Effect of juvenile hormone and serotonin (5-HT) on mixis induction of the rotifer Brachionus plicatilis Muller

Wenresti G. Gallardo, Atsushi Hagiwara, Terry W. Snell

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Juvenile hormone (JH) and serotonin (5-HT) were previously shown to enhance mictic (sexual) female production of the rotifer Brachionus plicatilis in batch cultures. To explore the basis of these effects, experiments were conducted on isolated individuals. JH treatment of maternal rotifers with 5 and 50 μg ml-1 (18.8 and 187.7 μM) resulted in significantly higher (P2) and third (F3) generations. JH treatment was effective even at a lower food concentration of 7 x 105 cells ml-1, but it was not effective when free ammonia was added at 2.4 and 3.1 μg ml-1. Mictic female production was not increased with exposure to 5-HT up to 50 μg ml-1 (129.1 μM) concentrations. When food level was reduced to 7 x 105 cells ml-1, however, 5-HT-treated rotifers produced significantly (P <0.05) more mictic females than the control, particularly in F3 generation. Mictic female production of 5-HT-treated rotifers did not differ from that of the control with or without free ammonia, but the intrinsic rate of natural increase (r) of 5-HT-treated rotifers at 3.1 μg ml-1 free ammonia was significantly higher than the control. These results show that juvenile hormone increases mictic female production under optimum and sub-optimum food levels, whereas 5-HT increases both mictic female production at low food level and population growth rate at high free ammonia concentrations. These compounds could be used to manage rotifer cultures and probe the mechanisms controlling the rotifer life cycle as it switches to mictic reproduction. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)97-107
Number of pages11
JournalJournal of Experimental Marine Biology and Ecology
Volume252
Issue number1
DOIs
Publication statusPublished - 2000

Keywords

  • 5-Hydroxytryptamine
  • Brachionus plicatilis
  • Food level
  • Free ammonia
  • Juvenile hormone
  • Mictic female
  • Rotifera
  • Sexual and asexual reproduction

ASJC Scopus subject areas

  • Aquatic Science
  • Ecology, Evolution, Behavior and Systematics
  • Ecology

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