Enhancement of on chip chemiluminescence signal intensity of tris(1,10-phenanthroline)-ruthenium(II) peroxydisulphate system for analysis of chlorpheniramine maleate in pharmaceutical formulations

Haider A J Al Lawati, Fakhr Eldin O Suliman, Salma M Z Al Kindy, Ali M. Al-Lawati, Gouri B. Varma, Imad Eldin M Nour

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The effect of detection chip geometry on chemiluminescence (CL) signal intensity of tris(1,10-phenanthroline)-ruthenium(II) peroxydisulphate system for analysis of chlorpheniramine maleate (CPM) in pharmaceutical formulations was investigated. It was observed that the design of the detection chip is very crucial and can play an important role in enhancing the CL signal intensity in this system. The CL signal intensity was enhanced 250% when a teardrop micromixer chip was used, compared to the commonly used serpentine chip geometry. The study was conducted using a multi-chip device. In this device, chip 1 was used to prepare and pump the reagent mixture, whereas chip 3 was used for pumping the sample. The two chips were connected to the teardrop chip (2) via silica capillary where detection took place. Non-linear regression curve fitting of the calibration data revealed that the calibration curves are best described by third order polynomial equation with excellent correlation coefficients (R2 = 0.9998) for the concentration range 7.69 × 10-8 to 5.12 × 10-5 mol L-1. A linear response is also observed over the range 7.69 × 10-8 to 1.28 × 10-5 mol L-1 (R2 = 0.9996) and the detection limit was found to be 5.49 × 10-8 mol L-1. The device was successfully used for the analysis of CPM in tablets and a multi-component cough syrup. Results were reproducible with relative standard deviation (RSD) of 0.6-1.1%.

Original languageEnglish
Pages (from-to)1999-2002
Number of pages4
JournalTalanta
Volume82
Issue number5
DOIs
Publication statusPublished - Oct 15 2010

Fingerprint

Chlorpheniramine
Drug Compounding
Chemiluminescence
Systems Analysis
Luminescence
Equipment and Supplies
Calibration
Pharmaceutical Preparations
Geometry
Curve fitting
Cough
Silicon Dioxide
Tablets
Limit of Detection
Polynomials
Pumps
tris-(1,10-phenanthroline)ruthenium

Keywords

  • Ammonium peroxodisulfate
  • Chemiluminescence
  • Chlorpheniramine maleate
  • Microfluidics
  • Micromixer
  • Tris(1,10-phenanthroline)-ruthenium(II)

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

@article{97fc20ca84d343fe922c8ae91dc83028,
title = "Enhancement of on chip chemiluminescence signal intensity of tris(1,10-phenanthroline)-ruthenium(II) peroxydisulphate system for analysis of chlorpheniramine maleate in pharmaceutical formulations",
abstract = "The effect of detection chip geometry on chemiluminescence (CL) signal intensity of tris(1,10-phenanthroline)-ruthenium(II) peroxydisulphate system for analysis of chlorpheniramine maleate (CPM) in pharmaceutical formulations was investigated. It was observed that the design of the detection chip is very crucial and can play an important role in enhancing the CL signal intensity in this system. The CL signal intensity was enhanced 250{\%} when a teardrop micromixer chip was used, compared to the commonly used serpentine chip geometry. The study was conducted using a multi-chip device. In this device, chip 1 was used to prepare and pump the reagent mixture, whereas chip 3 was used for pumping the sample. The two chips were connected to the teardrop chip (2) via silica capillary where detection took place. Non-linear regression curve fitting of the calibration data revealed that the calibration curves are best described by third order polynomial equation with excellent correlation coefficients (R2 = 0.9998) for the concentration range 7.69 × 10-8 to 5.12 × 10-5 mol L-1. A linear response is also observed over the range 7.69 × 10-8 to 1.28 × 10-5 mol L-1 (R2 = 0.9996) and the detection limit was found to be 5.49 × 10-8 mol L-1. The device was successfully used for the analysis of CPM in tablets and a multi-component cough syrup. Results were reproducible with relative standard deviation (RSD) of 0.6-1.1{\%}.",
keywords = "Ammonium peroxodisulfate, Chemiluminescence, Chlorpheniramine maleate, Microfluidics, Micromixer, Tris(1,10-phenanthroline)-ruthenium(II)",
author = "{Al Lawati}, {Haider A J} and Suliman, {Fakhr Eldin O} and {Al Kindy}, {Salma M Z} and Al-Lawati, {Ali M.} and Varma, {Gouri B.} and Nour, {Imad Eldin M}",
year = "2010",
month = "10",
day = "15",
doi = "10.1016/j.talanta.2010.08.018",
language = "English",
volume = "82",
pages = "1999--2002",
journal = "Talanta",
issn = "0039-9140",
publisher = "Elsevier",
number = "5",

}

TY - JOUR

T1 - Enhancement of on chip chemiluminescence signal intensity of tris(1,10-phenanthroline)-ruthenium(II) peroxydisulphate system for analysis of chlorpheniramine maleate in pharmaceutical formulations

AU - Al Lawati, Haider A J

AU - Suliman, Fakhr Eldin O

AU - Al Kindy, Salma M Z

AU - Al-Lawati, Ali M.

AU - Varma, Gouri B.

AU - Nour, Imad Eldin M

PY - 2010/10/15

Y1 - 2010/10/15

N2 - The effect of detection chip geometry on chemiluminescence (CL) signal intensity of tris(1,10-phenanthroline)-ruthenium(II) peroxydisulphate system for analysis of chlorpheniramine maleate (CPM) in pharmaceutical formulations was investigated. It was observed that the design of the detection chip is very crucial and can play an important role in enhancing the CL signal intensity in this system. The CL signal intensity was enhanced 250% when a teardrop micromixer chip was used, compared to the commonly used serpentine chip geometry. The study was conducted using a multi-chip device. In this device, chip 1 was used to prepare and pump the reagent mixture, whereas chip 3 was used for pumping the sample. The two chips were connected to the teardrop chip (2) via silica capillary where detection took place. Non-linear regression curve fitting of the calibration data revealed that the calibration curves are best described by third order polynomial equation with excellent correlation coefficients (R2 = 0.9998) for the concentration range 7.69 × 10-8 to 5.12 × 10-5 mol L-1. A linear response is also observed over the range 7.69 × 10-8 to 1.28 × 10-5 mol L-1 (R2 = 0.9996) and the detection limit was found to be 5.49 × 10-8 mol L-1. The device was successfully used for the analysis of CPM in tablets and a multi-component cough syrup. Results were reproducible with relative standard deviation (RSD) of 0.6-1.1%.

AB - The effect of detection chip geometry on chemiluminescence (CL) signal intensity of tris(1,10-phenanthroline)-ruthenium(II) peroxydisulphate system for analysis of chlorpheniramine maleate (CPM) in pharmaceutical formulations was investigated. It was observed that the design of the detection chip is very crucial and can play an important role in enhancing the CL signal intensity in this system. The CL signal intensity was enhanced 250% when a teardrop micromixer chip was used, compared to the commonly used serpentine chip geometry. The study was conducted using a multi-chip device. In this device, chip 1 was used to prepare and pump the reagent mixture, whereas chip 3 was used for pumping the sample. The two chips were connected to the teardrop chip (2) via silica capillary where detection took place. Non-linear regression curve fitting of the calibration data revealed that the calibration curves are best described by third order polynomial equation with excellent correlation coefficients (R2 = 0.9998) for the concentration range 7.69 × 10-8 to 5.12 × 10-5 mol L-1. A linear response is also observed over the range 7.69 × 10-8 to 1.28 × 10-5 mol L-1 (R2 = 0.9996) and the detection limit was found to be 5.49 × 10-8 mol L-1. The device was successfully used for the analysis of CPM in tablets and a multi-component cough syrup. Results were reproducible with relative standard deviation (RSD) of 0.6-1.1%.

KW - Ammonium peroxodisulfate

KW - Chemiluminescence

KW - Chlorpheniramine maleate

KW - Microfluidics

KW - Micromixer

KW - Tris(1,10-phenanthroline)-ruthenium(II)

UR - http://www.scopus.com/inward/record.url?scp=77957346223&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77957346223&partnerID=8YFLogxK

U2 - 10.1016/j.talanta.2010.08.018

DO - 10.1016/j.talanta.2010.08.018

M3 - Article

VL - 82

SP - 1999

EP - 2002

JO - Talanta

JF - Talanta

SN - 0039-9140

IS - 5

ER -