Escin attenuates behavioral impairments, oxidative stress and inflammation in a chronic MPTP/probenecid mouse model of Parkinson's disease

Govindasamy Pushpavathi Selvakumar, Udaiyappan Janakiraman, Musthafa Mohamed Essa, Arokiasamy Justin Thenmozhi, Thamilarasan Manivasagam

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Parkinson's disease (PD) is a progressive neurodegenerative disorder that results mainly due to the death of dopaminergic neurons in the substantia nigra (SN), and subsequently has an effect on one's motor function and coordination. The current investigation explored the neuroprotective potential of escin, a natural triterpene-saponin on chronic 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine/probenecid (MPTP/p) induced mouse model of PD. Administration of MPTP led to the depleted striatal dopamine content, impaired patterns of behavior, enhanced oxidative stress and diminished expression of tyrosine hydroxylase (TH), dopamine transporter (DAT) and vesicular monoamine transporter-2 (VMAT-2). The expressions of interleukin-6 and -10, glial fibrillary acidic protein (GFAP), ionized calcium-binding adaptor protein-1 (IBA-1), tumor necrosis factor-α (TNF-α) and inducible nitric oxide synthase (iNOS) in SN were also enhanced. Oral treatment of escin significantly attenuated MPTP/p induced dopaminergic markers depletion, physiological abnormalities, oxidative stress and inhibit neuroinflammatory cytokine expressions in SN. The result of our study confirmed that escin mediated its protection against experimental PD through its antioxidant and anti-inflammatory properties.

Original languageEnglish
Pages (from-to)23-36
Number of pages14
JournalBrain Research
Volume1585
DOIs
Publication statusPublished - Oct 17 2014

Fingerprint

Escin
Probenecid
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
Substantia Nigra
Parkinson Disease
Oxidative Stress
Inflammation
Vesicular Monoamine Transport Proteins
Corpus Striatum
Triterpenes
Dopamine Plasma Membrane Transport Proteins
Calcium-Binding Proteins
Dopaminergic Neurons
Glial Fibrillary Acidic Protein
Saponins
Tyrosine 3-Monooxygenase
Parkinsonian Disorders
Nitric Oxide Synthase Type II
Neurodegenerative Diseases
Interleukin-10

Keywords

  • Behavior
  • Dopaminergic markers
  • Escin
  • Experimental PD
  • Oxidative stress
  • Pro-inflammatory

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Developmental Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Escin attenuates behavioral impairments, oxidative stress and inflammation in a chronic MPTP/probenecid mouse model of Parkinson's disease. / Selvakumar, Govindasamy Pushpavathi; Janakiraman, Udaiyappan; Essa, Musthafa Mohamed; Justin Thenmozhi, Arokiasamy; Manivasagam, Thamilarasan.

In: Brain Research, Vol. 1585, 17.10.2014, p. 23-36.

Research output: Contribution to journalArticle

Selvakumar, Govindasamy Pushpavathi ; Janakiraman, Udaiyappan ; Essa, Musthafa Mohamed ; Justin Thenmozhi, Arokiasamy ; Manivasagam, Thamilarasan. / Escin attenuates behavioral impairments, oxidative stress and inflammation in a chronic MPTP/probenecid mouse model of Parkinson's disease. In: Brain Research. 2014 ; Vol. 1585. pp. 23-36.
@article{8eeea160ec634e39964088b2c26d447d,
title = "Escin attenuates behavioral impairments, oxidative stress and inflammation in a chronic MPTP/probenecid mouse model of Parkinson's disease",
abstract = "Parkinson's disease (PD) is a progressive neurodegenerative disorder that results mainly due to the death of dopaminergic neurons in the substantia nigra (SN), and subsequently has an effect on one's motor function and coordination. The current investigation explored the neuroprotective potential of escin, a natural triterpene-saponin on chronic 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine/probenecid (MPTP/p) induced mouse model of PD. Administration of MPTP led to the depleted striatal dopamine content, impaired patterns of behavior, enhanced oxidative stress and diminished expression of tyrosine hydroxylase (TH), dopamine transporter (DAT) and vesicular monoamine transporter-2 (VMAT-2). The expressions of interleukin-6 and -10, glial fibrillary acidic protein (GFAP), ionized calcium-binding adaptor protein-1 (IBA-1), tumor necrosis factor-α (TNF-α) and inducible nitric oxide synthase (iNOS) in SN were also enhanced. Oral treatment of escin significantly attenuated MPTP/p induced dopaminergic markers depletion, physiological abnormalities, oxidative stress and inhibit neuroinflammatory cytokine expressions in SN. The result of our study confirmed that escin mediated its protection against experimental PD through its antioxidant and anti-inflammatory properties.",
keywords = "Behavior, Dopaminergic markers, Escin, Experimental PD, Oxidative stress, Pro-inflammatory",
author = "Selvakumar, {Govindasamy Pushpavathi} and Udaiyappan Janakiraman and Essa, {Musthafa Mohamed} and {Justin Thenmozhi}, Arokiasamy and Thamilarasan Manivasagam",
year = "2014",
month = "10",
day = "17",
doi = "10.1016/j.brainres.2014.03.010",
language = "English",
volume = "1585",
pages = "23--36",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",

}

TY - JOUR

T1 - Escin attenuates behavioral impairments, oxidative stress and inflammation in a chronic MPTP/probenecid mouse model of Parkinson's disease

AU - Selvakumar, Govindasamy Pushpavathi

AU - Janakiraman, Udaiyappan

AU - Essa, Musthafa Mohamed

AU - Justin Thenmozhi, Arokiasamy

AU - Manivasagam, Thamilarasan

PY - 2014/10/17

Y1 - 2014/10/17

N2 - Parkinson's disease (PD) is a progressive neurodegenerative disorder that results mainly due to the death of dopaminergic neurons in the substantia nigra (SN), and subsequently has an effect on one's motor function and coordination. The current investigation explored the neuroprotective potential of escin, a natural triterpene-saponin on chronic 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine/probenecid (MPTP/p) induced mouse model of PD. Administration of MPTP led to the depleted striatal dopamine content, impaired patterns of behavior, enhanced oxidative stress and diminished expression of tyrosine hydroxylase (TH), dopamine transporter (DAT) and vesicular monoamine transporter-2 (VMAT-2). The expressions of interleukin-6 and -10, glial fibrillary acidic protein (GFAP), ionized calcium-binding adaptor protein-1 (IBA-1), tumor necrosis factor-α (TNF-α) and inducible nitric oxide synthase (iNOS) in SN were also enhanced. Oral treatment of escin significantly attenuated MPTP/p induced dopaminergic markers depletion, physiological abnormalities, oxidative stress and inhibit neuroinflammatory cytokine expressions in SN. The result of our study confirmed that escin mediated its protection against experimental PD through its antioxidant and anti-inflammatory properties.

AB - Parkinson's disease (PD) is a progressive neurodegenerative disorder that results mainly due to the death of dopaminergic neurons in the substantia nigra (SN), and subsequently has an effect on one's motor function and coordination. The current investigation explored the neuroprotective potential of escin, a natural triterpene-saponin on chronic 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine/probenecid (MPTP/p) induced mouse model of PD. Administration of MPTP led to the depleted striatal dopamine content, impaired patterns of behavior, enhanced oxidative stress and diminished expression of tyrosine hydroxylase (TH), dopamine transporter (DAT) and vesicular monoamine transporter-2 (VMAT-2). The expressions of interleukin-6 and -10, glial fibrillary acidic protein (GFAP), ionized calcium-binding adaptor protein-1 (IBA-1), tumor necrosis factor-α (TNF-α) and inducible nitric oxide synthase (iNOS) in SN were also enhanced. Oral treatment of escin significantly attenuated MPTP/p induced dopaminergic markers depletion, physiological abnormalities, oxidative stress and inhibit neuroinflammatory cytokine expressions in SN. The result of our study confirmed that escin mediated its protection against experimental PD through its antioxidant and anti-inflammatory properties.

KW - Behavior

KW - Dopaminergic markers

KW - Escin

KW - Experimental PD

KW - Oxidative stress

KW - Pro-inflammatory

UR - http://www.scopus.com/inward/record.url?scp=84907629729&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84907629729&partnerID=8YFLogxK

U2 - 10.1016/j.brainres.2014.03.010

DO - 10.1016/j.brainres.2014.03.010

M3 - Article

VL - 1585

SP - 23

EP - 36

JO - Brain Research

JF - Brain Research

SN - 0006-8993

ER -