TY - JOUR
T1 - Endophytic fungi from the medicinal plant Aloe dhufarensis Lavranos exhibit antagonistic potential against phytopathogenic fungi
AU - Al-Rashdi, Fatma Khuseib Hamed
AU - Al-Sadi, Abdullah Mohammed
AU - Al-Riyamy, Bahja Z.
AU - Maharachchikumbura, Sajeewa S.N.
AU - Al-Sabahi, Jamal Nasser
AU - Velazhahan, Rethinasamy
N1 - Funding Information:
We thank Dr. Khalid Al-Farsi and Dr. Annette Patzelt, Oman Botanic Garden for providing Aloe dhufarensis and Ms. Houda Khlafan Al-Ruqaishi, CAMS, SQU for helping in GC?MS analysis. This work was supported by the SQU research grants EG/AGR/CROP/16/01 and IG/AGR/ CROP/18/01.
Funding Information:
We thank Dr. Khalid Al-Farsi and Dr. Annette Patzelt, Oman Botanic Garden for providing Aloe dhufarensis and Ms. Houda Khlafan Al-Ruqaishi, CAMS, SQU for helping in GC–MS analysis. This work was supported by the SQU research grants EG/AGR/CROP/16/01 and IG/AGR/ CROP/18/01.
Publisher Copyright:
© 2020
PY - 2022/7/1
Y1 - 2022/7/1
N2 - Aloe dhufarensis Lavranos is an important medicinal plant, however, the endophytic microorganisms associated with this plant and their antagonistic activities are not known. In this study, endophytic fungi and bacteria were isolated from the leaf tissues of A. dhufarensis and their antagonistic activity against the plant pathogens Fusarium sp. and Cladosporium sp. were evaluated using a dual culture assay. A total of 3 fungal endophytes and 12 bacterial endophytes were isolated from the leaf tissue segments of A. dhufarensis. Among them, two endophytic fungal isolates designated as SQUCC-F2-2 and SQUCC-F3-1 exhibited inhibitory activity against the phytopathogenic fungi tested. In addition, scanning electron microscopy analysis showed that they induced shriveling, pit formation and disintegration of the hyphae of the phytopathogenic fungi. These two endophytic fungal isolates were identified as Sarocladium kiliense (SQUCC-F2-2) and Penicillium oxalicum (SQUCC-F3-1) on the basis of nuclear ribosomal DNA internal transcribed spacer (ITS) region. Analysis of the metabolites produced by these endophytes revealed the presence of fatty acids, fatty acid methyl esters, furfuryl alcohol, 1,2-diols and amide, which have been identified in the literature as antimicrobial compounds. P. oxalicum produced 7 compounds, of which 2-Furanmethanol, Dodecanoic acid and Tetradecanoic acid were dominant. S. kiliense produced 8 compounds with 2,3-Butanediol as the main constituent. This is the first report of endophytic fungi of A. dhufarensis with antagonistic activity against phytopathogenic fungi.
AB - Aloe dhufarensis Lavranos is an important medicinal plant, however, the endophytic microorganisms associated with this plant and their antagonistic activities are not known. In this study, endophytic fungi and bacteria were isolated from the leaf tissues of A. dhufarensis and their antagonistic activity against the plant pathogens Fusarium sp. and Cladosporium sp. were evaluated using a dual culture assay. A total of 3 fungal endophytes and 12 bacterial endophytes were isolated from the leaf tissue segments of A. dhufarensis. Among them, two endophytic fungal isolates designated as SQUCC-F2-2 and SQUCC-F3-1 exhibited inhibitory activity against the phytopathogenic fungi tested. In addition, scanning electron microscopy analysis showed that they induced shriveling, pit formation and disintegration of the hyphae of the phytopathogenic fungi. These two endophytic fungal isolates were identified as Sarocladium kiliense (SQUCC-F2-2) and Penicillium oxalicum (SQUCC-F3-1) on the basis of nuclear ribosomal DNA internal transcribed spacer (ITS) region. Analysis of the metabolites produced by these endophytes revealed the presence of fatty acids, fatty acid methyl esters, furfuryl alcohol, 1,2-diols and amide, which have been identified in the literature as antimicrobial compounds. P. oxalicum produced 7 compounds, of which 2-Furanmethanol, Dodecanoic acid and Tetradecanoic acid were dominant. S. kiliense produced 8 compounds with 2,3-Butanediol as the main constituent. This is the first report of endophytic fungi of A. dhufarensis with antagonistic activity against phytopathogenic fungi.
KW - Aloe dhufarensis
KW - Antifungal compounds
KW - Endophyte
KW - Gas chromatography-mass spectrometry
KW - Penicillium oxalicum
KW - Sarocladium kiliense
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U2 - 10.1016/j.sajb.2020.05.022
DO - 10.1016/j.sajb.2020.05.022
M3 - Article
AN - SCOPUS:85086427553
SN - 0254-6299
VL - 147
SP - 1078
EP - 1085
JO - South African Journal of Botany
JF - South African Journal of Botany
ER -