersal. No differences were observed in RHDV sequences obtained from geographic regions where the rabbit subspecies O. c. algirus prevails compared with those obtained from O. c. cuniculus.",
keywords = "Iberian Peninsula, Oryctolagus cuniculus, Phylogeny, RHDV, Wild rabbit",
author = "A. Muller and J. Freitas and E. Silva and {Le Gall-Recul{\'e}}, G. and F. Zwingelstein and J. Abrantes and Esteves, {P. J.} and Alves, {P. C.} and {van der Loo}, W. and J. Kolodziejek and N. Nowotny and G. Thompson",
year = "2009",
month = "3",
day = "30",
doi = "10.1016/j.vetmic.2008.09.057",
language = "English",
volume = "135",
pages = "368--373",
journal = "Veterinary Microbiology",
issn = "0378-1135",
publisher = "Elsevier",
number = "3-4",

}

TY - JOUR

T1 - Evolution of rabbit haemorrhagic disease virus (RHDV) in the European rabbit (Oryctolagus cuniculus) from the Iberian Peninsula

AU - Muller, A.

AU - Freitas, J.

AU - Silva, E.

AU - Le Gall-Reculé, G.

AU - Zwingelstein, F.

AU - Abrantes, J.

AU - Esteves, P. J.

AU - Alves, P. C.

AU - van der Loo, W.

AU - Kolodziejek, J.

AU - Nowotny, N.

AU - Thompson, G.

PY - 2009/3/30

Y1 - 2009/3/30

N2 - To date information on rabbit haemorrhagic disease virus (RHDV) in Spain and Portugal has been scarce, although the disease is endemic and continues to have a considerable impact on species conservation and hunting industry. We analysed RHDVs obtained between 1994 and 2007 at different geographic locations in Portugal (40 samples), Spain (3 samples) and France (4 samples) from wild European rabbits (Oryctolagus cuniculus) that succumbed to the disease. Phylogenetic analyses based on partial VP60 gene sequences allowed a grouping of these RHDVs into three groups, termed "Iberian" Groups IB1, IB2 and IB3. Interestingly, these three Iberian groups clustered separately, though not far from earlier RHDVs of Genogroup 1 (containing e.g., strain "AST89"), but clearly distinct from globally described RHDV strains of Genogroups 2-6. This result, supported by a bootstrap value of 76%, gives rise to the hypothesis that the virus evolved independently since its introduction to wild rabbit populations on the Iberian Peninsula, with the Pyrenees acting as a natural barrier to rabbit and hence to virus dispersal. No differences were observed in RHDV sequences obtained from geographic regions where the rabbit subspecies O. c. algirus prevails compared with those obtained from O. c. cuniculus.

AB - To date information on rabbit haemorrhagic disease virus (RHDV) in Spain and Portugal has been scarce, although the disease is endemic and continues to have a considerable impact on species conservation and hunting industry. We analysed RHDVs obtained between 1994 and 2007 at different geographic locations in Portugal (40 samples), Spain (3 samples) and France (4 samples) from wild European rabbits (Oryctolagus cuniculus) that succumbed to the disease. Phylogenetic analyses based on partial VP60 gene sequences allowed a grouping of these RHDVs into three groups, termed "Iberian" Groups IB1, IB2 and IB3. Interestingly, these three Iberian groups clustered separately, though not far from earlier RHDVs of Genogroup 1 (containing e.g., strain "AST89"), but clearly distinct from globally described RHDV strains of Genogroups 2-6. This result, supported by a bootstrap value of 76%, gives rise to the hypothesis that the virus evolved independently since its introduction to wild rabbit populations on the Iberian Peninsula, with the Pyrenees acting as a natural barrier to rabbit and hence to virus dispersal. No differences were observed in RHDV sequences obtained from geographic regions where the rabbit subspecies O. c. algirus prevails compared with those obtained from O. c. cuniculus.

KW - Iberian Peninsula

KW - Oryctolagus cuniculus

KW - Phylogeny

KW - RHDV

KW - Wild rabbit

UR - http://www.scopus.com/inward/record.url?scp=62149127401&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=62149127401&partnerID=8YFLogxK

U2 - 10.1016/j.vetmic.2008.09.057

DO - 10.1016/j.vetmic.2008.09.057

M3 - Article

C2 - 18977620

AN - SCOPUS:62149127401

VL - 135

SP - 368

EP - 373

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

IS - 3-4

ER -