Diversity of tetracycline resistant genes in Escherichia coli from human and environmental sources

Saif Al-Bahry, Nawal Al-Sharji, Mahmoud Yaish, Salma Al-Musharafi, Ibrahim Mahmoud

Research output: Contribution to journalArticle

Abstract

Worldwide tetracycline resistance (Tcr) is increasing dramatically,causing serious environmental and health problems. A total of 201 samples were collected from chicken intestine,human feces and treated sewage effluent (TSE). One hundred and eighteen Escherichia coli strains were isolated and identified using MALDI-Biotyper. Single and multiplex PCR were used to screen isolates for 14 tet genes,among which only 7 tet genes (A,B,C,M,Q,W,32) were found. Among the resistant isolates,tet A was the most frequent gene,followed by tet B and tet 32 while the rest of tet determinants occurred at a lower frequency. Many strains contained multiple Tcr determinants. Some strains contained 4 tet gene-combination,tet (A/B/C/32) and tet (A/B/M/32). The 4 tet gene combination is reported for the first time in this region. The Tcr isolates showed a high variation of tet gene combination. The increase in the resistance of tetracycline with high diversification is an indication of antibiotics overuse. Strict enforcement of regulation is urgently needed to control and prevent the spread of tetracycline resistant strains which are detrimental to the environment.

Original languageEnglish
Pages (from-to)289-300
Number of pages12
JournalOpen Biotechnology Journal
Volume10
DOIs
Publication statusPublished - 2016

Fingerprint

Tetracycline
Escherichia coli
Tetracycline Resistance
Genes
Environmental Health
Multiplex Polymerase Chain Reaction
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Antibiotics
Sewage
Medical problems
Feces
Intestines
Effluents
Chickens
Anti-Bacterial Agents

Keywords

  • Chicken
  • Environment
  • Escherichia coli
  • Maldi-biotyper
  • Tetracycline-resistance
  • Treated sewage effluent

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biophysics
  • Biochemistry
  • Biomedical Engineering

Cite this

Diversity of tetracycline resistant genes in Escherichia coli from human and environmental sources. / Al-Bahry, Saif; Al-Sharji, Nawal; Yaish, Mahmoud; Al-Musharafi, Salma; Mahmoud, Ibrahim.

In: Open Biotechnology Journal, Vol. 10, 2016, p. 289-300.

Research output: Contribution to journalArticle

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