Effect of fluoxetine and resveratrol on testicular functions and oxidative stress in a rat model of chronic mild stress-induced depression

Hussein F. Sakr, A. M. Abbas, A. Z. Elsamanoudy, F. M. Ghoneim

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Our objective was to investigate the effects of chronic unpredictable mild stress (CUMS) with or without selective serotonin reuptake inhibitor (fluoxetine) and anti-oxidant (resveratrol) on testicular functions and oxidative stress in rats. Fifty male rats were divided into 2 groups; control and CUMS. CUMS group was further subdivided into 4 subgroups administered water, fluoxetine, resveratrol and both. Sucrose intake, body weight gain, serum corticosterone, serotonin and testosterone levels, sperm count and motility, testicular malondialdehyde, superoxide dismutase (SOD), catalase, glutathione (GSH), and gene expression of steroidogenic acute-regulatory (StAR) protein and cytochrome P450 side chain cleavage (P450scc) enzyme were evaluated. CUMS decreased sucrose intake, weight gain, anti-oxidants (SOD, catalase, GSH), testosterone, serotonin, StAR and cytochrome P450scc gene expression, sperm count and motility and increased malondialdehyde and corticosterone. Fluoxetine increased malondialdehyde, sucrose intake, weight gain, serotonin and decreased anti-oxidants, StAR and cytochrome P450scc gene expression, sperm count and motility, testosterone, corticosterone in stressed rats. Administration of resveratrol increased anti-oxidants, sucrose intake, weight gain, serotonin, StAR and cytochrome P450scc gene expression, testosterone, sperm count and motility, and decreased malondialdehyde and corticosterone in stressed rats with or without fluoxetine. In conclusion, CUMS induces testicular dysfunctions and oxidative stress. While treatment of CUMS rats with fluoxetine decreases the depressive behavior, it causes further worsening of testicular dysfunctions and oxidative stress. Administration of resveratrol improves testicular dysfunctions and oxidative stress that are caused by CUMS and further worsened by fluoxetine treatment.

Original languageEnglish
Pages (from-to)515-527
Number of pages13
JournalJournal of Physiology and Pharmacology
Volume66
Issue number4
Publication statusPublished - Aug 1 2015

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Fluoxetine
Oxidative Stress
Sperm Count
Sperm Motility
Depression
Corticosterone
Malondialdehyde
Oxidants
Cytochrome P-450 Enzyme System
Weight Gain
Sucrose
Testosterone
Serotonin
Gene Expression
Catalase
Superoxide Dismutase
Serotonin Uptake Inhibitors
Glutathione
resveratrol
Body Weight

Keywords

  • Chronic mild stress
  • Depression
  • Fluoxetine
  • Oxidative stress
  • Resveratrol
  • Serotonin
  • Sucrose intake
  • Testicular functions

ASJC Scopus subject areas

  • Physiology
  • Pharmacology

Cite this

Effect of fluoxetine and resveratrol on testicular functions and oxidative stress in a rat model of chronic mild stress-induced depression. / Sakr, Hussein F.; Abbas, A. M.; Elsamanoudy, A. Z.; Ghoneim, F. M.

In: Journal of Physiology and Pharmacology, Vol. 66, No. 4, 01.08.2015, p. 515-527.

Research output: Contribution to journalArticle

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