Effect of selenium and grape seed extract on indomethacin-induced gastric ulcers in rats

Amr M. Abbas, Hussein F. Sakr

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Indomethacin (IND) is a non-steroid antiinflammatory agent that is known to induce severe gastric mucosal lesions. In this study, we investigated the protective effect of selenium (SEL), grape seed extract (GSE), and both on IND-induced gastric mucosal ulcers in rats. Sprague-Dawley rats (200-250 g) were given SEL, GSE, and both by oral gavage for 28 days, and then gastric ulcers were induced by oral administration of 25 mg/kg IND. Malondialdehyde (MDA), non-enzymatic (reduced glutathione, GSH) and enzymatic (superoxide dismutase, catalase, and glutathione peroxidase) antioxidants, prostaglandin E2 (PGE2) in gastric mucosa, and serum tumor necrosis factor alpha (TNF-α) were measured. Moreover, gastric ulcer index and preventive index were determined. Indomethacin increased the gastric ulcer index, MDA, TNF-α, and decreased PGE2 and non-enzymatic (GSH) and enzymatic (superoxide dismutase, catalase, and glutathione peroxidase) antioxidants. Pretreatment with SEL, GSE, and both significantly decreased the gastric ulcer index, MDA, and TNF and increased antioxidants and PGE2. Histopathological observations confirm the gastric ulcer index and biochemical parameters. Selenium and GSE have a protective effect against IND-induced gastric ulcers through prevention of lipid peroxidation, increase of GSH, activation of radical scavenging enzymes, PGE2 generation, and antiinflammatory activity. Co-administration of GSE and SEL is more effective than GSE or SEL alone.

Original languageEnglish
Pages (from-to)527-537
Number of pages11
JournalJournal of Physiology and Biochemistry
Volume69
Issue number3
DOIs
Publication statusPublished - 2013

Fingerprint

Grape Seed Extract
Stomach Ulcer
Selenium
Indomethacin
Rats
Dinoprostone
Malondialdehyde
Antioxidants
Glutathione Peroxidase
Catalase
Superoxide Dismutase
Anti-Inflammatory Agents
Tumor Necrosis Factor-alpha
Scavenging
Glutathione
Gastric Mucosa
Lipid Peroxidation
Oral Administration
Sprague Dawley Rats
Chemical activation

Keywords

  • Gastric ulcer
  • Grape seed extract
  • Indomethacin
  • Selenium

ASJC Scopus subject areas

  • Physiology
  • Biochemistry

Cite this

Effect of selenium and grape seed extract on indomethacin-induced gastric ulcers in rats. / Abbas, Amr M.; Sakr, Hussein F.

In: Journal of Physiology and Biochemistry, Vol. 69, No. 3, 2013, p. 527-537.

Research output: Contribution to journalArticle

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AB - Indomethacin (IND) is a non-steroid antiinflammatory agent that is known to induce severe gastric mucosal lesions. In this study, we investigated the protective effect of selenium (SEL), grape seed extract (GSE), and both on IND-induced gastric mucosal ulcers in rats. Sprague-Dawley rats (200-250 g) were given SEL, GSE, and both by oral gavage for 28 days, and then gastric ulcers were induced by oral administration of 25 mg/kg IND. Malondialdehyde (MDA), non-enzymatic (reduced glutathione, GSH) and enzymatic (superoxide dismutase, catalase, and glutathione peroxidase) antioxidants, prostaglandin E2 (PGE2) in gastric mucosa, and serum tumor necrosis factor alpha (TNF-α) were measured. Moreover, gastric ulcer index and preventive index were determined. Indomethacin increased the gastric ulcer index, MDA, TNF-α, and decreased PGE2 and non-enzymatic (GSH) and enzymatic (superoxide dismutase, catalase, and glutathione peroxidase) antioxidants. Pretreatment with SEL, GSE, and both significantly decreased the gastric ulcer index, MDA, and TNF and increased antioxidants and PGE2. Histopathological observations confirm the gastric ulcer index and biochemical parameters. Selenium and GSE have a protective effect against IND-induced gastric ulcers through prevention of lipid peroxidation, increase of GSH, activation of radical scavenging enzymes, PGE2 generation, and antiinflammatory activity. Co-administration of GSE and SEL is more effective than GSE or SEL alone.

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