Effect of sitagliptin on the working memory and reference memory in type 2 diabetic sprague-dawley rats

Possible role of adiponectin receptors 1

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Type 2 diabetes mellitus (DM) is associated with increased incidence of behavioral changes and memory loss. Memory loss could be caused by Alzheimer's disease (AD) and vascular dementia (VaD). So, we aimed to investigate the effect of sitagliptin in improving the working and reference memories in diabetic rats. Thirty six male Sprague-Dawley rats divided equally (n=12) into three groups: control, type 2 DM and type 2 DM treated with DPP-4 inhibitor (sitagliptin) for one month (10 mg/kg) orally. Working memory and reference memory were assessed by using the hole board memory test. In all rats, serum glucose, insulin, adiponectin, total cholesterol (TC), TG, low (LDL) and high (HDL) density lipoprotein with calculation of the homeostasis model of assessment-insulin resistance index (HOMA-IR) and atherogenic index. The hypothalamus was separated for determination of the acetylcholine level and adiponectin receptors 1 (Adipo R1) m-RNA expression. Type 2 diabetic rats exhibited a significant decrease in both working and reference memories, with increased glucose, insulin and HOMA-IR. The adiponectin level, acetylcholine content of the hypothalamus and Adipo R1 m-RNA expression were significantly reduced. Treatment with sitagliptin significantly improved the working and reference memories with significant reduction in the glucose, insulin and HOMA-IR. Moreover, sitagliptin increased significantly the acetylcholine content of the hypothalamus and Adipo R1 expression. In conclusion, sitagliptin might improve the cognitive function of the diabetic rats and the hypothalamic acetylcholine level possibly through increased AdipoR1 expression.

Original languageEnglish
Pages (from-to)613-623
Number of pages11
JournalJournal of Physiology and Pharmacology
Volume64
Issue number5
Publication statusPublished - Oct 2013

Fingerprint

Adiponectin Receptors
Short-Term Memory
Sprague Dawley Rats
Type 2 Diabetes Mellitus
Hypothalamus
Acetylcholine
Insulin Resistance
Homeostasis
Adiponectin
Memory Disorders
HDL Lipoproteins
Insulin
Glucose
Alzheimer Disease
RNA
Vascular Dementia
Cholinergic Receptors
Cognition
Cholesterol
Sitagliptin Phosphate

Keywords

  • Acetylcholine
  • Adiponectin
  • Adiponectin receptors 1
  • Dementia
  • Diabetes mellitus type 2
  • Glucose
  • Insulin
  • Sitagliptin
  • Working memory

ASJC Scopus subject areas

  • Physiology
  • Pharmacology

Cite this

@article{4a096193cecf490280ba073122fca05a,
title = "Effect of sitagliptin on the working memory and reference memory in type 2 diabetic sprague-dawley rats: Possible role of adiponectin receptors 1",
abstract = "Type 2 diabetes mellitus (DM) is associated with increased incidence of behavioral changes and memory loss. Memory loss could be caused by Alzheimer's disease (AD) and vascular dementia (VaD). So, we aimed to investigate the effect of sitagliptin in improving the working and reference memories in diabetic rats. Thirty six male Sprague-Dawley rats divided equally (n=12) into three groups: control, type 2 DM and type 2 DM treated with DPP-4 inhibitor (sitagliptin) for one month (10 mg/kg) orally. Working memory and reference memory were assessed by using the hole board memory test. In all rats, serum glucose, insulin, adiponectin, total cholesterol (TC), TG, low (LDL) and high (HDL) density lipoprotein with calculation of the homeostasis model of assessment-insulin resistance index (HOMA-IR) and atherogenic index. The hypothalamus was separated for determination of the acetylcholine level and adiponectin receptors 1 (Adipo R1) m-RNA expression. Type 2 diabetic rats exhibited a significant decrease in both working and reference memories, with increased glucose, insulin and HOMA-IR. The adiponectin level, acetylcholine content of the hypothalamus and Adipo R1 m-RNA expression were significantly reduced. Treatment with sitagliptin significantly improved the working and reference memories with significant reduction in the glucose, insulin and HOMA-IR. Moreover, sitagliptin increased significantly the acetylcholine content of the hypothalamus and Adipo R1 expression. In conclusion, sitagliptin might improve the cognitive function of the diabetic rats and the hypothalamic acetylcholine level possibly through increased AdipoR1 expression.",
keywords = "Acetylcholine, Adiponectin, Adiponectin receptors 1, Dementia, Diabetes mellitus type 2, Glucose, Insulin, Sitagliptin, Working memory",
author = "Sakr, {H. F.}",
year = "2013",
month = "10",
language = "English",
volume = "64",
pages = "613--623",
journal = "Journal of Physiology and Pharmacology",
issn = "0867-5910",
publisher = "Polish Physiological Society",
number = "5",

}

TY - JOUR

T1 - Effect of sitagliptin on the working memory and reference memory in type 2 diabetic sprague-dawley rats

T2 - Possible role of adiponectin receptors 1

AU - Sakr, H. F.

PY - 2013/10

Y1 - 2013/10

N2 - Type 2 diabetes mellitus (DM) is associated with increased incidence of behavioral changes and memory loss. Memory loss could be caused by Alzheimer's disease (AD) and vascular dementia (VaD). So, we aimed to investigate the effect of sitagliptin in improving the working and reference memories in diabetic rats. Thirty six male Sprague-Dawley rats divided equally (n=12) into three groups: control, type 2 DM and type 2 DM treated with DPP-4 inhibitor (sitagliptin) for one month (10 mg/kg) orally. Working memory and reference memory were assessed by using the hole board memory test. In all rats, serum glucose, insulin, adiponectin, total cholesterol (TC), TG, low (LDL) and high (HDL) density lipoprotein with calculation of the homeostasis model of assessment-insulin resistance index (HOMA-IR) and atherogenic index. The hypothalamus was separated for determination of the acetylcholine level and adiponectin receptors 1 (Adipo R1) m-RNA expression. Type 2 diabetic rats exhibited a significant decrease in both working and reference memories, with increased glucose, insulin and HOMA-IR. The adiponectin level, acetylcholine content of the hypothalamus and Adipo R1 m-RNA expression were significantly reduced. Treatment with sitagliptin significantly improved the working and reference memories with significant reduction in the glucose, insulin and HOMA-IR. Moreover, sitagliptin increased significantly the acetylcholine content of the hypothalamus and Adipo R1 expression. In conclusion, sitagliptin might improve the cognitive function of the diabetic rats and the hypothalamic acetylcholine level possibly through increased AdipoR1 expression.

AB - Type 2 diabetes mellitus (DM) is associated with increased incidence of behavioral changes and memory loss. Memory loss could be caused by Alzheimer's disease (AD) and vascular dementia (VaD). So, we aimed to investigate the effect of sitagliptin in improving the working and reference memories in diabetic rats. Thirty six male Sprague-Dawley rats divided equally (n=12) into three groups: control, type 2 DM and type 2 DM treated with DPP-4 inhibitor (sitagliptin) for one month (10 mg/kg) orally. Working memory and reference memory were assessed by using the hole board memory test. In all rats, serum glucose, insulin, adiponectin, total cholesterol (TC), TG, low (LDL) and high (HDL) density lipoprotein with calculation of the homeostasis model of assessment-insulin resistance index (HOMA-IR) and atherogenic index. The hypothalamus was separated for determination of the acetylcholine level and adiponectin receptors 1 (Adipo R1) m-RNA expression. Type 2 diabetic rats exhibited a significant decrease in both working and reference memories, with increased glucose, insulin and HOMA-IR. The adiponectin level, acetylcholine content of the hypothalamus and Adipo R1 m-RNA expression were significantly reduced. Treatment with sitagliptin significantly improved the working and reference memories with significant reduction in the glucose, insulin and HOMA-IR. Moreover, sitagliptin increased significantly the acetylcholine content of the hypothalamus and Adipo R1 expression. In conclusion, sitagliptin might improve the cognitive function of the diabetic rats and the hypothalamic acetylcholine level possibly through increased AdipoR1 expression.

KW - Acetylcholine

KW - Adiponectin

KW - Adiponectin receptors 1

KW - Dementia

KW - Diabetes mellitus type 2

KW - Glucose

KW - Insulin

KW - Sitagliptin

KW - Working memory

UR - http://www.scopus.com/inward/record.url?scp=84888235042&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84888235042&partnerID=8YFLogxK

M3 - Article

VL - 64

SP - 613

EP - 623

JO - Journal of Physiology and Pharmacology

JF - Journal of Physiology and Pharmacology

SN - 0867-5910

IS - 5

ER -