Effect of infection with the ts22 mutant of Semliki Forest virus on development of the central nervous system in the fetal mouse

M. J.E.M.F. Mabruk, G. M. Glasgow, A. M. Flack, J. C. Folan, J. G. Bannigan, J. M.B. Smyth, M. A. O'Sullivan, B. J. Sheahan, G. J. Atkins

Research output: Contribution to journalArticle

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Abstract

The A7 strain of Semliki Forest virus induces rapid fetal death in pregnant mice, whereas the ts22 mutant derived from it is teratogenic for a proportion of fetuses. Both A7 and ts22 induce viremia and infect the central nervous systems and fetuses of pregnant mice. Using immunogold-silver staining, a cDNA probe for a Semliki Forest virus nonstructural sequence, and a riboprobe derived from the same sequence, we showed that the skin and musculoskeletal systems of fetuses from mothers infected with ts22 were often heavily infected but the central nervous systems were not labeled before day 17 of pregnancy. Damage to the neural tube, including open-neural-tube defects, was detected in fetuses following infection of the mother at days 8 and 10 of pregnancy with both A7 and ts22. For ts22, neural tube damage induced by fetal infection before day 17 of pregnancy appeared to be indirect and caused by virus infection of mesenchymal cells surrounding the developing neural tube.

Original languageEnglish
Pages (from-to)4027-4033
Number of pages7
JournalJournal of Virology
Volume63
Issue number9
Publication statusPublished - 1989

Fingerprint

Semliki Forest virus
Semliki forest virus
neurodevelopment
Neural Tube
fetus
Fetus
Central Nervous System
mutants
mice
pregnancy
Infection
infection
Pregnancy
central nervous system
neural tube defects
musculoskeletal system
Musculoskeletal System
Silver Staining
Fetal Death
Neural Tube Defects

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Mabruk, M. J. E. M. F., Glasgow, G. M., Flack, A. M., Folan, J. C., Bannigan, J. G., Smyth, J. M. B., ... Atkins, G. J. (1989). Effect of infection with the ts22 mutant of Semliki Forest virus on development of the central nervous system in the fetal mouse. Journal of Virology, 63(9), 4027-4033.

Effect of infection with the ts22 mutant of Semliki Forest virus on development of the central nervous system in the fetal mouse. / Mabruk, M. J.E.M.F.; Glasgow, G. M.; Flack, A. M.; Folan, J. C.; Bannigan, J. G.; Smyth, J. M.B.; O'Sullivan, M. A.; Sheahan, B. J.; Atkins, G. J.

In: Journal of Virology, Vol. 63, No. 9, 1989, p. 4027-4033.

Research output: Contribution to journalArticle

Mabruk, MJEMF, Glasgow, GM, Flack, AM, Folan, JC, Bannigan, JG, Smyth, JMB, O'Sullivan, MA, Sheahan, BJ & Atkins, GJ 1989, 'Effect of infection with the ts22 mutant of Semliki Forest virus on development of the central nervous system in the fetal mouse', Journal of Virology, vol. 63, no. 9, pp. 4027-4033.
Mabruk, M. J.E.M.F. ; Glasgow, G. M. ; Flack, A. M. ; Folan, J. C. ; Bannigan, J. G. ; Smyth, J. M.B. ; O'Sullivan, M. A. ; Sheahan, B. J. ; Atkins, G. J. / Effect of infection with the ts22 mutant of Semliki Forest virus on development of the central nervous system in the fetal mouse. In: Journal of Virology. 1989 ; Vol. 63, No. 9. pp. 4027-4033.
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