Effect of growth hormone and γ-aminobutyric acid on Brachionus plicatilis (Rotifera) reproduction at low food or high ammonia levels

Wenresti G. Gallardo, Atsushi Hagiwara, Yuichi Tomita, Terry W. Snell

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Growth hormone (GH, 0.0025 and 0.025 I.U. ml-1) and γ-aminobutyric acid (GABA, 50 μg ml-1) enhance rotifer population growth in batch cultures. In order to further understand the mechanism of their actions, we conducted experiments culturing isolated females at low food and high free ammonia levels. At an optimum food level of 7 x 106 Nannochloropsis oculata cells ml-1 or at low free ammonia level of 2.4 μg ml-1, the F1 offspring of rotifers treated with GH at 0.0025 I.U. ml-1 had significantly higher population growth rate (r) and net reproduction rate (Ro), and shorter generation time than untreated rotifers. At a lower food level of 7 x 105 cells ml-1 or at high free ammonia level of 3.1 μg ml-1, rotifers treated with GABA at 50 μg ml-1 had significantly higher r and Ro, and shorter generation time. These results indicate that GABA is effective in enhancing rotifer reproduction when rotifers are cultured under stress whereas GH enhances rotifer reproduction when culture conditions are optimal. Significant effects were also observed in F1 and F2 generations which were not treated with hormones. These data may be useful for treating rotifer mass cultures to mitigate the effects of stress caused by high population densities.

Original languageEnglish
Pages (from-to)179-191
Number of pages13
JournalJournal of Experimental Marine Biology and Ecology
Volume240
Issue number2
DOIs
Publication statusPublished - Jul 15 1999

Fingerprint

Brachionus plicatilis
Rotifera
somatotropin
hormone
ammonia
generation time
food
population growth
acids
acid
gamma-aminobutyric acid
population density
Nannochloropsis
effect
experiment
mechanism of action
hormones
cells

Keywords

  • γ-Aminobutyric acid
  • Brachionus plicatilis
  • Food limitation
  • Free ammonia
  • Growth hormone
  • Reproduction
  • Rotifera

ASJC Scopus subject areas

  • Aquatic Science
  • Ecology, Evolution, Behavior and Systematics
  • Ecology

Cite this

Effect of growth hormone and γ-aminobutyric acid on Brachionus plicatilis (Rotifera) reproduction at low food or high ammonia levels. / Gallardo, Wenresti G.; Hagiwara, Atsushi; Tomita, Yuichi; Snell, Terry W.

In: Journal of Experimental Marine Biology and Ecology, Vol. 240, No. 2, 15.07.1999, p. 179-191.

Research output: Contribution to journalArticle

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