Dinitrogen-fixing cyanobacteria in microbial mats of two shallow coral reef ecosystems

Loic Charpy, Katarzyna A. Palinska, Beatriz Casareto, Marie José Langlade, Yoshimi Suzuki, Raeid M M Abed, Stjepko Golubic

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Dinitrogen-fixing organisms in cyanobacterial mats were studied in two shallow coral reef ecosystems: La Reunion Island, southwestern Indian Ocean, Sesoko (Okinawa) Island, and northwestern Pacific Ocean. Rapidly expanding benthic miniblooms, frequently dominated by a single cyanobacterial taxon, were identified by microscopy and molecular tools. In addition, nitrogenase activity by these blooms was measured in situ. Dinitrogen fixation and its contribution to mat primary production were calculated using 15N2 and 13C methods. Dinitrogen-fixing cyanobacteria from mats in La Reunion and Sesoko showed few differences in taxonomic composition. Anabaena sp. among heterocystous and Hydrocoleum majus and Symploca hydnoides among nonheterocystous cyanobacteria occurred in microbial mats of both sites. Oscillatoria bonnemaisonii and Leptolyngbya spp. occurred only in La Reunion, whereas Hydrocoleum coccineum dominated in Sesoko. Other mats dominated by Hydrocoleum lyngbyaceum, Phormidium laysanense, and Trichocoleus tenerrimus occurred at lower frequencies. The 24-h nitrogenase activity, as measured by acetylene reduction, varied between 11 and 324 nmoles C2H2 reduced μg-1 Chl a. The highest values were achieved by heterocystous Anabaena sp. performed mostly during the day. Highest values for nonheterocystous cyanobacteria were achieved by H. coccineum mostly during the night. Daily nitrogen fixation varied from nine (Leptolyngbya) to 238 nmoles N2 μg-1 Chl day-1 (H. coccineum). Primary production rates ranged from 1,321 (S. hydnoides) to 9,933 nmoles C μg-1 Chl day-1 (H. coccineum). Dinitrogen fixation satisfied between 5% and 21% of the nitrogen required for primary production.

Original languageEnglish
Pages (from-to)174-186
Number of pages13
JournalMicrobial Ecology
Volume59
Issue number1
DOIs
Publication statusPublished - Jan 2010

Fingerprint

Reunion
microbial mat
Leptolyngbya
nitrogen fixation
coral reefs
coral reef
primary production
primary productivity
Cyanobacteria
cyanobacterium
Anabaena
nitrogenase
fixation
ecosystems
ecosystem
Symploca
nitrogen
Phormidium
Oscillatoria
acetylene reduction

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Soil Science

Cite this

Charpy, L., Palinska, K. A., Casareto, B., Langlade, M. J., Suzuki, Y., Abed, R. M. M., & Golubic, S. (2010). Dinitrogen-fixing cyanobacteria in microbial mats of two shallow coral reef ecosystems. Microbial Ecology, 59(1), 174-186. https://doi.org/10.1007/s00248-009-9576-y

Dinitrogen-fixing cyanobacteria in microbial mats of two shallow coral reef ecosystems. / Charpy, Loic; Palinska, Katarzyna A.; Casareto, Beatriz; Langlade, Marie José; Suzuki, Yoshimi; Abed, Raeid M M; Golubic, Stjepko.

In: Microbial Ecology, Vol. 59, No. 1, 01.2010, p. 174-186.

Research output: Contribution to journalArticle

Charpy, L, Palinska, KA, Casareto, B, Langlade, MJ, Suzuki, Y, Abed, RMM & Golubic, S 2010, 'Dinitrogen-fixing cyanobacteria in microbial mats of two shallow coral reef ecosystems', Microbial Ecology, vol. 59, no. 1, pp. 174-186. https://doi.org/10.1007/s00248-009-9576-y
Charpy, Loic ; Palinska, Katarzyna A. ; Casareto, Beatriz ; Langlade, Marie José ; Suzuki, Yoshimi ; Abed, Raeid M M ; Golubic, Stjepko. / Dinitrogen-fixing cyanobacteria in microbial mats of two shallow coral reef ecosystems. In: Microbial Ecology. 2010 ; Vol. 59, No. 1. pp. 174-186.
@article{7705ef1ba9e4468aafd323d4cdbe05d4,
title = "Dinitrogen-fixing cyanobacteria in microbial mats of two shallow coral reef ecosystems",
abstract = "Dinitrogen-fixing organisms in cyanobacterial mats were studied in two shallow coral reef ecosystems: La Reunion Island, southwestern Indian Ocean, Sesoko (Okinawa) Island, and northwestern Pacific Ocean. Rapidly expanding benthic miniblooms, frequently dominated by a single cyanobacterial taxon, were identified by microscopy and molecular tools. In addition, nitrogenase activity by these blooms was measured in situ. Dinitrogen fixation and its contribution to mat primary production were calculated using 15N2 and 13C methods. Dinitrogen-fixing cyanobacteria from mats in La Reunion and Sesoko showed few differences in taxonomic composition. Anabaena sp. among heterocystous and Hydrocoleum majus and Symploca hydnoides among nonheterocystous cyanobacteria occurred in microbial mats of both sites. Oscillatoria bonnemaisonii and Leptolyngbya spp. occurred only in La Reunion, whereas Hydrocoleum coccineum dominated in Sesoko. Other mats dominated by Hydrocoleum lyngbyaceum, Phormidium laysanense, and Trichocoleus tenerrimus occurred at lower frequencies. The 24-h nitrogenase activity, as measured by acetylene reduction, varied between 11 and 324 nmoles C2H2 reduced μg-1 Chl a. The highest values were achieved by heterocystous Anabaena sp. performed mostly during the day. Highest values for nonheterocystous cyanobacteria were achieved by H. coccineum mostly during the night. Daily nitrogen fixation varied from nine (Leptolyngbya) to 238 nmoles N2 μg-1 Chl day-1 (H. coccineum). Primary production rates ranged from 1,321 (S. hydnoides) to 9,933 nmoles C μg-1 Chl day-1 (H. coccineum). Dinitrogen fixation satisfied between 5{\%} and 21{\%} of the nitrogen required for primary production.",
author = "Loic Charpy and Palinska, {Katarzyna A.} and Beatriz Casareto and Langlade, {Marie Jos{\'e}} and Yoshimi Suzuki and Abed, {Raeid M M} and Stjepko Golubic",
year = "2010",
month = "1",
doi = "10.1007/s00248-009-9576-y",
language = "English",
volume = "59",
pages = "174--186",
journal = "Microbial Ecology",
issn = "0095-3628",
publisher = "Springer New York",
number = "1",

}

TY - JOUR

T1 - Dinitrogen-fixing cyanobacteria in microbial mats of two shallow coral reef ecosystems

AU - Charpy, Loic

AU - Palinska, Katarzyna A.

AU - Casareto, Beatriz

AU - Langlade, Marie José

AU - Suzuki, Yoshimi

AU - Abed, Raeid M M

AU - Golubic, Stjepko

PY - 2010/1

Y1 - 2010/1

N2 - Dinitrogen-fixing organisms in cyanobacterial mats were studied in two shallow coral reef ecosystems: La Reunion Island, southwestern Indian Ocean, Sesoko (Okinawa) Island, and northwestern Pacific Ocean. Rapidly expanding benthic miniblooms, frequently dominated by a single cyanobacterial taxon, were identified by microscopy and molecular tools. In addition, nitrogenase activity by these blooms was measured in situ. Dinitrogen fixation and its contribution to mat primary production were calculated using 15N2 and 13C methods. Dinitrogen-fixing cyanobacteria from mats in La Reunion and Sesoko showed few differences in taxonomic composition. Anabaena sp. among heterocystous and Hydrocoleum majus and Symploca hydnoides among nonheterocystous cyanobacteria occurred in microbial mats of both sites. Oscillatoria bonnemaisonii and Leptolyngbya spp. occurred only in La Reunion, whereas Hydrocoleum coccineum dominated in Sesoko. Other mats dominated by Hydrocoleum lyngbyaceum, Phormidium laysanense, and Trichocoleus tenerrimus occurred at lower frequencies. The 24-h nitrogenase activity, as measured by acetylene reduction, varied between 11 and 324 nmoles C2H2 reduced μg-1 Chl a. The highest values were achieved by heterocystous Anabaena sp. performed mostly during the day. Highest values for nonheterocystous cyanobacteria were achieved by H. coccineum mostly during the night. Daily nitrogen fixation varied from nine (Leptolyngbya) to 238 nmoles N2 μg-1 Chl day-1 (H. coccineum). Primary production rates ranged from 1,321 (S. hydnoides) to 9,933 nmoles C μg-1 Chl day-1 (H. coccineum). Dinitrogen fixation satisfied between 5% and 21% of the nitrogen required for primary production.

AB - Dinitrogen-fixing organisms in cyanobacterial mats were studied in two shallow coral reef ecosystems: La Reunion Island, southwestern Indian Ocean, Sesoko (Okinawa) Island, and northwestern Pacific Ocean. Rapidly expanding benthic miniblooms, frequently dominated by a single cyanobacterial taxon, were identified by microscopy and molecular tools. In addition, nitrogenase activity by these blooms was measured in situ. Dinitrogen fixation and its contribution to mat primary production were calculated using 15N2 and 13C methods. Dinitrogen-fixing cyanobacteria from mats in La Reunion and Sesoko showed few differences in taxonomic composition. Anabaena sp. among heterocystous and Hydrocoleum majus and Symploca hydnoides among nonheterocystous cyanobacteria occurred in microbial mats of both sites. Oscillatoria bonnemaisonii and Leptolyngbya spp. occurred only in La Reunion, whereas Hydrocoleum coccineum dominated in Sesoko. Other mats dominated by Hydrocoleum lyngbyaceum, Phormidium laysanense, and Trichocoleus tenerrimus occurred at lower frequencies. The 24-h nitrogenase activity, as measured by acetylene reduction, varied between 11 and 324 nmoles C2H2 reduced μg-1 Chl a. The highest values were achieved by heterocystous Anabaena sp. performed mostly during the day. Highest values for nonheterocystous cyanobacteria were achieved by H. coccineum mostly during the night. Daily nitrogen fixation varied from nine (Leptolyngbya) to 238 nmoles N2 μg-1 Chl day-1 (H. coccineum). Primary production rates ranged from 1,321 (S. hydnoides) to 9,933 nmoles C μg-1 Chl day-1 (H. coccineum). Dinitrogen fixation satisfied between 5% and 21% of the nitrogen required for primary production.

UR - http://www.scopus.com/inward/record.url?scp=74149092105&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=74149092105&partnerID=8YFLogxK

U2 - 10.1007/s00248-009-9576-y

DO - 10.1007/s00248-009-9576-y

M3 - Article

VL - 59

SP - 174

EP - 186

JO - Microbial Ecology

JF - Microbial Ecology

SN - 0095-3628

IS - 1

ER -