Effect of nalidixic acid on the morphology and protein expression of Pseudomonas aeruginosa

Saif Al Bahry, Nallusamy Sivakumar, Manal Al-Khambashi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objective: To determine the effect of nalidixic acid on the morphology and protein expression of Pseudomonas aeruginosa (P. aeruginosa). Methods: Nalidixic acid solution of 1 600 μg/mL was prepared. The minimum inhibitory concentration (MIC) for P. aeruginosa was determined with tube dilution test. The effect of nalidixic acid on the morphology of P. aeruginosa was studied using light microscope and scanning electron microscope. Changes in protein profile were studied using SDS-PAGE. Results: The MIC of nalidixic acid was 700 μg/mL against P. aeruginosa. The exposure of P. aeruginosa to different concentrations of nalidixic acid resulted in deformation of most of the growing cells. At the concentration of 600 μg/mL most of the cells turned into elongated and adhere to each other while some of the cells were bulged. The intensity of protein bands were changed when they exposed to nalidixic acid. Conclusions: The present findings suggest that the morphology and protein expression of P. aeruginosa is greatly affected by nalidixic acid.

Original languageEnglish
Pages (from-to)265-269
Number of pages5
JournalAsian Pacific Journal of Tropical Medicine
Volume5
Issue number4
DOIs
Publication statusPublished - Apr 2012

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Nalidixic Acid
Pseudomonas aeruginosa
Proteins
Microbial Sensitivity Tests
Polyacrylamide Gel Electrophoresis
Electrons
Light

Keywords

  • Deformed cells
  • Nalidixic acid
  • Pseudomonas aeruginosa
  • Scanning electron microscopy

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Effect of nalidixic acid on the morphology and protein expression of Pseudomonas aeruginosa. / Al Bahry, Saif; Sivakumar, Nallusamy; Al-Khambashi, Manal.

In: Asian Pacific Journal of Tropical Medicine, Vol. 5, No. 4, 04.2012, p. 265-269.

Research output: Contribution to journalArticle

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