Enhanced resistance to sheath blight by constitutive expression of infection-related rice chitinase in transgenic elite indica rice cultivars

Karabi Datta, Jumin Tu, Norman Oliva, Isabelita Ona, Rethinasamy Velazhahan, Twng Wah Mew, Subbaratnam Muthukrishnan, Swapan K. Datta

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148 Citations (Scopus)


Genetic transformation has been attempted for management of rice sheath blight disease, caused by Rhizoctonia solani. We introduced a PR-3 rice chitinase gene (RC7), isolated from R. solani-infected rice plants, into indica rice cultivars IR72, IR64, IR68899B, MH63, and Chinsurah Boro II by the biolistic and PEG-mediated transformation system. Inheritance was studied up to the T2 generation by Southern blot analysis. Western blot analysis of transgenic plants with polyclonal antibody revealed the presence of chitinase protein with a molecular weight of 35 kDa that reacts with chitinase antibody. The transformants synthesized different levels of chitinase proteins constitutively and progeny from the plants containing the chitinase gene showed different levels of enhanced resistance when challenged with the sheath blight pathogen R. solani.

Original languageEnglish
Pages (from-to)405-414
Number of pages10
JournalPlant Science
Issue number3
Publication statusPublished - Feb 5 2001



  • Chitinase
  • Enhanced resistance
  • Sheath blight
  • Transgenic rice

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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