Effects of cagA+ and cagA- strains of Helicobacter pylori on the human gastric mucus layer thickness

Mohammed S. Al-Marhoon, Sheila Nunn, Roger W. Soames

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Infection with cytotoxin-associated gene A (cagA) Helicobacter pylori is associated with severe gastric diseases, with contradictory views being expressed concerning the effect of H. pylori on the gastric mucus thickness. The aim of the present study was to differentiate between the effect of cagA+ and cagA- strains on gastric mucus thickness. Methods: Ninety-nine patients without peptic ulcers who were not on medication were randomly recruited from consecutive endoscopy clinics: six biopsies (five antral, one body) were obtained from each patient. Cryostat sections (18 μm) were cut and stained using the modified periodic acid-Schiff/ Alcian blue technique. Mucus thickness was measured using computer-assisted light microscopy. The H. pylori status was assessed by histology, Campylobacter-like organism (CLO)test and culture, and cagA+ status determined by polymerase chain reaction (PCR). Results: There was no significant difference (P = 0.784) in mean mucus thickness between cagA+ (52.7 ± 1.2 μm, n = 10), cagA- (46.6 ± 1.1 μm, n = 18) or H. pylori-negative patients (51.3 ± 1.1 μm, n = 30). In cagA- patients, mucus thickness was significantly reduced with increased H. pylori colonization density, Spearman (rs) = -0.805, P < 0.0001. In contrast, in cagA+ patients there was a weak positive, but not significant, association between mucus thickness and H. pylori colonization density, r s = 0.333, P = 0.381. Conclusions: The human gastric mucus thickness is not affected by infection with cagA+ or cagA- strains of H. pylori compared with uninfected. Although a trend of increased mucus thickness with cagA+ infection was observed.

Original languageEnglish
Pages (from-to)1246-1252
Number of pages7
JournalJournal of Gastroenterology and Hepatology (Australia)
Volume20
Issue number8
DOIs
Publication statusPublished - 2005

Fingerprint

Cytotoxins
Mucus
Helicobacter pylori
Stomach
Genes
Infection
Stomach Diseases
Alcian Blue
Periodic Acid
Campylobacter
Peptic Ulcer
Endoscopy
Microscopy
Histology
Biopsy
Light
Polymerase Chain Reaction

Keywords

  • Gastric
  • Helicobacter pylori
  • Mucus
  • Thickness

ASJC Scopus subject areas

  • Gastroenterology
  • Hepatology

Cite this

Effects of cagA+ and cagA- strains of Helicobacter pylori on the human gastric mucus layer thickness. / Al-Marhoon, Mohammed S.; Nunn, Sheila; Soames, Roger W.

In: Journal of Gastroenterology and Hepatology (Australia), Vol. 20, No. 8, 2005, p. 1246-1252.

Research output: Contribution to journalArticle

@article{6246507e14e34646961b335cac6a57ca,
title = "Effects of cagA+ and cagA- strains of Helicobacter pylori on the human gastric mucus layer thickness",
abstract = "Background: Infection with cytotoxin-associated gene A (cagA) Helicobacter pylori is associated with severe gastric diseases, with contradictory views being expressed concerning the effect of H. pylori on the gastric mucus thickness. The aim of the present study was to differentiate between the effect of cagA+ and cagA- strains on gastric mucus thickness. Methods: Ninety-nine patients without peptic ulcers who were not on medication were randomly recruited from consecutive endoscopy clinics: six biopsies (five antral, one body) were obtained from each patient. Cryostat sections (18 μm) were cut and stained using the modified periodic acid-Schiff/ Alcian blue technique. Mucus thickness was measured using computer-assisted light microscopy. The H. pylori status was assessed by histology, Campylobacter-like organism (CLO)test and culture, and cagA+ status determined by polymerase chain reaction (PCR). Results: There was no significant difference (P = 0.784) in mean mucus thickness between cagA+ (52.7 ± 1.2 μm, n = 10), cagA- (46.6 ± 1.1 μm, n = 18) or H. pylori-negative patients (51.3 ± 1.1 μm, n = 30). In cagA- patients, mucus thickness was significantly reduced with increased H. pylori colonization density, Spearman (rs) = -0.805, P < 0.0001. In contrast, in cagA+ patients there was a weak positive, but not significant, association between mucus thickness and H. pylori colonization density, r s = 0.333, P = 0.381. Conclusions: The human gastric mucus thickness is not affected by infection with cagA+ or cagA- strains of H. pylori compared with uninfected. Although a trend of increased mucus thickness with cagA+ infection was observed.",
keywords = "Gastric, Helicobacter pylori, Mucus, Thickness",
author = "Al-Marhoon, {Mohammed S.} and Sheila Nunn and Soames, {Roger W.}",
year = "2005",
doi = "10.1111/j.1440-1746.2005.03853.x",
language = "English",
volume = "20",
pages = "1246--1252",
journal = "Journal of Gastroenterology and Hepatology (Australia)",
issn = "0815-9319",
publisher = "Wiley-Blackwell",
number = "8",

}

TY - JOUR

T1 - Effects of cagA+ and cagA- strains of Helicobacter pylori on the human gastric mucus layer thickness

AU - Al-Marhoon, Mohammed S.

AU - Nunn, Sheila

AU - Soames, Roger W.

PY - 2005

Y1 - 2005

N2 - Background: Infection with cytotoxin-associated gene A (cagA) Helicobacter pylori is associated with severe gastric diseases, with contradictory views being expressed concerning the effect of H. pylori on the gastric mucus thickness. The aim of the present study was to differentiate between the effect of cagA+ and cagA- strains on gastric mucus thickness. Methods: Ninety-nine patients without peptic ulcers who were not on medication were randomly recruited from consecutive endoscopy clinics: six biopsies (five antral, one body) were obtained from each patient. Cryostat sections (18 μm) were cut and stained using the modified periodic acid-Schiff/ Alcian blue technique. Mucus thickness was measured using computer-assisted light microscopy. The H. pylori status was assessed by histology, Campylobacter-like organism (CLO)test and culture, and cagA+ status determined by polymerase chain reaction (PCR). Results: There was no significant difference (P = 0.784) in mean mucus thickness between cagA+ (52.7 ± 1.2 μm, n = 10), cagA- (46.6 ± 1.1 μm, n = 18) or H. pylori-negative patients (51.3 ± 1.1 μm, n = 30). In cagA- patients, mucus thickness was significantly reduced with increased H. pylori colonization density, Spearman (rs) = -0.805, P < 0.0001. In contrast, in cagA+ patients there was a weak positive, but not significant, association between mucus thickness and H. pylori colonization density, r s = 0.333, P = 0.381. Conclusions: The human gastric mucus thickness is not affected by infection with cagA+ or cagA- strains of H. pylori compared with uninfected. Although a trend of increased mucus thickness with cagA+ infection was observed.

AB - Background: Infection with cytotoxin-associated gene A (cagA) Helicobacter pylori is associated with severe gastric diseases, with contradictory views being expressed concerning the effect of H. pylori on the gastric mucus thickness. The aim of the present study was to differentiate between the effect of cagA+ and cagA- strains on gastric mucus thickness. Methods: Ninety-nine patients without peptic ulcers who were not on medication were randomly recruited from consecutive endoscopy clinics: six biopsies (five antral, one body) were obtained from each patient. Cryostat sections (18 μm) were cut and stained using the modified periodic acid-Schiff/ Alcian blue technique. Mucus thickness was measured using computer-assisted light microscopy. The H. pylori status was assessed by histology, Campylobacter-like organism (CLO)test and culture, and cagA+ status determined by polymerase chain reaction (PCR). Results: There was no significant difference (P = 0.784) in mean mucus thickness between cagA+ (52.7 ± 1.2 μm, n = 10), cagA- (46.6 ± 1.1 μm, n = 18) or H. pylori-negative patients (51.3 ± 1.1 μm, n = 30). In cagA- patients, mucus thickness was significantly reduced with increased H. pylori colonization density, Spearman (rs) = -0.805, P < 0.0001. In contrast, in cagA+ patients there was a weak positive, but not significant, association between mucus thickness and H. pylori colonization density, r s = 0.333, P = 0.381. Conclusions: The human gastric mucus thickness is not affected by infection with cagA+ or cagA- strains of H. pylori compared with uninfected. Although a trend of increased mucus thickness with cagA+ infection was observed.

KW - Gastric

KW - Helicobacter pylori

KW - Mucus

KW - Thickness

UR - http://www.scopus.com/inward/record.url?scp=27744514790&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=27744514790&partnerID=8YFLogxK

U2 - 10.1111/j.1440-1746.2005.03853.x

DO - 10.1111/j.1440-1746.2005.03853.x

M3 - Article

C2 - 16048574

AN - SCOPUS:27744514790

VL - 20

SP - 1246

EP - 1252

JO - Journal of Gastroenterology and Hepatology (Australia)

JF - Journal of Gastroenterology and Hepatology (Australia)

SN - 0815-9319

IS - 8

ER -