Differential induction of chitinase and Β-1,3-glucanase in response to inoculation with a pathogen (Rhizoctonia solani) and a non-pathogen (Pestalotia palmarum)

R. Velazhahan, S. D. Deborah, A. Palaniswami

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Rice leaf sheaths were inoculated with R. solani (pathogen) and P. palmarum (non-pathogen) and were analyzed for the accumulation of pathogenesis-related (PR) proteins. Inoculation of rice plants with R. solani and P. palmarum resulted in a marked increase in activities of chitinase and β-1.3-glucanase. The levels of both enzymes were higher in incompatible interactions than in compatible interactions. Western blot analysis indicated that two proteins with molecular weights of 33 and 35 kDa cross-reacting with barley chitinase antibody were induced in rice in response to inoculation with R. solani. The appearance of these chitinases was correlated with increase in enzyme activity.

Original languageEnglish
Pages (from-to)67-74
Number of pages8
JournalActa Phytopathologica et Entomologica Hungarica
Volume36
Issue number1-2
DOIs
Publication statusPublished - 2001

Fingerprint

Pestalotiopsis palmarum
Thanatephorus cucumeris
chitinase
rice
pathogens
pathogenesis-related proteins
Western blotting
barley
enzyme activity
molecular weight
antibodies
enzymes
leaves
proteins

Keywords

  • Compatible interaction
  • Incompatible interaction
  • Oryza sativa
  • Pestalotia palmarum
  • PR-proteins
  • Rhizoctonia solani

ASJC Scopus subject areas

  • Plant Science
  • Insect Science

Cite this

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title = "Differential induction of chitinase and Β-1,3-glucanase in response to inoculation with a pathogen (Rhizoctonia solani) and a non-pathogen (Pestalotia palmarum)",
abstract = "Rice leaf sheaths were inoculated with R. solani (pathogen) and P. palmarum (non-pathogen) and were analyzed for the accumulation of pathogenesis-related (PR) proteins. Inoculation of rice plants with R. solani and P. palmarum resulted in a marked increase in activities of chitinase and β-1.3-glucanase. The levels of both enzymes were higher in incompatible interactions than in compatible interactions. Western blot analysis indicated that two proteins with molecular weights of 33 and 35 kDa cross-reacting with barley chitinase antibody were induced in rice in response to inoculation with R. solani. The appearance of these chitinases was correlated with increase in enzyme activity.",
keywords = "Compatible interaction, Incompatible interaction, Oryza sativa, Pestalotia palmarum, PR-proteins, Rhizoctonia solani",
author = "R. Velazhahan and Deborah, {S. D.} and A. Palaniswami",
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TY - JOUR

T1 - Differential induction of chitinase and Β-1,3-glucanase in response to inoculation with a pathogen (Rhizoctonia solani) and a non-pathogen (Pestalotia palmarum)

AU - Velazhahan, R.

AU - Deborah, S. D.

AU - Palaniswami, A.

PY - 2001

Y1 - 2001

N2 - Rice leaf sheaths were inoculated with R. solani (pathogen) and P. palmarum (non-pathogen) and were analyzed for the accumulation of pathogenesis-related (PR) proteins. Inoculation of rice plants with R. solani and P. palmarum resulted in a marked increase in activities of chitinase and β-1.3-glucanase. The levels of both enzymes were higher in incompatible interactions than in compatible interactions. Western blot analysis indicated that two proteins with molecular weights of 33 and 35 kDa cross-reacting with barley chitinase antibody were induced in rice in response to inoculation with R. solani. The appearance of these chitinases was correlated with increase in enzyme activity.

AB - Rice leaf sheaths were inoculated with R. solani (pathogen) and P. palmarum (non-pathogen) and were analyzed for the accumulation of pathogenesis-related (PR) proteins. Inoculation of rice plants with R. solani and P. palmarum resulted in a marked increase in activities of chitinase and β-1.3-glucanase. The levels of both enzymes were higher in incompatible interactions than in compatible interactions. Western blot analysis indicated that two proteins with molecular weights of 33 and 35 kDa cross-reacting with barley chitinase antibody were induced in rice in response to inoculation with R. solani. The appearance of these chitinases was correlated with increase in enzyme activity.

KW - Compatible interaction

KW - Incompatible interaction

KW - Oryza sativa

KW - Pestalotia palmarum

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KW - Rhizoctonia solani

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