Effect of bacteria associated with the green alga Ulva reticulata on marine micro- and macrofouling

Sergey V. Dobretsov, Pei Yuan Qian

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

The green alga Ulva reticulata (Forsskal) is often free from biofouling in Hong Kong waters. An early study indicated that bioactive substances from this alga inhibit settlement of the polychaete Hydroides elegans (Haswell). It is also predicted that epibiotic bacteria protect this alga from micro- and macrofouling. In this study, bacterial strains from the surface of U. reticulata were isolated and their inhibitive activities on micro- and macrofouling assayed. The strains were identified by 16S rRNA analysis as belonging to the genera Alteromonas, Pseudoalteromonas and Vibrio. There was no significant effect of these strains or their extracts (aqueous and ethanol) on the growth of five Vibrio strains isolated from natural biofilm. Two bacterial strains (Alteromonas sp. and Vibrio sp. 3) were non-toxic to the benthic diatom Nitzschia paleacea (Grunow) while the other five strains caused a low level of mortality. No one bacterial strain was toxic to the larvae of H. elegans. Aqueous extract of one of the isolated bacterial species, i.e. Vibrio sp. 2, significantly (p <0.00001) inhibited the settlement and metamorphosis of H. elegans larvae. The putative antifouling compounds have a molecular weight of > 100kD. On the other hand, biofilm of Pseudoalteromonas sp. 2 and aqueous extract of Vibrio sp. 2 suppressed the settlement of larvae induced by 3-isobutyl-1-methylxanthine (IBMX). Other epibiotic bacteria and their extracts had neither inhibitive nor inductive effects on larval settlement of H. elegans. The results indicate that the antifouling mechanism of U. reticulata may be dependent not only on materials from the macroalga itself but also on the epibiotic bacteria on the algal surface.

Original languageEnglish
Pages (from-to)217-228
Number of pages12
JournalBiofouling
Volume18
Issue number3
DOIs
Publication statusPublished - 2002

Fingerprint

Ulva
Chlorophyta
Vibrio
green alga
Bacteria
Alteromonas
Pseudoalteromonas
bacterium
biofilm
bacteria
algae
alga
larva
larval settlement
Biofilms
macroalga
biofouling
antifouling
Larva
polychaete

Keywords

  • Biofilm
  • Biofouling
  • Epibiotic bacteria
  • Larval metamorphosis
  • Larval settlement
  • Ulva reticulata

ASJC Scopus subject areas

  • Aquatic Science
  • Biotechnology

Cite this

Effect of bacteria associated with the green alga Ulva reticulata on marine micro- and macrofouling. / Dobretsov, Sergey V.; Qian, Pei Yuan.

In: Biofouling, Vol. 18, No. 3, 2002, p. 217-228.

Research output: Contribution to journalArticle

@article{11f07f2788dc4f9ea5cea9f9a6560637,
title = "Effect of bacteria associated with the green alga Ulva reticulata on marine micro- and macrofouling",
abstract = "The green alga Ulva reticulata (Forsskal) is often free from biofouling in Hong Kong waters. An early study indicated that bioactive substances from this alga inhibit settlement of the polychaete Hydroides elegans (Haswell). It is also predicted that epibiotic bacteria protect this alga from micro- and macrofouling. In this study, bacterial strains from the surface of U. reticulata were isolated and their inhibitive activities on micro- and macrofouling assayed. The strains were identified by 16S rRNA analysis as belonging to the genera Alteromonas, Pseudoalteromonas and Vibrio. There was no significant effect of these strains or their extracts (aqueous and ethanol) on the growth of five Vibrio strains isolated from natural biofilm. Two bacterial strains (Alteromonas sp. and Vibrio sp. 3) were non-toxic to the benthic diatom Nitzschia paleacea (Grunow) while the other five strains caused a low level of mortality. No one bacterial strain was toxic to the larvae of H. elegans. Aqueous extract of one of the isolated bacterial species, i.e. Vibrio sp. 2, significantly (p <0.00001) inhibited the settlement and metamorphosis of H. elegans larvae. The putative antifouling compounds have a molecular weight of > 100kD. On the other hand, biofilm of Pseudoalteromonas sp. 2 and aqueous extract of Vibrio sp. 2 suppressed the settlement of larvae induced by 3-isobutyl-1-methylxanthine (IBMX). Other epibiotic bacteria and their extracts had neither inhibitive nor inductive effects on larval settlement of H. elegans. The results indicate that the antifouling mechanism of U. reticulata may be dependent not only on materials from the macroalga itself but also on the epibiotic bacteria on the algal surface.",
keywords = "Biofilm, Biofouling, Epibiotic bacteria, Larval metamorphosis, Larval settlement, Ulva reticulata",
author = "Dobretsov, {Sergey V.} and Qian, {Pei Yuan}",
year = "2002",
doi = "10.1080/08927010290013026",
language = "English",
volume = "18",
pages = "217--228",
journal = "Biofouling",
issn = "0892-7014",
publisher = "Taylor and Francis Ltd.",
number = "3",

}

TY - JOUR

T1 - Effect of bacteria associated with the green alga Ulva reticulata on marine micro- and macrofouling

AU - Dobretsov, Sergey V.

AU - Qian, Pei Yuan

PY - 2002

Y1 - 2002

N2 - The green alga Ulva reticulata (Forsskal) is often free from biofouling in Hong Kong waters. An early study indicated that bioactive substances from this alga inhibit settlement of the polychaete Hydroides elegans (Haswell). It is also predicted that epibiotic bacteria protect this alga from micro- and macrofouling. In this study, bacterial strains from the surface of U. reticulata were isolated and their inhibitive activities on micro- and macrofouling assayed. The strains were identified by 16S rRNA analysis as belonging to the genera Alteromonas, Pseudoalteromonas and Vibrio. There was no significant effect of these strains or their extracts (aqueous and ethanol) on the growth of five Vibrio strains isolated from natural biofilm. Two bacterial strains (Alteromonas sp. and Vibrio sp. 3) were non-toxic to the benthic diatom Nitzschia paleacea (Grunow) while the other five strains caused a low level of mortality. No one bacterial strain was toxic to the larvae of H. elegans. Aqueous extract of one of the isolated bacterial species, i.e. Vibrio sp. 2, significantly (p <0.00001) inhibited the settlement and metamorphosis of H. elegans larvae. The putative antifouling compounds have a molecular weight of > 100kD. On the other hand, biofilm of Pseudoalteromonas sp. 2 and aqueous extract of Vibrio sp. 2 suppressed the settlement of larvae induced by 3-isobutyl-1-methylxanthine (IBMX). Other epibiotic bacteria and their extracts had neither inhibitive nor inductive effects on larval settlement of H. elegans. The results indicate that the antifouling mechanism of U. reticulata may be dependent not only on materials from the macroalga itself but also on the epibiotic bacteria on the algal surface.

AB - The green alga Ulva reticulata (Forsskal) is often free from biofouling in Hong Kong waters. An early study indicated that bioactive substances from this alga inhibit settlement of the polychaete Hydroides elegans (Haswell). It is also predicted that epibiotic bacteria protect this alga from micro- and macrofouling. In this study, bacterial strains from the surface of U. reticulata were isolated and their inhibitive activities on micro- and macrofouling assayed. The strains were identified by 16S rRNA analysis as belonging to the genera Alteromonas, Pseudoalteromonas and Vibrio. There was no significant effect of these strains or their extracts (aqueous and ethanol) on the growth of five Vibrio strains isolated from natural biofilm. Two bacterial strains (Alteromonas sp. and Vibrio sp. 3) were non-toxic to the benthic diatom Nitzschia paleacea (Grunow) while the other five strains caused a low level of mortality. No one bacterial strain was toxic to the larvae of H. elegans. Aqueous extract of one of the isolated bacterial species, i.e. Vibrio sp. 2, significantly (p <0.00001) inhibited the settlement and metamorphosis of H. elegans larvae. The putative antifouling compounds have a molecular weight of > 100kD. On the other hand, biofilm of Pseudoalteromonas sp. 2 and aqueous extract of Vibrio sp. 2 suppressed the settlement of larvae induced by 3-isobutyl-1-methylxanthine (IBMX). Other epibiotic bacteria and their extracts had neither inhibitive nor inductive effects on larval settlement of H. elegans. The results indicate that the antifouling mechanism of U. reticulata may be dependent not only on materials from the macroalga itself but also on the epibiotic bacteria on the algal surface.

KW - Biofilm

KW - Biofouling

KW - Epibiotic bacteria

KW - Larval metamorphosis

KW - Larval settlement

KW - Ulva reticulata

UR - http://www.scopus.com/inward/record.url?scp=0036066977&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036066977&partnerID=8YFLogxK

U2 - 10.1080/08927010290013026

DO - 10.1080/08927010290013026

M3 - Article

AN - SCOPUS:0036066977

VL - 18

SP - 217

EP - 228

JO - Biofouling

JF - Biofouling

SN - 0892-7014

IS - 3

ER -