Effects of hydrogen sulphide on the isolated perfused rat heart

Afthab Hussain, Helen Maddock, Hajar Al-Rajaibi, Ray J. Carson

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objectives: Hydrogen sulphide has been identified as a gas signalling molecule in the body, and has previously been shown to have vasorelaxant properties. The aim of the study was to investigate the effects of sodium hydrosulphide (NaHS), a hydrogen sulphide donor, on heart rate (HR), left ventricular developed pressure (LVDP) and coronary flow (CF) in the isolated perfused rat heart. Methods: A Langendorff isolated heart preparation was used to investigate the effect of a dose range of sodium hydrosulphide, in the presence and absence of inhibitors, on heart rate, left ventricular developed pressure and coronary flow. Results: Sodium hydrosulphide caused a significant decrease in heart rate at a concentration of 10-3 M (P <0.001). This decrease was partially inhibited by glibenclamide, a KATP channel blocker (P <0.05); L-NAME, a nitric oxide synthase inhibitor (P <0.001), and methylene blue (P <0.001), but not by H-89, a protein kinase A inhibitor. Sodium hydrosulphide significantly increased coronary flow at concentrations of 10-4 - 10-3M (P <0.05). This response was significantly increased in the presence of L-NAME (P <0.001) and methylene blue (P <0.001), whereas H-89 inhibited the increase in coronary flow due to sodium hydrosulphide (P <0.001). Sodium hydrosulphide significantly decreased LVDP at all concentrations (P <0.001). In the presence of glibenclamide and H-89, the time period of the decrease in LVDP due to sodium hydrosulphide was extended (P <0.001), whereas methylene blue and L-NAME caused a significant reduction in the response to sodium hydrosulphide (P <0.05, P <0.01 respectively). Conclusion: Sodium hydrosulphide reduced heart rate and LVDP, and increased coronary flow in the isolated perfused rat heart; however, the mechanisms of action could not be fully elucidated.

Original languageEnglish
Pages (from-to)236-244
Number of pages9
JournalSultan Qaboos University Medical Journal
Volume11
Issue number2
Publication statusPublished - May 2011

Fingerprint

Hydrogen Sulfide
Ventricular Pressure
Methylene Blue
NG-Nitroarginine Methyl Ester
Heart Rate
Glyburide
sodium bisulfide
KATP Channels
Protein Kinase Inhibitors
Cyclic AMP-Dependent Protein Kinases
Vasodilator Agents
Nitric Oxide Synthase
Gases

Keywords

  • HS gasotransmitter
  • Heart
  • Hydrogen sulphide
  • Langendorff
  • Vasorelaxation

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Effects of hydrogen sulphide on the isolated perfused rat heart. / Hussain, Afthab; Maddock, Helen; Al-Rajaibi, Hajar; Carson, Ray J.

In: Sultan Qaboos University Medical Journal, Vol. 11, No. 2, 05.2011, p. 236-244.

Research output: Contribution to journalArticle

Hussain, Afthab ; Maddock, Helen ; Al-Rajaibi, Hajar ; Carson, Ray J. / Effects of hydrogen sulphide on the isolated perfused rat heart. In: Sultan Qaboos University Medical Journal. 2011 ; Vol. 11, No. 2. pp. 236-244.
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