Effect of mucin and glucose on proteolytic and glycosidic activities of Streptococcus oralis

A. M. Rafay, K. A. Homer, D. Beighton

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The production of glycosidase and protease activities, which may play a role in the degradation of human glycoproteins, by Streptococcus oralis strains isolated from endocarditis, septicaemia or the oral cavity was investigated with a range of fluorogenic substrates. The pH optima of the proteases ranged from 6.0 to 9.3 and the pH optima for the glycosidases were lower (4.5-6.0), although the pH range over which both groups of enzymes acted was broad. Growth in a minimal medium supplemented with glucose resulted in repression of glycosidase activities and elevated proteolytic activity. Bacteria from cultures supplemented with porcine gastric mucin (PGM), a model glycoprotein, exhibited higher levels of glycosidase activity, while proteolytic activity was suppressed and glycoprotein-derived monosaccharides were transported at significantly higher rates than those observed for cells grown in media with glucose. PGM-derived cells also exhibited high levels of N-acetylneuraminate pyruvate-lyase, the first intracellular enzyme in the pathway of sialic acid catabolism. Taken together, these data indicate that S. oralis strains produce a range of proteolytic and glycosidic enzymes that may play a role in the degradation of host-derived glycoproteins.

Original languageEnglish
Pages (from-to)409-417
Number of pages9
JournalJournal of Medical Microbiology
Volume44
Issue number6
Publication statusPublished - 1996

Fingerprint

Streptococcus oralis
Glycoside Hydrolases
Mucins
Glycoproteins
Gastric Mucins
Glucose
Peptide Hydrolases
N-acetylneuraminate lyase
Swine
Monosaccharides
N-Acetylneuraminic Acid
Enzymes
Endocarditis
Fluorescent Dyes
Mouth
Sepsis
Bacteria
Growth

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Effect of mucin and glucose on proteolytic and glycosidic activities of Streptococcus oralis. / Rafay, A. M.; Homer, K. A.; Beighton, D.

In: Journal of Medical Microbiology, Vol. 44, No. 6, 1996, p. 409-417.

Research output: Contribution to journalArticle

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