Enhancement of the chemiluminescence of penicillamine and ephedrine after derivatization with aldehydes using tris(bipyridyl)ruthenium(II) peroxydisulfate system and its analytical application

Fakhr Eldin O Suliman, Mohammed M. Al-Hinai, Salma M Z Al-Kindy, Salama B. Salama

Research output: Contribution to journalArticle

20 Citations (Scopus)


A novel sequential injection (SI) method was developed for the determination of penicillamine (PA) and ephedrine (EP) based on the reaction of these drugs with tris(bipyridyl)ruthenium(II) (Ru(bpy)3 2+) and peroxydisulfate (S2O8 2-) in the presence of light. Derivatization of PA and EP with aldehydes has resulted in a significant enhancement of the chemiluminescence emission signal by at least 25 times for PA and 12 times for EP, leading to better sensitivities and lower detection limits for both drugs. The instrumental setup utilized a syringe pump and a multiposition valve to aspirate the reagents, (Ru(bpy)3 2+ and S2O8 2-), and a peristaltic pump to propel the sample. The experimental conditions affecting the derivatization reaction and the chemiluminescence reaction were systematically optimized using the univariate approach. Under the optimum conditions linear calibration curves between 0.2-24 μg mL-1 for PA and 0.2-20 μg mL-1 for EP were obtained. The detection limits were 0.1 μg mL-1 for PA and 0.03 μg mL-1 for EP. The procedure was applied to the analysis of PA and EP in pharmaceutical products and was found to be free from interferences from concomitants usually present in these preparations.

Original languageEnglish
Pages (from-to)1256-1264
Number of pages9
Issue number5
Publication statusPublished - Feb 15 2008



  • Chemiluminescence
  • Ephedrine
  • Penicillamine
  • Peroxydisulfate
  • Sequential injection
  • Tris(bipyridyl) ruthenium(II)

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

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