Differential induction of chitinase and Β-1,3-glucanase in response to inoculation with a pathogen (Rhizoctonia solani) and a non-pathogen (Pestalotia palmarum)

R. Velazhahan; S. D. Deborah; A. Palaniswami

doi:10.1556/APhyt.36.2001.1-2.9

Differential induction of chitinase and Β-1,3-glucanase in response to inoculation with a pathogen (Rhizoctonia solani) and a non-pathogen (Pestalotia palmarum)

R. Velazhahan^*, S. D. Deborah, A. Palaniswami

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Rice leaf sheaths were inoculated with R. solani (pathogen) and P. palmarum (non-pathogen) and were analyzed for the accumulation of pathogenesis-related (PR) proteins. Inoculation of rice plants with R. solani and P. palmarum resulted in a marked increase in activities of chitinase and β-1.3-glucanase. The levels of both enzymes were higher in incompatible interactions than in compatible interactions. Western blot analysis indicated that two proteins with molecular weights of 33 and 35 kDa cross-reacting with barley chitinase antibody were induced in rice in response to inoculation with R. solani. The appearance of these chitinases was correlated with increase in enzyme activity.

Original language	English
Pages (from-to)	67-74
Number of pages	8
Journal	Acta Phytopathologica et Entomologica Hungarica
Volume	36
Issue number	1-2
DOIs	https://doi.org/10.1556/APhyt.36.2001.1-2.9
Publication status	Published - 2001
Externally published	Yes

Keywords

Compatible interaction
Incompatible interaction
Oryza sativa
PR-proteins
Pestalotia palmarum
Rhizoctonia solani

ASJC Scopus subject areas

Plant Science
Insect Science

Access to Document

10.1556/APhyt.36.2001.1-2.9

Cite this

@article{ae83fdf3bc76483281cafd079bf0704c,

title = "Differential induction of chitinase and Β-1,3-glucanase in response to inoculation with a pathogen (Rhizoctonia solani) and a non-pathogen (Pestalotia palmarum)",

abstract = "Rice leaf sheaths were inoculated with R. solani (pathogen) and P. palmarum (non-pathogen) and were analyzed for the accumulation of pathogenesis-related (PR) proteins. Inoculation of rice plants with R. solani and P. palmarum resulted in a marked increase in activities of chitinase and β-1.3-glucanase. The levels of both enzymes were higher in incompatible interactions than in compatible interactions. Western blot analysis indicated that two proteins with molecular weights of 33 and 35 kDa cross-reacting with barley chitinase antibody were induced in rice in response to inoculation with R. solani. The appearance of these chitinases was correlated with increase in enzyme activity.",

keywords = "Compatible interaction, Incompatible interaction, Oryza sativa, PR-proteins, Pestalotia palmarum, Rhizoctonia solani",

author = "R. Velazhahan and Deborah, {S. D.} and A. Palaniswami",

year = "2001",

doi = "10.1556/APhyt.36.2001.1-2.9",

language = "English",

volume = "36",

pages = "67--74",

journal = "Acta Phytopathologica et Entomologica Hungarica",

issn = "0238-1249",

publisher = "Akademiai Kiado",

number = "1-2",

}

TY - JOUR

T1 - Differential induction of chitinase and Β-1,3-glucanase in response to inoculation with a pathogen (Rhizoctonia solani) and a non-pathogen (Pestalotia palmarum)

AU - Velazhahan, R.

AU - Deborah, S. D.

AU - Palaniswami, A.

PY - 2001

Y1 - 2001

N2 - Rice leaf sheaths were inoculated with R. solani (pathogen) and P. palmarum (non-pathogen) and were analyzed for the accumulation of pathogenesis-related (PR) proteins. Inoculation of rice plants with R. solani and P. palmarum resulted in a marked increase in activities of chitinase and β-1.3-glucanase. The levels of both enzymes were higher in incompatible interactions than in compatible interactions. Western blot analysis indicated that two proteins with molecular weights of 33 and 35 kDa cross-reacting with barley chitinase antibody were induced in rice in response to inoculation with R. solani. The appearance of these chitinases was correlated with increase in enzyme activity.

AB - Rice leaf sheaths were inoculated with R. solani (pathogen) and P. palmarum (non-pathogen) and were analyzed for the accumulation of pathogenesis-related (PR) proteins. Inoculation of rice plants with R. solani and P. palmarum resulted in a marked increase in activities of chitinase and β-1.3-glucanase. The levels of both enzymes were higher in incompatible interactions than in compatible interactions. Western blot analysis indicated that two proteins with molecular weights of 33 and 35 kDa cross-reacting with barley chitinase antibody were induced in rice in response to inoculation with R. solani. The appearance of these chitinases was correlated with increase in enzyme activity.

KW - Compatible interaction

KW - Incompatible interaction

KW - Oryza sativa

KW - PR-proteins

KW - Pestalotia palmarum

KW - Rhizoctonia solani

UR - http://www.scopus.com/inward/record.url?scp=0034901996&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034901996&partnerID=8YFLogxK

U2 - 10.1556/APhyt.36.2001.1-2.9

DO - 10.1556/APhyt.36.2001.1-2.9

M3 - Article

AN - SCOPUS:0034901996

SN - 0238-1249

VL - 36

SP - 67

EP - 74

JO - Acta Phytopathologica et Entomologica Hungarica

JF - Acta Phytopathologica et Entomologica Hungarica

IS - 1-2

ER -

Differential induction of chitinase and Β-1,3-glucanase in response to inoculation with a pathogen (Rhizoctonia solani) and a non-pathogen (Pestalotia palmarum)

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this