Effect of formulated zimmu (Allium cepa L. x Allium sativum L.) extract in the management of grain mold of sorghum

Muthusamy Karthikeyan, Rajendran Sandosskumar, Raju Radhajeyalakshmi, Subramanian Mathiyazhagan, Salah Eddin Khabbaz, Kulandaivelu Ganesamurthy, Balasubramanian Selvi, Rethinasamy Velazhahan

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

BACKGROUND: The in vitro antifungal activity of zimmu (Allium cepa L. x Allium sativum L.) extract against Aspergillus flavus Link., Fusarium moniliforme Sheld, Curvularia lunata (Wakker) Boedijn and Alternaria alternata (Fr.) Keissler, the fungi associated with grain mold of sorghum, was evaluated. An emulsifiable concentrate (EC) formulation of zimmu extract was prepared and its efficacy in controlling grain mold was tested under field conditions. RESULTS: The leaf extract of zimmu exhibited strong antifungal activity against A. flavus, F. moniliforme, C. lunata and A. alternata and caused in vitro fungal growth inhibition of 73.3%, 71.1%, 70.0% and 74.4%, respectively. Foliar application of zimmu formulation 50 EC at 3 mLL-1 (v/v) concentration 60, 75 and 90 days after sowing significantly reduced the incidence of grain mold and increased the grain weight and grain hardness. A significant reduction in the anatoxin B1 content in sorghum grains was observed when sorghum plants were sprayed with zimmu formulation 50 EC at 0.3% concentration. Western blot analysis revealed that a thaumatin-like protein (TLP) a molecular mass of 23 kDa that cross-reacted with zeamatin antiserum was expressed at higher levels in sorghum grains after treatment with zimmu formulations. CONCLUSION: The leaf extract of zimmu exhibited antifungal activity against sorghum grain mold fungi. The formulated zimmu extract was significantly effective in reducing the grain mold incidence in sorghum under field conditions. This formulated zimmu extract offers a novel alternative for eco-friendly management of grain mold of sorghum.

Original languageEnglish
Pages (from-to)2495-2501
Number of pages7
JournalJournal of the Science of Food and Agriculture
Volume87
Issue number13
DOIs
Publication statusPublished - Oct 2007

Fingerprint

Garlic
Sorghum
Onions
Allium sativum
Allium cepa
molds (fungi)
Fungi
emulsifiable concentrates
extracts
grain sorghum
Alternaria alternata
Aspergillus flavus
leaf extracts
anatoxins
incidence
fungi
Alternaria
foliar application
Incidence
Hardness

Keywords

  • Botanicals
  • Formulation
  • Grain mold
  • Sorghum bicolor

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Agronomy and Crop Science
  • Nutrition and Dietetics

Cite this

Effect of formulated zimmu (Allium cepa L. x Allium sativum L.) extract in the management of grain mold of sorghum. / Karthikeyan, Muthusamy; Sandosskumar, Rajendran; Radhajeyalakshmi, Raju; Mathiyazhagan, Subramanian; Khabbaz, Salah Eddin; Ganesamurthy, Kulandaivelu; Selvi, Balasubramanian; Velazhahan, Rethinasamy.

In: Journal of the Science of Food and Agriculture, Vol. 87, No. 13, 10.2007, p. 2495-2501.

Research output: Contribution to journalArticle

Karthikeyan, M, Sandosskumar, R, Radhajeyalakshmi, R, Mathiyazhagan, S, Khabbaz, SE, Ganesamurthy, K, Selvi, B & Velazhahan, R 2007, 'Effect of formulated zimmu (Allium cepa L. x Allium sativum L.) extract in the management of grain mold of sorghum', Journal of the Science of Food and Agriculture, vol. 87, no. 13, pp. 2495-2501. https://doi.org/10.1002/jsfa.3010
Karthikeyan M, Sandosskumar R, Radhajeyalakshmi R, Mathiyazhagan S, Khabbaz SE, Ganesamurthy K et al. Effect of formulated zimmu (Allium cepa L. x Allium sativum L.) extract in the management of grain mold of sorghum. Journal of the Science of Food and Agriculture. 2007 Oct;87(13):2495-2501. https://doi.org/10.1002/jsfa.3010
Karthikeyan, Muthusamy ; Sandosskumar, Rajendran ; Radhajeyalakshmi, Raju ; Mathiyazhagan, Subramanian ; Khabbaz, Salah Eddin ; Ganesamurthy, Kulandaivelu ; Selvi, Balasubramanian ; Velazhahan, Rethinasamy. / Effect of formulated zimmu (Allium cepa L. x Allium sativum L.) extract in the management of grain mold of sorghum. In: Journal of the Science of Food and Agriculture. 2007 ; Vol. 87, No. 13. pp. 2495-2501.
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abstract = "BACKGROUND: The in vitro antifungal activity of zimmu (Allium cepa L. x Allium sativum L.) extract against Aspergillus flavus Link., Fusarium moniliforme Sheld, Curvularia lunata (Wakker) Boedijn and Alternaria alternata (Fr.) Keissler, the fungi associated with grain mold of sorghum, was evaluated. An emulsifiable concentrate (EC) formulation of zimmu extract was prepared and its efficacy in controlling grain mold was tested under field conditions. RESULTS: The leaf extract of zimmu exhibited strong antifungal activity against A. flavus, F. moniliforme, C. lunata and A. alternata and caused in vitro fungal growth inhibition of 73.3{\%}, 71.1{\%}, 70.0{\%} and 74.4{\%}, respectively. Foliar application of zimmu formulation 50 EC at 3 mLL-1 (v/v) concentration 60, 75 and 90 days after sowing significantly reduced the incidence of grain mold and increased the grain weight and grain hardness. A significant reduction in the anatoxin B1 content in sorghum grains was observed when sorghum plants were sprayed with zimmu formulation 50 EC at 0.3{\%} concentration. Western blot analysis revealed that a thaumatin-like protein (TLP) a molecular mass of 23 kDa that cross-reacted with zeamatin antiserum was expressed at higher levels in sorghum grains after treatment with zimmu formulations. CONCLUSION: The leaf extract of zimmu exhibited antifungal activity against sorghum grain mold fungi. The formulated zimmu extract was significantly effective in reducing the grain mold incidence in sorghum under field conditions. This formulated zimmu extract offers a novel alternative for eco-friendly management of grain mold of sorghum.",
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AU - Radhajeyalakshmi, Raju

AU - Mathiyazhagan, Subramanian

AU - Khabbaz, Salah Eddin

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AB - BACKGROUND: The in vitro antifungal activity of zimmu (Allium cepa L. x Allium sativum L.) extract against Aspergillus flavus Link., Fusarium moniliforme Sheld, Curvularia lunata (Wakker) Boedijn and Alternaria alternata (Fr.) Keissler, the fungi associated with grain mold of sorghum, was evaluated. An emulsifiable concentrate (EC) formulation of zimmu extract was prepared and its efficacy in controlling grain mold was tested under field conditions. RESULTS: The leaf extract of zimmu exhibited strong antifungal activity against A. flavus, F. moniliforme, C. lunata and A. alternata and caused in vitro fungal growth inhibition of 73.3%, 71.1%, 70.0% and 74.4%, respectively. Foliar application of zimmu formulation 50 EC at 3 mLL-1 (v/v) concentration 60, 75 and 90 days after sowing significantly reduced the incidence of grain mold and increased the grain weight and grain hardness. A significant reduction in the anatoxin B1 content in sorghum grains was observed when sorghum plants were sprayed with zimmu formulation 50 EC at 0.3% concentration. Western blot analysis revealed that a thaumatin-like protein (TLP) a molecular mass of 23 kDa that cross-reacted with zeamatin antiserum was expressed at higher levels in sorghum grains after treatment with zimmu formulations. CONCLUSION: The leaf extract of zimmu exhibited antifungal activity against sorghum grain mold fungi. The formulated zimmu extract was significantly effective in reducing the grain mold incidence in sorghum under field conditions. This formulated zimmu extract offers a novel alternative for eco-friendly management of grain mold of sorghum.

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