Evaluation of the pulmonary effects of short-term nose-only cigarette smoke exposure in mice

Abderrahim Nemmar, Haider Raza, Deepa Subramaniyan, Annie John, Mohamed Elwasila, Badreldin H. Ali, Ernest Adeghate

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Much is known about the chronic effects of cigarette smoke (CS) on lung function and inflammation and development of chronic obstructive pulmonary disease. However, the underlying pathophysiological mechanisms related to the short-term exposure to CS are not fully understood. Here, we assessed the effect of CS generated by nine consecutive cigarettes per day for four days in a nose-only exposure system on airway resistance measured using forced oscillation technique, lung inflammation and oxidative stress in BALB/c mice. Control mice were exposed to air. Mice exposed to CS showed a significant increase of neutrophils and lymphocytes numbers in bronchoalveolar lavage (BAL). The total protein and endothelin levels in BAL fluid were significantly augmented suggesting an increase of alveolar-capillary barrier permeability. Similarly, airway resistance was significantly increased in the CS group compared with controls. Furthermore, reactive oxygen species and lipid peroxidation levels in lung tissue were significantly increased. The antioxidant activities of reduced glutathione, glutathione S transferase and superoxide dismutase were all significantly increased following CS exposure, indicating that CS could trigger adaptive responses that counterbalance the potentially damaging activity of oxygen radicals induced by CS exposure. In conclusion, our data indicate that short-term nose-only exposure to CS causes lung inflammation and increase of airway resistance mediated at least partly through the oxidative stress.

Original languageEnglish
Pages (from-to)1449-1456
Number of pages8
JournalExperimental Biology and Medicine
Volume237
Issue number12
DOIs
Publication statusPublished - Dec 2012

Fingerprint

Nose
Smoke
Tobacco Products
Lung
Airway Resistance
Pneumonia
Oxidative stress
Reactive Oxygen Species
Oxidative Stress
Pulmonary diseases
Lymphocytes
Endothelins
Bronchoalveolar Lavage Fluid
Lymphocyte Count
Capillary Permeability
Bronchoalveolar Lavage
Glutathione Transferase
Chronic Obstructive Pulmonary Disease
Lipid Peroxidation
Superoxide Dismutase

Keywords

  • Airway resistance
  • Cigarette smoke
  • Nose-only exposure
  • Oxidative stress
  • Short-term exposure

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Evaluation of the pulmonary effects of short-term nose-only cigarette smoke exposure in mice. / Nemmar, Abderrahim; Raza, Haider; Subramaniyan, Deepa; John, Annie; Elwasila, Mohamed; Ali, Badreldin H.; Adeghate, Ernest.

In: Experimental Biology and Medicine, Vol. 237, No. 12, 12.2012, p. 1449-1456.

Research output: Contribution to journalArticle

Nemmar, A, Raza, H, Subramaniyan, D, John, A, Elwasila, M, Ali, BH & Adeghate, E 2012, 'Evaluation of the pulmonary effects of short-term nose-only cigarette smoke exposure in mice', Experimental Biology and Medicine, vol. 237, no. 12, pp. 1449-1456. https://doi.org/10.1258/ebm.2012.012103
Nemmar, Abderrahim ; Raza, Haider ; Subramaniyan, Deepa ; John, Annie ; Elwasila, Mohamed ; Ali, Badreldin H. ; Adeghate, Ernest. / Evaluation of the pulmonary effects of short-term nose-only cigarette smoke exposure in mice. In: Experimental Biology and Medicine. 2012 ; Vol. 237, No. 12. pp. 1449-1456.
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