Escherichia coli tetracycline efflux determinants in relation to tetracycline residues in chicken

Al Bahry SN, Al Mashani BM, Al Ansari AS, Elshafie AE, Mahmoud IY

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objective: To screen for Escherichia coli (E. coli) resistant to tetracycline, followed by identification of tet efflux genes by polymerase chain reaction (PCR). In addition, detection of tetracycline residues in chicken livers and kidneys were conducted using high performance liquid chromatography-tandem quadrupole mass spectrometry (HPLC-MS-MS). Methods: Strains of E. coli were isolated from samples of chicken colon and screened for tetracycline resistance. Tetracycline genes conferring resistance (Tcr) were detected by polymerase chain reaction (PCR). Most of the isolates were resistant to tetracycline (97.9%). Results: PCR analysis indicated that Tcr E. coli R-plasmids contained tet(A), tet(B) and a combination of both efflux genes. None of the isolates contained other efflux tet genes tet (C, D, E and Y). High performance liquid chromatography-tandem quadrupole mass spectrometry (HPLC-MS-MS), a sensitive technique, was used to detect residues of chlortetracycline (CTC), oxytetracycline (OTC), doxycycline (DC) in chicken livers and kidneys. The samples containing tetracycline residues were at 0.13-0.65 pg/μL levels. Conclusions: Tetracycline and other antibiotics are commonly used in the poultry and meat production industry for prevention of microbial infections. Multiple antibiotic resistant bacteria in Oman have increased to alarming levels, threatening public health, domestic and may have adverse effect on environment.

Original languageEnglish
Pages (from-to)718-722
Number of pages5
JournalAsian Pacific Journal of Tropical Medicine
Volume6
Issue number9
DOIs
Publication statusPublished - Sep 2013

Fingerprint

Tetracycline
Chickens
Escherichia coli
High Pressure Liquid Chromatography
Genes
Tandem Mass Spectrometry
Polymerase Chain Reaction
Oman
Anti-Bacterial Agents
Kidney
Tetracycline Resistance
Chlortetracycline
Oxytetracycline
R Factors
Doxycycline
Liver
Poultry
Meat
Industry
Colon

Keywords

  • Antibiotic resistance genes
  • Chicken
  • Escherichia coli
  • Tetracycline

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Escherichia coli tetracycline efflux determinants in relation to tetracycline residues in chicken. / SN, Al Bahry; BM, Al Mashani; AS, Al Ansari; AE, Elshafie; IY, Mahmoud.

In: Asian Pacific Journal of Tropical Medicine, Vol. 6, No. 9, 09.2013, p. 718-722.

Research output: Contribution to journalArticle

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abstract = "Objective: To screen for Escherichia coli (E. coli) resistant to tetracycline, followed by identification of tet efflux genes by polymerase chain reaction (PCR). In addition, detection of tetracycline residues in chicken livers and kidneys were conducted using high performance liquid chromatography-tandem quadrupole mass spectrometry (HPLC-MS-MS). Methods: Strains of E. coli were isolated from samples of chicken colon and screened for tetracycline resistance. Tetracycline genes conferring resistance (Tcr) were detected by polymerase chain reaction (PCR). Most of the isolates were resistant to tetracycline (97.9{\%}). Results: PCR analysis indicated that Tcr E. coli R-plasmids contained tet(A), tet(B) and a combination of both efflux genes. None of the isolates contained other efflux tet genes tet (C, D, E and Y). High performance liquid chromatography-tandem quadrupole mass spectrometry (HPLC-MS-MS), a sensitive technique, was used to detect residues of chlortetracycline (CTC), oxytetracycline (OTC), doxycycline (DC) in chicken livers and kidneys. The samples containing tetracycline residues were at 0.13-0.65 pg/μL levels. Conclusions: Tetracycline and other antibiotics are commonly used in the poultry and meat production industry for prevention of microbial infections. Multiple antibiotic resistant bacteria in Oman have increased to alarming levels, threatening public health, domestic and may have adverse effect on environment.",
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