Estimation of numbers of malaria clones in blood samples

W. G. Hill, H. A. Babiker

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Methods are derived for estimating the mean number of clones of the haploid malaria parasite Plasmodium falciparum from samples of blood of infected hosts which have been tested for the presence of alleles at marker loci. For example, at a locus with three alleles the sample might contain only A 1, or A 1 and A 2, or A 1, A 2 and A 3, with multiple allele classes being more common at high infection rates. Assuming either a Poisson or negative binomial distribution of numbers of infections per host, formulae are derived for the frequency of different classes of blood samples, and maximum likelihood methods are used to estimate the mean number of clones and allele frequencies. Two data sets, each on two loci, are analysed. One data set was from the same locality in Tanzania from which oocysts of the parasite in mosquito vectors were tested for clonality (i.e. diploid unions of gametes from the same clone) using genetic markers. Good agreement was obtained between the observed clonality in oocysts and that expected from the number of infections per host (mean approximately three).

Original languageEnglish
Pages (from-to)249-257
Number of pages9
JournalProceedings of the Royal Society B: Biological Sciences
Volume262
Issue number1365
DOIs
Publication statusPublished - 1995

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

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