Embryo transfer induces a subclinical endometritis in recipient mares which can be prevented by treatment with non-steroid anti-inflammatory drugs

P. Koblischke, H. Kindahl, S. Budik, J. Aurich, F. Palm, I. Walter, J. Kolodziejek, N. Nowotny, H. O. Hoppen, C. Aurich

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

We tested the hypothesis that subclinical endometritis occurs after embryo transfer (ET) in the horse. Recipient mares were treated with meclofenamic acid (M) or flunixin meglumin (F) after ET or were left untreated (n = 9 per group). Embryos were re-collected 4 days after transfer. Endometrial biopsies were taken for histology and analysis of cyclooxygenase-2 (COX-2) by immunohistochemistry and for PCR. Bacteriological swabs were collected from the uterus and lavage fluid of donor and recipient mares. Progesterone and prostaglandin F release was analysed in recipient mares after ET. Four days after ET, four embryos were recovered from group M and three from group F and untreated mares, each. The number of polymorph nuclear neutrophils was reduced in treated mares (p <0.05). Expression of mRNA for inflammatory cytokines did not differ between groups. In group M, expression of endometrial prostaglandin-E-synthase was higher than in group F (p <0.05). Three out of nine control mares unterwent preterm luteolysis (p <0.05 vs. treatment groups), prostaglandin release (p <0.05) and the number of COX-2 positive cells (p <0.01) were significantly higher than in treated mares. Only few bacteriological swabs were positive. In conclusion, treatment of embryo recipient mares with non-steroid anti-inflammatory drugs inhibits the inflammatory response of the endometrium after ET. Meclofenamic acid may have advantages in comparison to flunixin meglumin due to a different influence on prostaglandin synthesis that may not result in inhibition of embryonic mobility.

Original languageEnglish
Pages (from-to)1147-1158
Number of pages12
JournalTheriogenology
Volume70
Issue number7
DOIs
Publication statusPublished - Oct 15 2008

Fingerprint

anti-inflammatory agents
Endometritis
Embryo Transfer
endometritis
embryo transfer
mares
Anti-Inflammatory Agents
Meclofenamic Acid
Pharmaceutical Preparations
Embryonic Structures
Cyclooxygenase 2
Prostaglandins
prostaglandins
flunixin
embryo (animal)
Luteolysis
prostaglandin synthase
Therapeutics
Dinoprost
Therapeutic Irrigation

Keywords

  • Embryo transfer
  • Horse
  • Recipient

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Embryo transfer induces a subclinical endometritis in recipient mares which can be prevented by treatment with non-steroid anti-inflammatory drugs. / Koblischke, P.; Kindahl, H.; Budik, S.; Aurich, J.; Palm, F.; Walter, I.; Kolodziejek, J.; Nowotny, N.; Hoppen, H. O.; Aurich, C.

In: Theriogenology, Vol. 70, No. 7, 15.10.2008, p. 1147-1158.

Research output: Contribution to journalArticle

Koblischke, P, Kindahl, H, Budik, S, Aurich, J, Palm, F, Walter, I, Kolodziejek, J, Nowotny, N, Hoppen, HO & Aurich, C 2008, 'Embryo transfer induces a subclinical endometritis in recipient mares which can be prevented by treatment with non-steroid anti-inflammatory drugs', Theriogenology, vol. 70, no. 7, pp. 1147-1158. https://doi.org/10.1016/j.theriogenology.2008.06.037
Koblischke, P. ; Kindahl, H. ; Budik, S. ; Aurich, J. ; Palm, F. ; Walter, I. ; Kolodziejek, J. ; Nowotny, N. ; Hoppen, H. O. ; Aurich, C. / Embryo transfer induces a subclinical endometritis in recipient mares which can be prevented by treatment with non-steroid anti-inflammatory drugs. In: Theriogenology. 2008 ; Vol. 70, No. 7. pp. 1147-1158.
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AU - Aurich, J.

AU - Palm, F.

AU - Walter, I.

AU - Kolodziejek, J.

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AU - Hoppen, H. O.

AU - Aurich, C.

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