TY - JOUR
T1 - Enhancement of on chip chemiluminescence signal intensity of tris(1,10-phenanthroline)-ruthenium(II) peroxydisulphate system for analysis of chlorpheniramine maleate in pharmaceutical formulations
AU - Al Lawati, Haider A.J.
AU - Suliman, Fakhr Eldin O.
AU - Al Kindy, Salma M.Z.
AU - Al-Lawati, Ali M.
AU - Varma, Gouri B.
AU - Nour, Imad Eldin M.
N1 - Funding Information:
The project was funded by H.M. grant (SR/SCI/CHEM/09/01) Sultan Qaboos University. The authors thank both Central Laboratory of Drug analysis – Ministry of Health, Sultanate of Oman and National Pharmaceutical Industries (NPI) – Sultanate of Oman.
PY - 2010/10/15
Y1 - 2010/10/15
N2 - The effect of detection chip geometry on chemiluminescence (CL) signal intensity of tris(1,10-phenanthroline)-ruthenium(II) peroxydisulphate system for analysis of chlorpheniramine maleate (CPM) in pharmaceutical formulations was investigated. It was observed that the design of the detection chip is very crucial and can play an important role in enhancing the CL signal intensity in this system. The CL signal intensity was enhanced 250% when a teardrop micromixer chip was used, compared to the commonly used serpentine chip geometry. The study was conducted using a multi-chip device. In this device, chip 1 was used to prepare and pump the reagent mixture, whereas chip 3 was used for pumping the sample. The two chips were connected to the teardrop chip (2) via silica capillary where detection took place. Non-linear regression curve fitting of the calibration data revealed that the calibration curves are best described by third order polynomial equation with excellent correlation coefficients (R2 = 0.9998) for the concentration range 7.69 × 10-8 to 5.12 × 10-5 mol L-1. A linear response is also observed over the range 7.69 × 10-8 to 1.28 × 10-5 mol L-1 (R2 = 0.9996) and the detection limit was found to be 5.49 × 10-8 mol L-1. The device was successfully used for the analysis of CPM in tablets and a multi-component cough syrup. Results were reproducible with relative standard deviation (RSD) of 0.6-1.1%.
AB - The effect of detection chip geometry on chemiluminescence (CL) signal intensity of tris(1,10-phenanthroline)-ruthenium(II) peroxydisulphate system for analysis of chlorpheniramine maleate (CPM) in pharmaceutical formulations was investigated. It was observed that the design of the detection chip is very crucial and can play an important role in enhancing the CL signal intensity in this system. The CL signal intensity was enhanced 250% when a teardrop micromixer chip was used, compared to the commonly used serpentine chip geometry. The study was conducted using a multi-chip device. In this device, chip 1 was used to prepare and pump the reagent mixture, whereas chip 3 was used for pumping the sample. The two chips were connected to the teardrop chip (2) via silica capillary where detection took place. Non-linear regression curve fitting of the calibration data revealed that the calibration curves are best described by third order polynomial equation with excellent correlation coefficients (R2 = 0.9998) for the concentration range 7.69 × 10-8 to 5.12 × 10-5 mol L-1. A linear response is also observed over the range 7.69 × 10-8 to 1.28 × 10-5 mol L-1 (R2 = 0.9996) and the detection limit was found to be 5.49 × 10-8 mol L-1. The device was successfully used for the analysis of CPM in tablets and a multi-component cough syrup. Results were reproducible with relative standard deviation (RSD) of 0.6-1.1%.
KW - Ammonium peroxodisulfate
KW - Chemiluminescence
KW - Chlorpheniramine maleate
KW - Microfluidics
KW - Micromixer
KW - Tris(1,10-phenanthroline)-ruthenium(II)
UR - http://www.scopus.com/inward/record.url?scp=77957346223&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77957346223&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2010.08.018
DO - 10.1016/j.talanta.2010.08.018
M3 - Article
C2 - 20875608
AN - SCOPUS:77957346223
SN - 0039-9140
VL - 82
SP - 1999
EP - 2002
JO - Talanta
JF - Talanta
IS - 5
ER -