Equine herpes virus type 1 (EHV-1) infection induces alterations in the cytoskeleton of Vero cells but not apoptosis

I. Walter, N. Nowotny

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Summary. Effects of infection with two different strains of equine herpes virus type 1 (EHV-1; Piber 178/83, Kentucky D) on the cytoskeleton of Vero cells were investigated immunohistochemically, and evaluated by confocal laser scanning microscopy. Twenty four hours POSt EHV-1 infection the assembly of the microtubulus system of Vero cells was heavily disturbed. The Golgi region was dispersed into vesicles spread throughout the cytoplasm as demonstrated by WGA lectin binding. Other cytoskeletal elements such as cytokeratin, vimentin, and filamentous actin (F-actin) were not affected by EHV-1 infection. The nature of Vero cell death after EHV-1 infection was investigated by three different methods to include all possible stages of apoptosis. All methods failed to demonstrate characteristic apoptotic features, therefore, it seems likely that necrosis is the predominant way of cell death in EHV-1 infected Vero cells.

Original languageEnglish
Pages (from-to)1827-1836
Number of pages10
JournalArchives of Virology
Volume144
Issue number9
DOIs
Publication statusPublished - 1999

Fingerprint

Vero Cells
Virus Diseases
Cytoskeleton
Horses
Apoptosis
Viruses
Cell Death
Vimentin
Keratins
Lectins
Confocal Microscopy
Actins
Cytoplasm
Necrosis
Infection

ASJC Scopus subject areas

  • Genetics
  • Applied Microbiology and Biotechnology

Cite this

Equine herpes virus type 1 (EHV-1) infection induces alterations in the cytoskeleton of Vero cells but not apoptosis. / Walter, I.; Nowotny, N.

In: Archives of Virology, Vol. 144, No. 9, 1999, p. 1827-1836.

Research output: Contribution to journalArticle

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