Endothelial cells freshly isolated from resistance-sized pulmonary arteries possess a unique K+ current profile

Dayle S. Hogg, Sulayma Albarwani, Andrew R L Davies, Roland Z. Kozlowski

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We have, for the first time, developed a reliable method for freshly isolating viable endothelial cells from resistance-sized rat pulmonary arteries. The endothelial origin of these cells was confirmed using indirect immunofluorescence, utilizing fluorescently labeled low-density lipoprotein. Biophysical and pharmacological patch-clamp experiments conducted under quasiphysiological cationic gradients revealed that these cells had a mean resting membrane potential of ~ -38 mV and displayed a delayed-rectifying K+ current. Immunohistochemical staining of rat lung cross-sections revealed an abundance of K(V)1.5 channel protein in pulmonary endothelium. This is the first report of a delayed-rectifying K+ current in endothelial cells of resistance-sized pulmonary arteries. Since changes in membrane potential associated with K+ channel activity affect release of vasoactive substances from endothelial cells, this finding has important implications for understanding the mechanisms underlying control of pulmonary vascular tone.

Original languageEnglish
Pages (from-to)405-409
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume263
Issue number2
DOIs
Publication statusPublished - Sep 24 1999

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Endothelial cells
Pulmonary Artery
Endothelial Cells
Rats
Membrane Potentials
Lung
Membranes
Clamping devices
LDL Lipoproteins
Indirect Fluorescent Antibody Technique
Endothelium
Blood Vessels
Pharmacology
Staining and Labeling
Proteins
Experiments

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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Endothelial cells freshly isolated from resistance-sized pulmonary arteries possess a unique K+ current profile. / Hogg, Dayle S.; Albarwani, Sulayma; Davies, Andrew R L; Kozlowski, Roland Z.

In: Biochemical and Biophysical Research Communications, Vol. 263, No. 2, 24.09.1999, p. 405-409.

Research output: Contribution to journalArticle

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