Diversity of Bacterial Communities Along a Petroleum Contamination Gradient in Desert Soils

Raeid M M Abed, Sumaiya Al-Kindi, Samiha Al-Kharusi

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Microbial communities in oil-polluted desert soils have been rarely studied compared to their counterparts from freshwater and marine environments. We investigated bacterial diversity and changes therein in five desert soils exposed to different levels of oil pollution. Automated rRNA intergenic spacer (ARISA) analysis profiles showed that the bacterial communities of the five soils were profoundly different (analysis of similarities (ANOSIM), R = 0.45, P <0.0001) and shared less than 20 % of their operational taxonomic units (OTUs). OTU richness was relatively higher in the soils with the higher oil pollution levels. Multivariate analyses of ARISA profiles revealed that the microbial communities in the S soil, which contains the highest level of contamination, were different from the other soils and formed a completely separate cluster. A total of 16,657 ribosomal sequences were obtained, with 42–89 % of these sequences belonging to the phylum Proteobacteria. While sequences belonging to Betaproteobacteria, Gammaproteobacteria, Bacilli, and Actinobacteria were encountered in all soils, sequences belonging to anaerobic bacteria from the classes Deltaproteobacteria, Clostridia, and Anaerolineae were only detected in the S soil. Sequences belonging to the genus Terriglobus of the class Acidobacteria were only detected in the B3 soil with the lowest level of contamination. Redundancy analysis (RDA) showed that oil contamination level was the most determinant factor that explained variations in the microbial communities. We conclude that the exposure to different levels of oil contamination exerts a strong selective pressure on bacterial communities and that desert soils are rich in aerobic and anaerobic bacteria that could potentially contribute to the degradation of hydrocarbons.

Original languageEnglish
Pages (from-to)95-105
Number of pages11
JournalMicrobial Ecology
Volume69
Issue number1
DOIs
Publication statusPublished - 2014

Fingerprint

desert soils
desert soil
Petroleum
bacterial communities
petroleum
Soil
oils
microbial communities
soil
intergenic DNA
microbial community
oil pollution
pollution
ribosomal RNA
soil sequences
Petroleum Pollution
delta-Proteobacteria
Acidobacteria
Oils
oil

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Soil Science
  • Medicine(all)

Cite this

Diversity of Bacterial Communities Along a Petroleum Contamination Gradient in Desert Soils. / Abed, Raeid M M; Al-Kindi, Sumaiya; Al-Kharusi, Samiha.

In: Microbial Ecology, Vol. 69, No. 1, 2014, p. 95-105.

Research output: Contribution to journalArticle

Abed, Raeid M M ; Al-Kindi, Sumaiya ; Al-Kharusi, Samiha. / Diversity of Bacterial Communities Along a Petroleum Contamination Gradient in Desert Soils. In: Microbial Ecology. 2014 ; Vol. 69, No. 1. pp. 95-105.
@article{990caacd3ae0482fbd7ba19c896b7d71,
title = "Diversity of Bacterial Communities Along a Petroleum Contamination Gradient in Desert Soils",
abstract = "Microbial communities in oil-polluted desert soils have been rarely studied compared to their counterparts from freshwater and marine environments. We investigated bacterial diversity and changes therein in five desert soils exposed to different levels of oil pollution. Automated rRNA intergenic spacer (ARISA) analysis profiles showed that the bacterial communities of the five soils were profoundly different (analysis of similarities (ANOSIM), R = 0.45, P <0.0001) and shared less than 20 {\%} of their operational taxonomic units (OTUs). OTU richness was relatively higher in the soils with the higher oil pollution levels. Multivariate analyses of ARISA profiles revealed that the microbial communities in the S soil, which contains the highest level of contamination, were different from the other soils and formed a completely separate cluster. A total of 16,657 ribosomal sequences were obtained, with 42–89 {\%} of these sequences belonging to the phylum Proteobacteria. While sequences belonging to Betaproteobacteria, Gammaproteobacteria, Bacilli, and Actinobacteria were encountered in all soils, sequences belonging to anaerobic bacteria from the classes Deltaproteobacteria, Clostridia, and Anaerolineae were only detected in the S soil. Sequences belonging to the genus Terriglobus of the class Acidobacteria were only detected in the B3 soil with the lowest level of contamination. Redundancy analysis (RDA) showed that oil contamination level was the most determinant factor that explained variations in the microbial communities. We conclude that the exposure to different levels of oil contamination exerts a strong selective pressure on bacterial communities and that desert soils are rich in aerobic and anaerobic bacteria that could potentially contribute to the degradation of hydrocarbons.",
author = "Abed, {Raeid M M} and Sumaiya Al-Kindi and Samiha Al-Kharusi",
year = "2014",
doi = "10.1007/s00248-014-0475-5",
language = "English",
volume = "69",
pages = "95--105",
journal = "Microbial Ecology",
issn = "0095-3628",
publisher = "Springer New York",
number = "1",

}

TY - JOUR

T1 - Diversity of Bacterial Communities Along a Petroleum Contamination Gradient in Desert Soils

AU - Abed, Raeid M M

AU - Al-Kindi, Sumaiya

AU - Al-Kharusi, Samiha

PY - 2014

Y1 - 2014

N2 - Microbial communities in oil-polluted desert soils have been rarely studied compared to their counterparts from freshwater and marine environments. We investigated bacterial diversity and changes therein in five desert soils exposed to different levels of oil pollution. Automated rRNA intergenic spacer (ARISA) analysis profiles showed that the bacterial communities of the five soils were profoundly different (analysis of similarities (ANOSIM), R = 0.45, P <0.0001) and shared less than 20 % of their operational taxonomic units (OTUs). OTU richness was relatively higher in the soils with the higher oil pollution levels. Multivariate analyses of ARISA profiles revealed that the microbial communities in the S soil, which contains the highest level of contamination, were different from the other soils and formed a completely separate cluster. A total of 16,657 ribosomal sequences were obtained, with 42–89 % of these sequences belonging to the phylum Proteobacteria. While sequences belonging to Betaproteobacteria, Gammaproteobacteria, Bacilli, and Actinobacteria were encountered in all soils, sequences belonging to anaerobic bacteria from the classes Deltaproteobacteria, Clostridia, and Anaerolineae were only detected in the S soil. Sequences belonging to the genus Terriglobus of the class Acidobacteria were only detected in the B3 soil with the lowest level of contamination. Redundancy analysis (RDA) showed that oil contamination level was the most determinant factor that explained variations in the microbial communities. We conclude that the exposure to different levels of oil contamination exerts a strong selective pressure on bacterial communities and that desert soils are rich in aerobic and anaerobic bacteria that could potentially contribute to the degradation of hydrocarbons.

AB - Microbial communities in oil-polluted desert soils have been rarely studied compared to their counterparts from freshwater and marine environments. We investigated bacterial diversity and changes therein in five desert soils exposed to different levels of oil pollution. Automated rRNA intergenic spacer (ARISA) analysis profiles showed that the bacterial communities of the five soils were profoundly different (analysis of similarities (ANOSIM), R = 0.45, P <0.0001) and shared less than 20 % of their operational taxonomic units (OTUs). OTU richness was relatively higher in the soils with the higher oil pollution levels. Multivariate analyses of ARISA profiles revealed that the microbial communities in the S soil, which contains the highest level of contamination, were different from the other soils and formed a completely separate cluster. A total of 16,657 ribosomal sequences were obtained, with 42–89 % of these sequences belonging to the phylum Proteobacteria. While sequences belonging to Betaproteobacteria, Gammaproteobacteria, Bacilli, and Actinobacteria were encountered in all soils, sequences belonging to anaerobic bacteria from the classes Deltaproteobacteria, Clostridia, and Anaerolineae were only detected in the S soil. Sequences belonging to the genus Terriglobus of the class Acidobacteria were only detected in the B3 soil with the lowest level of contamination. Redundancy analysis (RDA) showed that oil contamination level was the most determinant factor that explained variations in the microbial communities. We conclude that the exposure to different levels of oil contamination exerts a strong selective pressure on bacterial communities and that desert soils are rich in aerobic and anaerobic bacteria that could potentially contribute to the degradation of hydrocarbons.

UR - http://www.scopus.com/inward/record.url?scp=84937632933&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84937632933&partnerID=8YFLogxK

U2 - 10.1007/s00248-014-0475-5

DO - 10.1007/s00248-014-0475-5

M3 - Article

C2 - 25103912

AN - SCOPUS:84937632933

VL - 69

SP - 95

EP - 105

JO - Microbial Ecology

JF - Microbial Ecology

SN - 0095-3628

IS - 1

ER -