In vitro propagation of Vitis offers opportunities for increasing plant material for cultivation. Cultures were established and maintained in vitro on MS medium supplemented with BA (0, 5 and 10 μM) for shoot and NAA (0, 1,5 and 10 μM) for callus induction. The best explant sterilization was achieved with 10% chlorox treatment either for 10 or 15 minutes. Oxidative browning was effectively controlled by three subcultures of explants on fresh media. Better shooting was observed on MS medium with 5 μM BA. Shoot proliferation (80%) was obtained by subculturing the micro cuttings on same media as used for shoot formation. Maximum rooting (80%) occurred on medium with 10 μM IBA. The highest callus induction was 80% from stem segments on 5 μM BA followed by leaf disk explants on NAA (1 μM). All callus cultures initiated aerial roots. Leaf disk explants (60%) developed embryoids on MS medium supplemented with NAA (2 mg/1).
|Number of pages||5|
|Journal||Pakistan Journal of Botany|
|Publication status||Published - Feb 2008|
ASJC Scopus subject areas
- Plant Science