Effect of salinity on pythium damping-off of cucumber and on the tolerance of Pythium aphanidermatum

A. M. Al-Sadi, R. S. Al-Masoudi, N. Al-Habsi, F. A. Al-Said, S. A. Al-Rawahy, M. Ahmed, M. L. Deadman

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

A study was conducted to investigate the effect of salinity on pythium damping-off and tolerance to salinity among Pythium aphanidermatum isolates from different geographical origins, genetic backgrounds and saline/non-saline soils. Increasing irrigation-water salinity from 0·01 to 5 dS m -1 significantly increased mortality in cucumber seedlings inoculated with P. aphanidermatum and reduced dry weight of non-inoculated seedlings. In vitro tests in culture media amended with different concentrations of NaCl showed that growth of P. aphanidermatum, P. spinosum and P. splendens isolates was stimulated or unaffected at salinity levels stressful for cucumber (electrical conductivity = 5 dS m -1). Significant differences were found in tolerance to salinity among 47 P. aphanidermatum isolates obtained from different greenhouses. The concentration of NaCl required to reduce growth of P. aphanidermatum isolates by 50% varied from 23 to 62 dS m -1, with an average of 46 dS m -1, however, oospore production was more sensitive to salinity and no oospores were produced above 20 dS m -1. Analysis of tolerance of P. aphanidermatum isolates to salinity showed no relationship between tolerance to salinity and geographical origins or genetic data. Isolates of P. aphanidermatum from greenhouses with no salinity problems were as tolerant to salinity as isolates obtained from salinity-affected greenhouses, suggesting lack of evidence for ecological adaptation within P. aphanidermatum in Oman. Increased mortality in cucumber seedlings at higher salinity levels may imply a synergistic interaction between salinity stress and salinity-tolerant Pythium species on cucumber seedlings, resulting in greater seedling losses.

Original languageEnglish
Pages (from-to)112-120
Number of pages9
JournalPlant Pathology
Volume59
Issue number1
DOIs
Publication statusPublished - Feb 2010

Fingerprint

Pythium
Pythium aphanidermatum
Cucumis sativus
damping off
Salinity
cucumbers
salinity
Seedlings
seedlings
oospores
greenhouses
Oman
Electric Conductivity
water salinity
Mortality
genetic background
irrigation water
electrical conductivity
salt stress
Growth

Keywords

  • AFLP
  • Cucumis sativus
  • Pythium aphanidermatum
  • Pythium oligandrum
  • Pythium spinosum
  • Pythium splendens

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science
  • Horticulture
  • Genetics

Cite this

Effect of salinity on pythium damping-off of cucumber and on the tolerance of Pythium aphanidermatum. / Al-Sadi, A. M.; Al-Masoudi, R. S.; Al-Habsi, N.; Al-Said, F. A.; Al-Rawahy, S. A.; Ahmed, M.; Deadman, M. L.

In: Plant Pathology, Vol. 59, No. 1, 02.2010, p. 112-120.

Research output: Contribution to journalArticle

Al-Sadi, A. M. ; Al-Masoudi, R. S. ; Al-Habsi, N. ; Al-Said, F. A. ; Al-Rawahy, S. A. ; Ahmed, M. ; Deadman, M. L. / Effect of salinity on pythium damping-off of cucumber and on the tolerance of Pythium aphanidermatum. In: Plant Pathology. 2010 ; Vol. 59, No. 1. pp. 112-120.
@article{a3359fe9b4b84347a19126a20382a1b7,
title = "Effect of salinity on pythium damping-off of cucumber and on the tolerance of Pythium aphanidermatum",
abstract = "A study was conducted to investigate the effect of salinity on pythium damping-off and tolerance to salinity among Pythium aphanidermatum isolates from different geographical origins, genetic backgrounds and saline/non-saline soils. Increasing irrigation-water salinity from 0·01 to 5 dS m -1 significantly increased mortality in cucumber seedlings inoculated with P. aphanidermatum and reduced dry weight of non-inoculated seedlings. In vitro tests in culture media amended with different concentrations of NaCl showed that growth of P. aphanidermatum, P. spinosum and P. splendens isolates was stimulated or unaffected at salinity levels stressful for cucumber (electrical conductivity = 5 dS m -1). Significant differences were found in tolerance to salinity among 47 P. aphanidermatum isolates obtained from different greenhouses. The concentration of NaCl required to reduce growth of P. aphanidermatum isolates by 50{\%} varied from 23 to 62 dS m -1, with an average of 46 dS m -1, however, oospore production was more sensitive to salinity and no oospores were produced above 20 dS m -1. Analysis of tolerance of P. aphanidermatum isolates to salinity showed no relationship between tolerance to salinity and geographical origins or genetic data. Isolates of P. aphanidermatum from greenhouses with no salinity problems were as tolerant to salinity as isolates obtained from salinity-affected greenhouses, suggesting lack of evidence for ecological adaptation within P. aphanidermatum in Oman. Increased mortality in cucumber seedlings at higher salinity levels may imply a synergistic interaction between salinity stress and salinity-tolerant Pythium species on cucumber seedlings, resulting in greater seedling losses.",
keywords = "AFLP, Cucumis sativus, Pythium aphanidermatum, Pythium oligandrum, Pythium spinosum, Pythium splendens",
author = "Al-Sadi, {A. M.} and Al-Masoudi, {R. S.} and N. Al-Habsi and Al-Said, {F. A.} and Al-Rawahy, {S. A.} and M. Ahmed and Deadman, {M. L.}",
year = "2010",
month = "2",
doi = "10.1111/j.1365-3059.2009.02176.x",
language = "English",
volume = "59",
pages = "112--120",
journal = "Plant Pathology",
issn = "0032-0862",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Effect of salinity on pythium damping-off of cucumber and on the tolerance of Pythium aphanidermatum

AU - Al-Sadi, A. M.

AU - Al-Masoudi, R. S.

AU - Al-Habsi, N.

AU - Al-Said, F. A.

AU - Al-Rawahy, S. A.

AU - Ahmed, M.

AU - Deadman, M. L.

PY - 2010/2

Y1 - 2010/2

N2 - A study was conducted to investigate the effect of salinity on pythium damping-off and tolerance to salinity among Pythium aphanidermatum isolates from different geographical origins, genetic backgrounds and saline/non-saline soils. Increasing irrigation-water salinity from 0·01 to 5 dS m -1 significantly increased mortality in cucumber seedlings inoculated with P. aphanidermatum and reduced dry weight of non-inoculated seedlings. In vitro tests in culture media amended with different concentrations of NaCl showed that growth of P. aphanidermatum, P. spinosum and P. splendens isolates was stimulated or unaffected at salinity levels stressful for cucumber (electrical conductivity = 5 dS m -1). Significant differences were found in tolerance to salinity among 47 P. aphanidermatum isolates obtained from different greenhouses. The concentration of NaCl required to reduce growth of P. aphanidermatum isolates by 50% varied from 23 to 62 dS m -1, with an average of 46 dS m -1, however, oospore production was more sensitive to salinity and no oospores were produced above 20 dS m -1. Analysis of tolerance of P. aphanidermatum isolates to salinity showed no relationship between tolerance to salinity and geographical origins or genetic data. Isolates of P. aphanidermatum from greenhouses with no salinity problems were as tolerant to salinity as isolates obtained from salinity-affected greenhouses, suggesting lack of evidence for ecological adaptation within P. aphanidermatum in Oman. Increased mortality in cucumber seedlings at higher salinity levels may imply a synergistic interaction between salinity stress and salinity-tolerant Pythium species on cucumber seedlings, resulting in greater seedling losses.

AB - A study was conducted to investigate the effect of salinity on pythium damping-off and tolerance to salinity among Pythium aphanidermatum isolates from different geographical origins, genetic backgrounds and saline/non-saline soils. Increasing irrigation-water salinity from 0·01 to 5 dS m -1 significantly increased mortality in cucumber seedlings inoculated with P. aphanidermatum and reduced dry weight of non-inoculated seedlings. In vitro tests in culture media amended with different concentrations of NaCl showed that growth of P. aphanidermatum, P. spinosum and P. splendens isolates was stimulated or unaffected at salinity levels stressful for cucumber (electrical conductivity = 5 dS m -1). Significant differences were found in tolerance to salinity among 47 P. aphanidermatum isolates obtained from different greenhouses. The concentration of NaCl required to reduce growth of P. aphanidermatum isolates by 50% varied from 23 to 62 dS m -1, with an average of 46 dS m -1, however, oospore production was more sensitive to salinity and no oospores were produced above 20 dS m -1. Analysis of tolerance of P. aphanidermatum isolates to salinity showed no relationship between tolerance to salinity and geographical origins or genetic data. Isolates of P. aphanidermatum from greenhouses with no salinity problems were as tolerant to salinity as isolates obtained from salinity-affected greenhouses, suggesting lack of evidence for ecological adaptation within P. aphanidermatum in Oman. Increased mortality in cucumber seedlings at higher salinity levels may imply a synergistic interaction between salinity stress and salinity-tolerant Pythium species on cucumber seedlings, resulting in greater seedling losses.

KW - AFLP

KW - Cucumis sativus

KW - Pythium aphanidermatum

KW - Pythium oligandrum

KW - Pythium spinosum

KW - Pythium splendens

UR - http://www.scopus.com/inward/record.url?scp=74249108812&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=74249108812&partnerID=8YFLogxK

U2 - 10.1111/j.1365-3059.2009.02176.x

DO - 10.1111/j.1365-3059.2009.02176.x

M3 - Article

VL - 59

SP - 112

EP - 120

JO - Plant Pathology

JF - Plant Pathology

SN - 0032-0862

IS - 1

ER -