Electrochemically reduced fullerene–graphene oxide interface for swift detection of Parkinsons disease biomarkers

Jahangir Ahmad Rather*, Emad A. Khudaish, Abdul Munam, Ahsanulhaq Qurashi, Palanisamy Kannan

*Corresponding author for this work

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We are reporting first-time synthesis of the novel water-soluble fullerene–graphene oxide (C60–GO) conjugate by 1,3-dipolar cycloaddition reaction between fullerene (C60) and azide functionalized graphene oxide (GO–N3). The synthesized fullerene–graphene oxide (C60–GO) conjugate was characterized by fourier transform infrared spectroscopy (FTIR), Ultraviolet–visible (UV–vis) spectroscopy, electrochemical and field emission electron microscopy (FESEM). The C60–GO conjugate was immobilized on surface of glassy carbon electrode (GCE) using surface bound diazonium salts as an impact strategy. The nitrophenyl modified GCE (GCE–Ph–NO2) was fabricated by electrochemical reduction of nitrophenyl diazonium salt (N2 +Cl–Ph–NO2). The GCE–Ph–NO2 modified electrode was reduced to phenylamine-modified electrode (GCE–Ph–NH2) by sodium borohydride/gold–polyaniline (NaBH4/Au–PANI) system. The surface phenylamine groups of GCE–Ph–NH2 were converted to diazonium groups (GCE–Ph–N2+Cl) which upon electrochemical reduction in an aqueous C60–GO conjugate solution causes successful immobilization of C60–GO conjugate to give phenyl modified fullerene–graphene oxide interface (C60–GO–Ph–GCE). The C60–GO–Ph–GCE interface upon electrochemical reduction in 1.0 M potassium hydroxide (KOH) solution produces highly conductive electrochemically reduced (ERC60–GO–Ph–GCE) interface. The electrocatalytic activity of developed (ERC60–GO–Ph–GCE) interface originates from the synergetic effects of electrochemically reduced fullerene (ERC60) and graphene oxide (ERGO) species that makes the conjugate (C60–GO) film highly conductive. The (ERC60–GO–Ph–GCE) interface was applied for determination of the homovanilic acid (HVA; a biomarker for Parkinsons disease) over a concentration range from 0.1–7.2 μM with a detection limit of 0.03 μM. The developed (ERC60–GO–Ph–GCE) interface shows good sensitivity that makes it suitable for detection of HVA in biological fluids (urine).

Original languageEnglish
Pages (from-to)672-684
Number of pages13
JournalSensors and Actuators, B: Chemical
Volume237
DOIs
Publication statusPublished - Dec 1 2016

Keywords

  • Diazonium salts
  • Electrografting
  • Fullerene–graphene oxide (C–GO)
  • Gold–polyaniline (Au–PANI) nanocomposite
  • Homovanilic acid
  • Parkisons disease biomarkers

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry
  • Electrical and Electronic Engineering

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