Evaluation of a quadruplex short tandem repeat system (HUMVWA31/A, HUMD11S554, HUMAPOAI1, and HUMACTBP2 loci) for forensic identity testing, confident typing of complex alleles, and population databases

Madjid Delghandi, Berit Janne Branting, Leif Jørgensen

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Aim. To evaluate a quadruplex short tandem repeat (STR) system based on the amplification of allelic products from HUMVWA31/A, HUMD11S554, HUMACTBP2, and HUMAPOAI1 loci for routine identity testing in forensic casework, and to provide details for the construction of a "multiplex allelic ladder" for confident typing of complex alleles from the quadruplex STR system and compiling a database for three representative populations living in Northern Norway (Norwegian, Saami, and Russian/Ukrainian). Methods. The constructed "multiplex allelic ladder" consisted of 44 regularly spaced and uniform alleles, spanning from 135 to 333 bp, and included 9 HUMVWA31/A alleles (TCTA repeat unit), 9 HUMD11S554 alleles (AAAG repeat unit), and 26 HUMACTBP2 alleles (AAAG repeat unit). Confident typing of selected complex alleles from the quadruplex STR system was secured only when they were compared with their matching ladder composed of the same repetitive unit as in our constructed "multiplex allelic ladder". Using different fluorescent dyes, PCR products from the quadruplex STR system were sized with constructed allelic ladder (red labeled) in one lane. Results. Population database was established for unrelated Norwegians (n=141), Saami (n=78), and Russians/Ukrainians (n=138). Combined paternity exclusions were 99.86%, 99.94%, 99.95% and the values of probability of match were 8.0x10-8, 1.4x10-8, and 1.1x10-8, in Saami, Norwegians, and Russians/Ukrainians, respectively. Conclusion. We conclude that by applying our constructed allelic ladder a confident typing of alleles from our quadruplex STR system may be ensured. Further, the high efficiency of the selected loci makes this system a reliable tool for use in forensic casework.

Original languageEnglish
Pages (from-to)33-44
Number of pages12
JournalCroatian Medical Journal
Volume42
Issue number1
Publication statusPublished - 2001

Fingerprint

Microsatellite Repeats
Alleles
Databases
Population
Norway
Fluorescent Dyes
Polymerase Chain Reaction

Keywords

  • Alleles
  • DNA
  • Forensic medicine
  • Genetics
  • Genotyping
  • Norway
  • Nucleic acid
  • Polymerase chain reaction
  • Population
  • Repetitive sequences
  • Tandem repeat sequences

ASJC Scopus subject areas

  • Medicine(all)

Cite this

@article{d67439ba9d3f4d9f9df65e8bbf579953,
title = "Evaluation of a quadruplex short tandem repeat system (HUMVWA31/A, HUMD11S554, HUMAPOAI1, and HUMACTBP2 loci) for forensic identity testing, confident typing of complex alleles, and population databases",
abstract = "Aim. To evaluate a quadruplex short tandem repeat (STR) system based on the amplification of allelic products from HUMVWA31/A, HUMD11S554, HUMACTBP2, and HUMAPOAI1 loci for routine identity testing in forensic casework, and to provide details for the construction of a {"}multiplex allelic ladder{"} for confident typing of complex alleles from the quadruplex STR system and compiling a database for three representative populations living in Northern Norway (Norwegian, Saami, and Russian/Ukrainian). Methods. The constructed {"}multiplex allelic ladder{"} consisted of 44 regularly spaced and uniform alleles, spanning from 135 to 333 bp, and included 9 HUMVWA31/A alleles (TCTA repeat unit), 9 HUMD11S554 alleles (AAAG repeat unit), and 26 HUMACTBP2 alleles (AAAG repeat unit). Confident typing of selected complex alleles from the quadruplex STR system was secured only when they were compared with their matching ladder composed of the same repetitive unit as in our constructed {"}multiplex allelic ladder{"}. Using different fluorescent dyes, PCR products from the quadruplex STR system were sized with constructed allelic ladder (red labeled) in one lane. Results. Population database was established for unrelated Norwegians (n=141), Saami (n=78), and Russians/Ukrainians (n=138). Combined paternity exclusions were 99.86{\%}, 99.94{\%}, 99.95{\%} and the values of probability of match were 8.0x10-8, 1.4x10-8, and 1.1x10-8, in Saami, Norwegians, and Russians/Ukrainians, respectively. Conclusion. We conclude that by applying our constructed allelic ladder a confident typing of alleles from our quadruplex STR system may be ensured. Further, the high efficiency of the selected loci makes this system a reliable tool for use in forensic casework.",
keywords = "Alleles, DNA, Forensic medicine, Genetics, Genotyping, Norway, Nucleic acid, Polymerase chain reaction, Population, Repetitive sequences, Tandem repeat sequences",
author = "Madjid Delghandi and Branting, {Berit Janne} and Leif J{\o}rgensen",
year = "2001",
language = "English",
volume = "42",
pages = "33--44",
journal = "Croatian Medical Journal",
issn = "0353-9504",
publisher = "Medicinska Naklada d.o.o",
number = "1",

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TY - JOUR

T1 - Evaluation of a quadruplex short tandem repeat system (HUMVWA31/A, HUMD11S554, HUMAPOAI1, and HUMACTBP2 loci) for forensic identity testing, confident typing of complex alleles, and population databases

AU - Delghandi, Madjid

AU - Branting, Berit Janne

AU - Jørgensen, Leif

PY - 2001

Y1 - 2001

N2 - Aim. To evaluate a quadruplex short tandem repeat (STR) system based on the amplification of allelic products from HUMVWA31/A, HUMD11S554, HUMACTBP2, and HUMAPOAI1 loci for routine identity testing in forensic casework, and to provide details for the construction of a "multiplex allelic ladder" for confident typing of complex alleles from the quadruplex STR system and compiling a database for three representative populations living in Northern Norway (Norwegian, Saami, and Russian/Ukrainian). Methods. The constructed "multiplex allelic ladder" consisted of 44 regularly spaced and uniform alleles, spanning from 135 to 333 bp, and included 9 HUMVWA31/A alleles (TCTA repeat unit), 9 HUMD11S554 alleles (AAAG repeat unit), and 26 HUMACTBP2 alleles (AAAG repeat unit). Confident typing of selected complex alleles from the quadruplex STR system was secured only when they were compared with their matching ladder composed of the same repetitive unit as in our constructed "multiplex allelic ladder". Using different fluorescent dyes, PCR products from the quadruplex STR system were sized with constructed allelic ladder (red labeled) in one lane. Results. Population database was established for unrelated Norwegians (n=141), Saami (n=78), and Russians/Ukrainians (n=138). Combined paternity exclusions were 99.86%, 99.94%, 99.95% and the values of probability of match were 8.0x10-8, 1.4x10-8, and 1.1x10-8, in Saami, Norwegians, and Russians/Ukrainians, respectively. Conclusion. We conclude that by applying our constructed allelic ladder a confident typing of alleles from our quadruplex STR system may be ensured. Further, the high efficiency of the selected loci makes this system a reliable tool for use in forensic casework.

AB - Aim. To evaluate a quadruplex short tandem repeat (STR) system based on the amplification of allelic products from HUMVWA31/A, HUMD11S554, HUMACTBP2, and HUMAPOAI1 loci for routine identity testing in forensic casework, and to provide details for the construction of a "multiplex allelic ladder" for confident typing of complex alleles from the quadruplex STR system and compiling a database for three representative populations living in Northern Norway (Norwegian, Saami, and Russian/Ukrainian). Methods. The constructed "multiplex allelic ladder" consisted of 44 regularly spaced and uniform alleles, spanning from 135 to 333 bp, and included 9 HUMVWA31/A alleles (TCTA repeat unit), 9 HUMD11S554 alleles (AAAG repeat unit), and 26 HUMACTBP2 alleles (AAAG repeat unit). Confident typing of selected complex alleles from the quadruplex STR system was secured only when they were compared with their matching ladder composed of the same repetitive unit as in our constructed "multiplex allelic ladder". Using different fluorescent dyes, PCR products from the quadruplex STR system were sized with constructed allelic ladder (red labeled) in one lane. Results. Population database was established for unrelated Norwegians (n=141), Saami (n=78), and Russians/Ukrainians (n=138). Combined paternity exclusions were 99.86%, 99.94%, 99.95% and the values of probability of match were 8.0x10-8, 1.4x10-8, and 1.1x10-8, in Saami, Norwegians, and Russians/Ukrainians, respectively. Conclusion. We conclude that by applying our constructed allelic ladder a confident typing of alleles from our quadruplex STR system may be ensured. Further, the high efficiency of the selected loci makes this system a reliable tool for use in forensic casework.

KW - Alleles

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KW - Genetics

KW - Genotyping

KW - Norway

KW - Nucleic acid

KW - Polymerase chain reaction

KW - Population

KW - Repetitive sequences

KW - Tandem repeat sequences

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