Enhanced resistance to sheath blight by constitutive expression of infection-related rice chitinase in transgenic elite indica rice cultivars

Karabi Datta; Jumin Tu; Norman Oliva; Isabelita Ona; Rethinasamy Velazhahan; Twng Wah Mew; Subbaratnam Muthukrishnan; Swapan K. Datta

doi:10.1016/S0168-9452(00)00413-1

Enhanced resistance to sheath blight by constitutive expression of infection-related rice chitinase in transgenic elite indica rice cultivars

Karabi Datta, Jumin Tu, Norman Oliva, Isabelita Ona, Rethinasamy Velazhahan, Twng Wah Mew, Subbaratnam Muthukrishnan, Swapan K. Datta^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

190 Citations (Scopus)

Abstract

Genetic transformation has been attempted for management of rice sheath blight disease, caused by Rhizoctonia solani. We introduced a PR-3 rice chitinase gene (RC7), isolated from R. solani-infected rice plants, into indica rice cultivars IR72, IR64, IR68899B, MH63, and Chinsurah Boro II by the biolistic and PEG-mediated transformation system. Inheritance was studied up to the T₂ generation by Southern blot analysis. Western blot analysis of transgenic plants with polyclonal antibody revealed the presence of chitinase protein with a molecular weight of 35 kDa that reacts with chitinase antibody. The transformants synthesized different levels of chitinase proteins constitutively and progeny from the plants containing the chitinase gene showed different levels of enhanced resistance when challenged with the sheath blight pathogen R. solani.

Original language	English
Pages (from-to)	405-414
Number of pages	10
Journal	Plant Science
Volume	160
Issue number	3
DOIs	https://doi.org/10.1016/S0168-9452(00)00413-1
Publication status	Published - Feb 5 2001
Externally published	Yes

Keywords

Chitinase
Enhanced resistance
Sheath blight
Transgenic rice

ASJC Scopus subject areas

Genetics
Agronomy and Crop Science
Plant Science

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10.1016/S0168-9452(00)00413-1

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title = "Enhanced resistance to sheath blight by constitutive expression of infection-related rice chitinase in transgenic elite indica rice cultivars",

abstract = "Genetic transformation has been attempted for management of rice sheath blight disease, caused by Rhizoctonia solani. We introduced a PR-3 rice chitinase gene (RC7), isolated from R. solani-infected rice plants, into indica rice cultivars IR72, IR64, IR68899B, MH63, and Chinsurah Boro II by the biolistic and PEG-mediated transformation system. Inheritance was studied up to the T2 generation by Southern blot analysis. Western blot analysis of transgenic plants with polyclonal antibody revealed the presence of chitinase protein with a molecular weight of 35 kDa that reacts with chitinase antibody. The transformants synthesized different levels of chitinase proteins constitutively and progeny from the plants containing the chitinase gene showed different levels of enhanced resistance when challenged with the sheath blight pathogen R. solani.",

keywords = "Chitinase, Enhanced resistance, Sheath blight, Transgenic rice",

author = "Karabi Datta and Jumin Tu and Norman Oliva and Isabelita Ona and Rethinasamy Velazhahan and Mew, {Twng Wah} and Subbaratnam Muthukrishnan and Datta, {Swapan K.}",

note = "Funding Information: We thank BMZ (GTZ, Germany) and the Rockefeller Foundation, USA, for financial assistance. Thanks are due to Bill Hardy for editorial assistance.",

year = "2001",

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doi = "10.1016/S0168-9452(00)00413-1",

language = "English",

volume = "160",

pages = "405--414",

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T1 - Enhanced resistance to sheath blight by constitutive expression of infection-related rice chitinase in transgenic elite indica rice cultivars

AU - Datta, Karabi

AU - Tu, Jumin

AU - Oliva, Norman

AU - Ona, Isabelita

AU - Velazhahan, Rethinasamy

AU - Mew, Twng Wah

AU - Muthukrishnan, Subbaratnam

AU - Datta, Swapan K.

N1 - Funding Information: We thank BMZ (GTZ, Germany) and the Rockefeller Foundation, USA, for financial assistance. Thanks are due to Bill Hardy for editorial assistance.

PY - 2001/2/5

Y1 - 2001/2/5

N2 - Genetic transformation has been attempted for management of rice sheath blight disease, caused by Rhizoctonia solani. We introduced a PR-3 rice chitinase gene (RC7), isolated from R. solani-infected rice plants, into indica rice cultivars IR72, IR64, IR68899B, MH63, and Chinsurah Boro II by the biolistic and PEG-mediated transformation system. Inheritance was studied up to the T2 generation by Southern blot analysis. Western blot analysis of transgenic plants with polyclonal antibody revealed the presence of chitinase protein with a molecular weight of 35 kDa that reacts with chitinase antibody. The transformants synthesized different levels of chitinase proteins constitutively and progeny from the plants containing the chitinase gene showed different levels of enhanced resistance when challenged with the sheath blight pathogen R. solani.

AB - Genetic transformation has been attempted for management of rice sheath blight disease, caused by Rhizoctonia solani. We introduced a PR-3 rice chitinase gene (RC7), isolated from R. solani-infected rice plants, into indica rice cultivars IR72, IR64, IR68899B, MH63, and Chinsurah Boro II by the biolistic and PEG-mediated transformation system. Inheritance was studied up to the T2 generation by Southern blot analysis. Western blot analysis of transgenic plants with polyclonal antibody revealed the presence of chitinase protein with a molecular weight of 35 kDa that reacts with chitinase antibody. The transformants synthesized different levels of chitinase proteins constitutively and progeny from the plants containing the chitinase gene showed different levels of enhanced resistance when challenged with the sheath blight pathogen R. solani.

KW - Chitinase

KW - Enhanced resistance

KW - Sheath blight

KW - Transgenic rice

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DO - 10.1016/S0168-9452(00)00413-1

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SN - 0168-9452

VL - 160

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JO - Plant Science

JF - Plant Science

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ER -

Enhanced resistance to sheath blight by constitutive expression of infection-related rice chitinase in transgenic elite indica rice cultivars

Abstract

Keywords

ASJC Scopus subject areas

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