Endothelial cells freshly isolated from resistance-sized pulmonary arteries possess a unique K+ current profile

Dayle S. Hogg, Sulayma Albarwani, Andrew R L Davies, Roland Z. Kozlowski

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Abstract

We have, for the first time, developed a reliable method for freshly isolating viable endothelial cells from resistance-sized rat pulmonary arteries. The endothelial origin of these cells was confirmed using indirect immunofluorescence, utilizing fluorescently labeled low-density lipoprotein. Biophysical and pharmacological patch-clamp experiments conducted under quasiphysiological cationic gradients revealed that these cells had a mean resting membrane potential of ~ -38 mV and displayed a delayed-rectifying K+ current. Immunohistochemical staining of rat lung cross-sections revealed an abundance of K(V)1.5 channel protein in pulmonary endothelium. This is the first report of a delayed-rectifying K+ current in endothelial cells of resistance-sized pulmonary arteries. Since changes in membrane potential associated with K+ channel activity affect release of vasoactive substances from endothelial cells, this finding has important implications for understanding the mechanisms underlying control of pulmonary vascular tone.

Original languageEnglish
Pages (from-to)405-409
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume263
Issue number2
DOIs
Publication statusPublished - Sep 24 1999

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ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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