Effect of growth hormones on micropropagation of Vitis vinifera L. CV. perlette

Muhammad J. Jaskani, Haider Abbas, R. Sultana, M. M. Khan, M. Qasim, Iqrar A. Khan

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28 Citations (Scopus)

Abstract

In vitro propagation of Vitis offers opportunities for increasing plant material for cultivation. Cultures were established and maintained in vitro on MS medium supplemented with BA (0, 5 and 10 μM) for shoot and NAA (0, 1,5 and 10 μM) for callus induction. The best explant sterilization was achieved with 10% chlorox treatment either for 10 or 15 minutes. Oxidative browning was effectively controlled by three subcultures of explants on fresh media. Better shooting was observed on MS medium with 5 μM BA. Shoot proliferation (80%) was obtained by subculturing the micro cuttings on same media as used for shoot formation. Maximum rooting (80%) occurred on medium with 10 μM IBA. The highest callus induction was 80% from stem segments on 5 μM BA followed by leaf disk explants on NAA (1 μM). All callus cultures initiated aerial roots. Leaf disk explants (60%) developed embryoids on MS medium supplemented with NAA (2 mg/1).

Original languageEnglish
Pages (from-to)105-109
Number of pages5
JournalPakistan Journal of Botany
Volume40
Issue number1
Publication statusPublished - Feb 2008

ASJC Scopus subject areas

  • Plant Science

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    Jaskani, M. J., Abbas, H., Sultana, R., Khan, M. M., Qasim, M., & Khan, I. A. (2008). Effect of growth hormones on micropropagation of Vitis vinifera L. CV. perlette. Pakistan Journal of Botany, 40(1), 105-109.