Abstract
Growth hormone (GH, 0.0025 and 0.025 I.U. ml-1) and γ-aminobutyric acid (GABA, 50 μg ml-1) enhance rotifer population growth in batch cultures. In order to further understand the mechanism of their actions, we conducted experiments culturing isolated females at low food and high free ammonia levels. At an optimum food level of 7 x 106 Nannochloropsis oculata cells ml-1 or at low free ammonia level of 2.4 μg ml-1, the F1 offspring of rotifers treated with GH at 0.0025 I.U. ml-1 had significantly higher population growth rate (r) and net reproduction rate (Ro), and shorter generation time than untreated rotifers. At a lower food level of 7 x 105 cells ml-1 or at high free ammonia level of 3.1 μg ml-1, rotifers treated with GABA at 50 μg ml-1 had significantly higher r and Ro, and shorter generation time. These results indicate that GABA is effective in enhancing rotifer reproduction when rotifers are cultured under stress whereas GH enhances rotifer reproduction when culture conditions are optimal. Significant effects were also observed in F1 and F2 generations which were not treated with hormones. These data may be useful for treating rotifer mass cultures to mitigate the effects of stress caused by high population densities.
| Original language | English |
|---|---|
| Pages (from-to) | 179-191 |
| Number of pages | 13 |
| Journal | Journal of Experimental Marine Biology and Ecology |
| Volume | 240 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jul 15 1999 |
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Keywords
- γ-Aminobutyric acid
- Brachionus plicatilis
- Food limitation
- Free ammonia
- Growth hormone
- Reproduction
- Rotifera
ASJC Scopus subject areas
- Aquatic Science
- Ecology, Evolution, Behavior and Systematics
- Ecology
Cite this
Effect of growth hormone and γ-aminobutyric acid on Brachionus plicatilis (Rotifera) reproduction at low food or high ammonia levels. / Gallardo, Wenresti G.; Hagiwara, Atsushi; Tomita, Yuichi; Snell, Terry W.
In: Journal of Experimental Marine Biology and Ecology, Vol. 240, No. 2, 15.07.1999, p. 179-191.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of growth hormone and γ-aminobutyric acid on Brachionus plicatilis (Rotifera) reproduction at low food or high ammonia levels
AU - Gallardo, Wenresti G.
AU - Hagiwara, Atsushi
AU - Tomita, Yuichi
AU - Snell, Terry W.
PY - 1999/7/15
Y1 - 1999/7/15
N2 - Growth hormone (GH, 0.0025 and 0.025 I.U. ml-1) and γ-aminobutyric acid (GABA, 50 μg ml-1) enhance rotifer population growth in batch cultures. In order to further understand the mechanism of their actions, we conducted experiments culturing isolated females at low food and high free ammonia levels. At an optimum food level of 7 x 106 Nannochloropsis oculata cells ml-1 or at low free ammonia level of 2.4 μg ml-1, the F1 offspring of rotifers treated with GH at 0.0025 I.U. ml-1 had significantly higher population growth rate (r) and net reproduction rate (Ro), and shorter generation time than untreated rotifers. At a lower food level of 7 x 105 cells ml-1 or at high free ammonia level of 3.1 μg ml-1, rotifers treated with GABA at 50 μg ml-1 had significantly higher r and Ro, and shorter generation time. These results indicate that GABA is effective in enhancing rotifer reproduction when rotifers are cultured under stress whereas GH enhances rotifer reproduction when culture conditions are optimal. Significant effects were also observed in F1 and F2 generations which were not treated with hormones. These data may be useful for treating rotifer mass cultures to mitigate the effects of stress caused by high population densities.
AB - Growth hormone (GH, 0.0025 and 0.025 I.U. ml-1) and γ-aminobutyric acid (GABA, 50 μg ml-1) enhance rotifer population growth in batch cultures. In order to further understand the mechanism of their actions, we conducted experiments culturing isolated females at low food and high free ammonia levels. At an optimum food level of 7 x 106 Nannochloropsis oculata cells ml-1 or at low free ammonia level of 2.4 μg ml-1, the F1 offspring of rotifers treated with GH at 0.0025 I.U. ml-1 had significantly higher population growth rate (r) and net reproduction rate (Ro), and shorter generation time than untreated rotifers. At a lower food level of 7 x 105 cells ml-1 or at high free ammonia level of 3.1 μg ml-1, rotifers treated with GABA at 50 μg ml-1 had significantly higher r and Ro, and shorter generation time. These results indicate that GABA is effective in enhancing rotifer reproduction when rotifers are cultured under stress whereas GH enhances rotifer reproduction when culture conditions are optimal. Significant effects were also observed in F1 and F2 generations which were not treated with hormones. These data may be useful for treating rotifer mass cultures to mitigate the effects of stress caused by high population densities.
KW - γ-Aminobutyric acid
KW - Brachionus plicatilis
KW - Food limitation
KW - Free ammonia
KW - Growth hormone
KW - Reproduction
KW - Rotifera
UR - http://www.scopus.com/inward/record.url?scp=0033565360&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033565360&partnerID=8YFLogxK
U2 - 10.1016/S0022-0981(99)00055-6
DO - 10.1016/S0022-0981(99)00055-6
M3 - Article
VL - 240
SP - 179
EP - 191
JO - Journal of Experimental Marine Biology and Ecology
JF - Journal of Experimental Marine Biology and Ecology
SN - 0022-0981
IS - 2
ER -