Abstract
Juvenile hormone (JH) and serotonin (5-HT) were previously shown to enhance mictic (sexual) female production of the rotifer Brachionus plicatilis in batch cultures. To explore the basis of these effects, experiments were conducted on isolated individuals. JH treatment of maternal rotifers with 5 and 50 μg ml-1 (18.8 and 187.7 μM) resulted in significantly higher (P2) and third (F3) generations. JH treatment was effective even at a lower food concentration of 7 x 105 cells ml-1, but it was not effective when free ammonia was added at 2.4 and 3.1 μg ml-1. Mictic female production was not increased with exposure to 5-HT up to 50 μg ml-1 (129.1 μM) concentrations. When food level was reduced to 7 x 105 cells ml-1, however, 5-HT-treated rotifers produced significantly (P <0.05) more mictic females than the control, particularly in F3 generation. Mictic female production of 5-HT-treated rotifers did not differ from that of the control with or without free ammonia, but the intrinsic rate of natural increase (r) of 5-HT-treated rotifers at 3.1 μg ml-1 free ammonia was significantly higher than the control. These results show that juvenile hormone increases mictic female production under optimum and sub-optimum food levels, whereas 5-HT increases both mictic female production at low food level and population growth rate at high free ammonia concentrations. These compounds could be used to manage rotifer cultures and probe the mechanisms controlling the rotifer life cycle as it switches to mictic reproduction. (C) 2000 Elsevier Science B.V.
| Original language | English |
|---|---|
| Pages (from-to) | 97-107 |
| Number of pages | 11 |
| Journal | Journal of Experimental Marine Biology and Ecology |
| Volume | 252 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2000 |
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Keywords
- 5-Hydroxytryptamine
- Brachionus plicatilis
- Food level
- Free ammonia
- Juvenile hormone
- Mictic female
- Rotifera
- Sexual and asexual reproduction
ASJC Scopus subject areas
- Aquatic Science
- Ecology, Evolution, Behavior and Systematics
- Ecology
Cite this
Effect of juvenile hormone and serotonin (5-HT) on mixis induction of the rotifer Brachionus plicatilis Muller. / Gallardo, Wenresti G.; Hagiwara, Atsushi; Snell, Terry W.
In: Journal of Experimental Marine Biology and Ecology, Vol. 252, No. 1, 2000, p. 97-107.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of juvenile hormone and serotonin (5-HT) on mixis induction of the rotifer Brachionus plicatilis Muller
AU - Gallardo, Wenresti G.
AU - Hagiwara, Atsushi
AU - Snell, Terry W.
PY - 2000
Y1 - 2000
N2 - Juvenile hormone (JH) and serotonin (5-HT) were previously shown to enhance mictic (sexual) female production of the rotifer Brachionus plicatilis in batch cultures. To explore the basis of these effects, experiments were conducted on isolated individuals. JH treatment of maternal rotifers with 5 and 50 μg ml-1 (18.8 and 187.7 μM) resulted in significantly higher (P2) and third (F3) generations. JH treatment was effective even at a lower food concentration of 7 x 105 cells ml-1, but it was not effective when free ammonia was added at 2.4 and 3.1 μg ml-1. Mictic female production was not increased with exposure to 5-HT up to 50 μg ml-1 (129.1 μM) concentrations. When food level was reduced to 7 x 105 cells ml-1, however, 5-HT-treated rotifers produced significantly (P <0.05) more mictic females than the control, particularly in F3 generation. Mictic female production of 5-HT-treated rotifers did not differ from that of the control with or without free ammonia, but the intrinsic rate of natural increase (r) of 5-HT-treated rotifers at 3.1 μg ml-1 free ammonia was significantly higher than the control. These results show that juvenile hormone increases mictic female production under optimum and sub-optimum food levels, whereas 5-HT increases both mictic female production at low food level and population growth rate at high free ammonia concentrations. These compounds could be used to manage rotifer cultures and probe the mechanisms controlling the rotifer life cycle as it switches to mictic reproduction. (C) 2000 Elsevier Science B.V.
AB - Juvenile hormone (JH) and serotonin (5-HT) were previously shown to enhance mictic (sexual) female production of the rotifer Brachionus plicatilis in batch cultures. To explore the basis of these effects, experiments were conducted on isolated individuals. JH treatment of maternal rotifers with 5 and 50 μg ml-1 (18.8 and 187.7 μM) resulted in significantly higher (P2) and third (F3) generations. JH treatment was effective even at a lower food concentration of 7 x 105 cells ml-1, but it was not effective when free ammonia was added at 2.4 and 3.1 μg ml-1. Mictic female production was not increased with exposure to 5-HT up to 50 μg ml-1 (129.1 μM) concentrations. When food level was reduced to 7 x 105 cells ml-1, however, 5-HT-treated rotifers produced significantly (P <0.05) more mictic females than the control, particularly in F3 generation. Mictic female production of 5-HT-treated rotifers did not differ from that of the control with or without free ammonia, but the intrinsic rate of natural increase (r) of 5-HT-treated rotifers at 3.1 μg ml-1 free ammonia was significantly higher than the control. These results show that juvenile hormone increases mictic female production under optimum and sub-optimum food levels, whereas 5-HT increases both mictic female production at low food level and population growth rate at high free ammonia concentrations. These compounds could be used to manage rotifer cultures and probe the mechanisms controlling the rotifer life cycle as it switches to mictic reproduction. (C) 2000 Elsevier Science B.V.
KW - 5-Hydroxytryptamine
KW - Brachionus plicatilis
KW - Food level
KW - Free ammonia
KW - Juvenile hormone
KW - Mictic female
KW - Rotifera
KW - Sexual and asexual reproduction
UR - http://www.scopus.com/inward/record.url?scp=0033835629&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033835629&partnerID=8YFLogxK
U2 - 10.1016/S0022-0981(00)00240-9
DO - 10.1016/S0022-0981(00)00240-9
M3 - Article
AN - SCOPUS:0033835629
VL - 252
SP - 97
EP - 107
JO - Journal of Experimental Marine Biology and Ecology
JF - Journal of Experimental Marine Biology and Ecology
SN - 0022-0981
IS - 1
ER -